Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats

Ling Yang; Kun Lu; Xiu-ying Wen; Hao Liu; Ai-ping Chen; Ming-wang Xu; Hong Zhang; Jie Yu

doi:10.1007/s11655-011-0959-9

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Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats

Updated：2021-08-27

- Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats
- Chinese Journal of Integrative Medicine Vol. 18, Issue 10, Pages: 775-781(2012)
- Affiliations：
  
  1. Department of Endocrinology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
  2. Department of Traditional Chinese Medicine, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672730), the Research Project of Hubei Provincial Science and Technology Department (No. 2006AA301C24), and the Fundamental Research Funds for the Central Universities, Huazhong University of Science and Technology (No. 2010JC058);These authors contributed equally
- DOI：10.1007/s11655-011-0959-9
  CLC：
- Published：2012，
  
  Published Online：28 March 2012，
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Yang, L., Lu, K., Wen, Xy. et al. Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats., Chin. J. Integr. Med. 18, 775–781 (2012). https://doi.org/10.1007/s11655-011-0959-9

Ling Yang, Kun Lu, Xiu-ying Wen, et al. Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats. [J]. Chinese Journal of Integrative Medicine 18(10):775-781(2012)
Yang, L., Lu, K., Wen, Xy. et al. Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats., Chin. J. Integr. Med. 18, 775–781 (2012). https://doi.org/10.1007/s11655-011-0959-9 DOI：

Ling Yang, Kun Lu, Xiu-ying Wen, et al. Jueming Prescription (决明方) reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats. [J]. Chinese Journal of Integrative Medicine 18(10):775-781(2012) DOI： 10.1007/s11655-011-0959-9.

摘要

To investigate the antiobesity effect of Jueming Prescription (决明方

JMP)

a Chinese herbal medicine formula

and its influence on mRNA expressions of beta3 adrenergic receptor (beta3-AR) and uncoupling protein-2 (UCP-2) in adipose tissue of diet-induced obese rats. Fifty male Sprague-Dawley rats were randomly divided into the normal control group (n =8) that was on a standard chow diet

and the obese model group (n =42) that was on a diet of high fat chow. Two weeks after the high fat diet

29 obese rats in the obese model group were further randomly divided into 3 groups: the untreated obese model group (n =9)

the metformin group (n =10

metformin 300 mg kg−1 day−1)

and the JMP group (n =10

JMP 4 g kg−1 day−1). After 8-week treatment

body weight

wet weight of visceral fat

and percentage of body fat (PBF) were measured. The levels of fasting blood glucose

serum lipids

and insulin were assessed

and insulin sensitivity index (ISI) was calculated. The adipose tissue section was stained with hematoxylin-Eosin

and the cellular diameter and quantity of adipocytes were evaluated by light microscopy. The mRNA expressions of beta3-AR and UCP-2 from the peri-renal fat tissue were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). Compared with the obese model group

treatment with JMP resulted in significantly lower body weight

wet weight of visceral fat

PBF

and diameter of adipocytes

and significantly higher level of high-density lipoprotein cholesterol

ISI (all P<0.01)

JMP increased the mRNA expressions of beta3-AR and UCP-2 from perirenal fat tissue (P <0.05

P<0.01). JMP could reduce body weight and adipocyte size; and the effect was associated with the up-regulation of beta3-AR and UCP-2 expressions in the adipose tissue and improvement of insulin sensitivity.

Abstract

To investigate the antiobesity effect of Jueming Prescription (决明方

JMP)

a Chinese herbal medicine formula

and the obese model group (n =42) that was on a diet of high fat chow. Two weeks after the high fat diet

29 obese rats in the obese model group were further randomly divided into 3 groups: the untreated obese model group (n =9)

the metformin group (n =10

metformin 300 mg kg−1 day−1)

and the JMP group (n =10

JMP 4 g kg−1 day−1). After 8-week treatment

body weight

wet weight of visceral fat

and percentage of body fat (PBF) were measured. The levels of fasting blood glucose

serum lipids

and insulin were assessed

and insulin sensitivity index (ISI) was calculated. The adipose tissue section was stained with hematoxylin-Eosin

treatment with JMP resulted in significantly lower body weight

wet weight of visceral fat

PBF

and diameter of adipocytes

and significantly higher level of high-density lipoprotein cholesterol

ISI (all P<0.01)

JMP increased the mRNA expressions of beta3-AR and UCP-2 from perirenal fat tissue (P <0.05

关键词

Chinese MedicineJueming PrescriptionSemen Cassiaeobesitybeta3 adrenergic receptoruncoupling protein-2

Keywords

Chinese MedicineJueming PrescriptionSemen Cassiaeobesitybeta3 adrenergic receptoruncoupling protein-2

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