Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium

Xian-pei Heng; Ke-ji Chen; Zhen-feng Hong; Wei-dong He; Ke-dan Chu; Jiu-mao Lin; Hai-xia Zheng; Liu-qing Yang; Su-ping Huang; Yuan-long Lan; Ling Chen; Fang Guo

doi:10.1007/s11655-011-0945-2

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Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium

Updated：2021-08-27

- Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium
- Chinese Journal of Integrative Medicine Vol. 19, Issue 8, Pages: 596-602(2013)
- Affiliations：
  
  1. Department of Endocrine, People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  3. Fujian Institute of Traditional Chinese Medicine,Fuzhou,China
  4. Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Post-doctoral Science Funds of China (No. 20070410128), CHEN Ke-ji Development Fund of Integrative Medicine (No. CKJ2006001) and Science Fund of Health Department of Fujian Province, China (No. WZY0618)
- DOI：10.1007/s11655-011-0945-2
  CLC：
- Published：2013，
  
  Published Online：9 February 2012，
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Heng, Xp., Chen, Kj., Hong, Zf. et al. Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium., Chin. J. Integr. Med. 19, 596–602 (2013). https://doi.org/10.1007/s11655-011-0945-2

Xian-pei Heng, Ke-ji Chen, Zhen-feng Hong, et al. Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium. [J]. Chinese Journal of Integrative Medicine 19(8):596-602(2013)
Heng, Xp., Chen, Kj., Hong, Zf. et al. Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium., Chin. J. Integr. Med. 19, 596–602 (2013). https://doi.org/10.1007/s11655-011-0945-2 DOI：

Xian-pei Heng, Ke-ji Chen, Zhen-feng Hong, et al. Toxicity features of high glucose on endothelial cell cycle and protection by Dan Gua-Fang (丹瓜方) in ECV-304 in high glucose medium. [J]. Chinese Journal of Integrative Medicine 19(8):596-602(2013) DOI： 10.1007/s11655-011-0945-2.

摘要

To study the toxicity features of high glucose on the endothelial cell cycle and the influence of Dan Gua-Fang (丹瓜方)

a Chinese herbal compound prescription

on the reproductive cycle of vascular endothelial cells cultivated under a high glucose condition; to reveal the partial mechanisms of Dan Gua-Fang in the prevention and treatment of endothelial injury caused by hyperglycemia in diabetes mellitus (DM); and offer a reference for dealing with the vascular complications of DM patients with long-term high blood glucose. Based on the previous 3-(4

5)-dimethylthiahiazo (z-y1)-3–5-diphenytetrazoliumromide (MTT) experiment

under different medium concentrations of glucose and Dangua liquor

the endothelial cells of vein-304 (ECV-304) were divided into 6 groups as follows: standard culture group (Group A

5.56 mmol/L glucose); 1/300 herb-standard group (Group B); high glucose culture group (Group C

16.67 mmol/L glucose); 1/150 herb-high glucose group (Group D); 1/300 herb-high glucose group (Group E); and 1/600 herb-high glucose group (Group F). The cell cycle was assayed using flow cytometry after cells were cultivated for 36

72 and 108 h

respectively. (1) The percentage of cells in the G0/G1 phase was significantly increased in Group C compared with that in Group A (P<0.05)

while the percentage of S-phase (S%) cells in Group C was significantly reduced compared with Group A (P<0.05); the latter difference was dynamically related to the length of growing time of the endothelial cells in a high glucose environment. (2) The S% cells in Group A was decreased by 30.25% (from 40.23% to 28.06%) from 36 h to 72 h

and 12.33% (from 28.06% to 24.60%) from 72 h to 108 h; while in Group C

the corresponding decreases were 23.05% and 21.87%

respectively. The difference of S% cells between the two groups reached statistical significance at 108 h (P<0.05). (3) The percentage difference of cells in the G2/M phase between Group C and Group A was statistically significant at 72 h (P<0.01). (4) 1/300 Dan Gua-Fang completely reversed the harmful effect caused by 16.67 mmol/L high glucose on the cell cycle; moreover it did not disturb the cell cycle when the cell was cultivated in a glucose concentration of 5.56 mmol/L. High glucose produces an independent impact on the cell cycle. Persistent blocking of the cell cycle and its arrest at the G0/G1 phase are toxic effects of high glucose on the endothelial cell cycle. The corresponding variation of the arrest appears in the S phase. 1/300 Dan Gua-Fang completely eliminates the blockage of high glucose on the endothelial cell cycle.

Abstract

To study the toxicity features of high glucose on the endothelial cell cycle and the influence of Dan Gua-Fang (丹瓜方)

a Chinese herbal compound prescription

5)-dimethylthiahiazo (z-y1)-3–5-diphenytetrazoliumromide (MTT) experiment

under different medium concentrations of glucose and Dangua liquor

the endothelial cells of vein-304 (ECV-304) were divided into 6 groups as follows: standard culture group (Group A

5.56 mmol/L glucose); 1/300 herb-standard group (Group B); high glucose culture group (Group C

72 and 108 h

respectively. (1) The percentage of cells in the G0/G1 phase was significantly increased in Group C compared with that in Group A (P<0.05)

and 12.33% (from 28.06% to 24.60%) from 72 h to 108 h; while in Group C

the corresponding decreases were 23.05% and 21.87%

关键词

diabetes mellitusDan Gua-Fangcell cyclevascular lesionconcurrent treatment of phlegm and blood stasisChinese herbal compound

Keywords

diabetes mellitusDan Gua-Fangcell cyclevascular lesionconcurrent treatment of phlegm and blood stasisChinese herbal compound

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