Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats

Xia Sun; Qian-qian Lan; Yong Cai; Yan-qin Yu

doi:10.1007/s11655-012-0991-4

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Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats

Updated：2021-08-27

- Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats
- Chinese Journal of Integrative Medicine Vol. 18, Issue 2, Pages: 130-136(2012)
- Affiliations：
  
  1. Department of Basic Medical Science, Institute of Neuroscience, Zhejiang University School of Medicine,Hangzhou,China
  2. Department of Physiology, Hangzhou Key Laboratory of Neurobiology, School of Basic Medicine, Hangzhou Normal University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No.30870834), the Zhejiang Medical and Health Science Research Foundation (No. 2008A042), and the Zhejiang Provincial Natural Science Foundation of China (No. Y2110057 and No. Y2090820)
- DOI：10.1007/s11655-012-0991-4
  CLC：
- Published：2012，
  
  Published Online：5 February 2012，
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Sun, X., Lan, Qq., Cai, Y. et al. Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats., Chin. J. Integr. Med. 18, 130–136 (2012). https://doi.org/10.1007/s11655-012-0991-4

Xia Sun, Qian-qian Lan, Yong Cai, et al. Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats. [J]. Chinese Journal of Integrative Medicine 18(2):130-136(2012)
Sun, X., Lan, Qq., Cai, Y. et al. Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats., Chin. J. Integr. Med. 18, 130–136 (2012). https://doi.org/10.1007/s11655-012-0991-4 DOI：

Xia Sun, Qian-qian Lan, Yong Cai, et al. Electrical stimulation of deep peroneal nerve mimicking acupuncture inhibits the pressor response via capsaicin-insensitive afferents in anesthetized rats. [J]. Chinese Journal of Integrative Medicine 18(2):130-136(2012) DOI： 10.1007/s11655-012-0991-4.

摘要

To assess the inhibitory modulation of blood pressure by stimulation of the deep peroneal nerve (DPN) and to determine the involvement of nociceptive fibers in the modulation. All the animals were divided into six groups (A-F). The rats in groups A and B received no pretreatment. The rats in groups C and D received subcutaneous injection of capsaicin or control vehicle

respectively

near the DPN for 2 days. Those in groups E and F had the DPN exposed to capsaicin or control vehicle

respectively

for 20 min. Subsequently

pressor responses were induced by stimulation of paraventricular nucleus (PVN) either electrically (groups A and C C-F) or chemically via injection of glutamate (group B). After two stable pressor responses (baseline)

all groups were subject to 5-min DPN stimulation followed by PVN stimulation for 10 s. Arterial blood pressure

heart rate

and electrocardiogram were recorded. The pressor response was calculated as the difference in the mean arterial pressure (MAP) before and after PVN stimulation

and changes from baseline in pressor response after DPN stimulation were compared between the groups. Increases of MAP of 22.88±2.18 mm Hg and 20.32±5.25 mm Hg were induced by electrical (group A) or chemical (group B) stimulation of the PVN

respectively. These pressor responses were inhibited by stimulation of the DPN

and the MAP was reduced to 12.00±2.10 mm Hg in group A (n=6

P<0.01) and 7.00±2.85 mm Hg in group B (n=6

P<0.01). Subcutaneous injection of capsaicin (125 mg/kg) near the DPN in group C (n=7) had no effect on the inhibitory effect of DPN stimulation compared with the group D (n=9)

and neither did blockade of nociceptive fibers with capsaicin in group E (n=6) compared with group F (n=8). Stimulation of the DPN mimicking acupuncture has an inhibitory effect on the pressor response

and the effect is mediated by capsaicin-insensitive afferent fibers in the DPN.

Abstract

respectively

near the DPN for 2 days. Those in groups E and F had the DPN exposed to capsaicin or control vehicle

respectively

for 20 min. Subsequently

all groups were subject to 5-min DPN stimulation followed by PVN stimulation for 10 s. Arterial blood pressure

heart rate

and electrocardiogram were recorded. The pressor response was calculated as the difference in the mean arterial pressure (MAP) before and after PVN stimulation

respectively. These pressor responses were inhibited by stimulation of the DPN

and the MAP was reduced to 12.00±2.10 mm Hg in group A (n=6

P<0.01) and 7.00±2.85 mm Hg in group B (n=6

P<0.01). Subcutaneous injection of capsaicin (125 mg/kg) near the DPN in group C (n=7) had no effect on the inhibitory effect of DPN stimulation compared with the group D (n=9)

and the effect is mediated by capsaicin-insensitive afferent fibers in the DPN.

关键词

acupuncturedeep peroneal nerveHypothalamic Paraventricular Nucleuspressor responsecapsaicin

Keywords

acupuncturedeep peroneal nerveHypothalamic Paraventricular Nucleuspressor responsecapsaicin

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Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University

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Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences

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