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Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses
- Chinese Journal of Integrative Medicine Vol. 18, Issue 2, Pages: 120-129(2012)
- Affiliations：
1. The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University,Guangzhou,China
2. Fuda Cancer Hospital,Guangzhou,China
3. Guangdong General Hospital, Guangdong Academy of Medical Sciences,Guangzhou,China
4. The Institute of Integrated Traditional Chinese and Western Medicine, Sun Yatsen University,Guangzhou,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 30572435 and No. 81072776) and Baiyun District Science and Technology Program (No. 2010-KZ-39)
DOI：10.1007/s11655-012-0998-x
CLC：Published：2012，
Published Online：5 February 2012，
Scan for full text
Jia, Yh., Zhang, Yx., Li, Lj. et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses., Chin. J. Integr. Med. 18, 120–129 (2012). https://doi.org/10.1007/s11655-012-0998-x
Yu-hua Jia, Yun-xian Zhang, Li-jun Li, et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses. [J]. Chinese Journal of Integrative Medicine 18(2):120-129(2012)
Jia, Yh., Zhang, Yx., Li, Lj. et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses., Chin. J. Integr. Med. 18, 120–129 (2012). https://doi.org/10.1007/s11655-012-0998-x DOI：
Yu-hua Jia, Yun-xian Zhang, Li-jun Li, et al. Dingxin Recipe (定心方) prevents ischemia/reperfusion-induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses. [J]. Chinese Journal of Integrative Medicine 18(2):120-129(2012) DOI： 10.1007/s11655-012-0998-x.
摘要
To identify the underlying mechanisms of the protective effects of Dingxin Recipe (定: 心方
DXR)
a Chinese compound prescription that has been used clinically in China for more than 20 years
on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. A total of 30 rats were randomly divided into three groups: sham group
I/R group
and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXR-DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days
while rats I/in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrix-matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify assisted differentially expressed proteins. Immunohistochemistry
real-time quantitative polymerase chain reaction (RQ-RQPCR)
Western blot
and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins PCR)
obtained in the above experiments. DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality
as well as arrhythmia score
compared with those of the I/R group. Among successfully identified proteins
prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition
compared with the I/R group
the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment. DXR could alleviate I/R-induced arrhythmias
which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore
up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.
Abstract
To identify the underlying mechanisms of the protective effects of Dingxin Recipe (定: 心方
DXR)
a Chinese compound prescription that has been used clinically in China for more than 20 years
on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. A total of 30 rats were randomly divided into three groups: sham group
I/R group
and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXR-DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days
while rats I/in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrix-matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify assisted differentially expressed proteins. Immunohistochemistry
real-time quantitative polymerase chain reaction (RQ-RQPCR)
Western blot
and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins PCR)
obtained in the above experiments. DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality
as well as arrhythmia score
compared with those of the I/R group. Among successfully identified proteins
prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition
compared with the I/R group
the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment. DXR could alleviate I/R-induced arrhythmias
which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore
up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.
关键词
Dingxin Recipeproteomicsarrhythmiasprohibitinischemia/reperfusioninflammation
Keywords
Dingxin Recipeproteomicsarrhythmiasprohibitinischemia/reperfusioninflammation
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