The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562

Ya-li Wang; Ya-li Luo; Che Chen; Neng-lian Li; Ya-li She; Li Zhang

doi:10.1007/s11655-011-0952-3

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The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562

Updated：2021-08-27

- The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562
- Chinese Journal of Integrative Medicine Vol. 18, Issue 5, Pages: 385-390(2012)
- Affiliations：
  
  Gansu College of Traditional Chinese Medicine,Lanzhou,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation for Middle-Aged and Young Scientist of Gansu Province (No. YS031-A21-015)
- DOI：10.1007/s11655-011-0952-3
  CLC：
- Published：2012，
  
  Published Online：12 January 2012，
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Wang, Yl., Luo, Yl., Chen, C. et al. The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562., Chin. J. Integr. Med. 18, 385–390 (2012). https://doi.org/10.1007/s11655-011-0952-3

Ya-li Wang, Ya-li Luo, Che Chen, et al. The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562. [J]. Chinese Journal of Integrative Medicine 18(5):385-390(2012)
Wang, Yl., Luo, Yl., Chen, C. et al. The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562., Chin. J. Integr. Med. 18, 385–390 (2012). https://doi.org/10.1007/s11655-011-0952-3 DOI：

Ya-li Wang, Ya-li Luo, Che Chen, et al. The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562. [J]. Chinese Journal of Integrative Medicine 18(5):385-390(2012) DOI： 10.1007/s11655-011-0952-3.

摘要

To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation

cell cycle

and expressions of p21Ras and proliferating cell nuclear antigen (PCNA) gene in erythroleukemia cell line K562. The effect of total flavonoids of Hedysarum polybotry on K562 cell line survival was determined by the 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium (MTT) reduction assay. The time- and dose-dependent manner was also observed. The cell cycle and apoptosis were analyzed with flow cytometry (FCM). The immunocytochemistry method was applied to quantitatively analyze the effects of flavonoids of Hedysarum polybotry on changes p21Ras and PCNA gene expressions. Flavonoids of Hedysarum polybotry (20–100 μg/mL) significantly inhibited the proliferation of K562 cells in a time- and dose-dependent manner. After K562 cells were cultured for 48 h

total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition (P<0.01)

indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at G0/G1 and G2/M phases. Compared with the control group

p21Ras and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry (40 and 80 μg/mL

respectively) for 48 h. The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry

which might be related to cells arresting.

Abstract

To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation

cell cycle

5-dimethylthiazol-2-yl)-2

total flavonoids of Hedysarum polybotry had no significant effect on the apoptosis of K562 cells but showed significantly inhibition (P<0.01)

indicating that total flavonoids of Hedysarum polybotry could induce K562 cells arrested at G0/G1 and G2/M phases. Compared with the control group

p21Ras and PCNA gene expressions were decreased significantly in K562 cells treated with total flavonoids of Hedysarum polybotry (40 and 80 μg/mL

respectively) for 48 h. The inhibitory effect on proliferation of K562 cells was observed in the groups treated with flavonoids of Hedysarum polybotry

which might be related to cells arresting.

关键词

total flavonoids of Hedysarum polybotryK562 cellscell cyclep21Ras and proliferating cell nuclear antigen

Keywords

total flavonoids of Hedysarum polybotryK562 cellscell cyclep21Ras and proliferating cell nuclear antigen

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