Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats

Shu-yu Yang; Neng-jiang Zhao; Xue-jun Li; Hui-jie Zhang; Ke-ji Chen; Can-dong Li

doi:10.1007/s11655-012-1023-0

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Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats

Updated：2021-08-27

- Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats
- Chinese Journal of Integrative Medicine Vol. 18, Issue 4, Pages: 262-268(2012)
- Affiliations：
  
  1. Post-Graduate School, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Department of Internal Medicine, the First Affiliated Hospital of Xiamen University,Fujian Province,Xiamen,China
  3. Xiamen Diabetes Institute,Fujian Province,Xiamen,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30973912);and the Innovation Project of Fujian Provincial Health Bureau (No. 2007-CXB-10);and the Project of Technology Bureau of Xiamen City (No. 3502Z20077050)
- DOI：10.1007/s11655-012-1023-0
  CLC：
- Published：2012，
  
  Published Online：30 March 2012，
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Yang, Sy., Zhao, Nj., Li, Xj. et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats., Chin. J. Integr. Med. 18, 262–268 (2012). https://doi.org/10.1007/s11655-012-1023-0

Shu-yu Yang, Neng-jiang Zhao, Xue-jun Li, et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats. [J]. Chinese Journal of Integrative Medicine 18(4):262-268(2012)
Yang, Sy., Zhao, Nj., Li, Xj. et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats., Chin. J. Integr. Med. 18, 262–268 (2012). https://doi.org/10.1007/s11655-012-1023-0 DOI：

Shu-yu Yang, Neng-jiang Zhao, Xue-jun Li, et al. Ping-tang Recipe (平糖方) improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats. [J]. Chinese Journal of Integrative Medicine 18(4):262-268(2012) DOI： 10.1007/s11655-012-1023-0.

摘要

To investigate the therapeutic effects of Ping-tang Recipe (平糖方

PTR) on high-fat diet (HFD)-induced insulin resistance and non-alcoholic fatty liver disease (NAFLD)

and to elucidate the underlying mechanisms. Forty male SD rats were included in the study. Ten rats were fed on normal diet as normal control

and thirty rats were fed on HFD for 8 weeks to induce obesity

followed with low dose (0.42 g/kg) or high dose (0.84 g/kg) of PTR or vehicle for 8 weeks with 10 animals for each group. Glucose metabolism and insulin sensitivity were evaluated by oral glucose tolerance test and insulin tolerance test. Hepatic steatosis was measured by immunohistochemistry. Liver lipid metabolic genes were analyzed by quantitative real-time polymerase chain reaction

while AMP-activated protein kinase (AMPK) expression was examined by Western blot. Rats fed on HFD developed abdominal obesity

insulin resistance and NAFLD. PTR treatment reduced visceral fat (peri-epididymal and peri-renal) accumulation

improved glucose metabolism

and attenuated hepatic steatosis. The expressions of the key lipolytic regulating genes

including peroxisome proliferators-activated receptor γ co-activator 1α (PGC-1α)

peroxisome proliferator-activated receptor γ (PRAR-γ) and α (PRAR-α)

were up-regulated (P<0.05 or P<0.01)

while the expressions of lipogenic genes such as sterol regulatory element-binding protein 1c (SREBP-1c)

fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) were down-regulated (P<0.05 or P<0.01). In addition

PTR activated AMPK and promoted acetyl-CoA carboxylase phosphorylation in the liver. PTR improves insulin resistance and reverse hepatic steatosis in the rat model of HFD-induced obesity through promotion of lipolysis and reduction of lipogenesis

which involves the AMPK signaling pathway

thus representing a new therapeutic intervention for obesity related insulin resistance and NAFLD.

Abstract

To investigate the therapeutic effects of Ping-tang Recipe (平糖方

PTR) on high-fat diet (HFD)-induced insulin resistance and non-alcoholic fatty liver disease (NAFLD)

and to elucidate the underlying mechanisms. Forty male SD rats were included in the study. Ten rats were fed on normal diet as normal control

and thirty rats were fed on HFD for 8 weeks to induce obesity

while AMP-activated protein kinase (AMPK) expression was examined by Western blot. Rats fed on HFD developed abdominal obesity

insulin resistance and NAFLD. PTR treatment reduced visceral fat (peri-epididymal and peri-renal) accumulation

improved glucose metabolism

and attenuated hepatic steatosis. The expressions of the key lipolytic regulating genes

including peroxisome proliferators-activated receptor γ co-activator 1α (PGC-1α)

peroxisome proliferator-activated receptor γ (PRAR-γ) and α (PRAR-α)

were up-regulated (P<0.05 or P<0.01)

while the expressions of lipogenic genes such as sterol regulatory element-binding protein 1c (SREBP-1c)

fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) were down-regulated (P<0.05 or P<0.01). In addition

which involves the AMPK signaling pathway

thus representing a new therapeutic intervention for obesity related insulin resistance and NAFLD.

关键词

Ping-tang Recipenon-alcoholic fatty liver diseaseinsulin resistanceAMP-activated protein kinase

Keywords

Ping-tang Recipenon-alcoholic fatty liver diseaseinsulin resistanceAMP-activated protein kinase

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Pharmaceutical College of Harbin Commercial University

Guangdong Province Integrative Medicine Hospital

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Doctorial Graduate Student of Beijing University of Chinese Medicine

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