Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen

Chuan-ling Tang; Fang Li; Lan Sun; Da-jin Li

doi:10.1007/s11655-012-1025-y

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Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen

Updated：2021-08-27

- Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen
- Chinese Journal of Integrative Medicine Vol. 19, Issue 6, Pages: 439-445(2013)
- Affiliations：
  
  1. Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College,Shanghai,China
  2. Jiangsu Kanion Pharmaceutical Co., Ltd.,Jiangsu Province,Lianyungang,China
  3. Department of Obstetrics and Gynecology, the Affiliated Hospital, Hainan Medical College,Haikou,China
- Author bio：
- Funds：
  
  Supported by National Key Research Program of China (No. 2006CB944007), National and Shanghai Leading Academic Discipline Project (No. 211XK22), Program for Outstanding Medical Academic Leader (to LI Da-jin)
- DOI：10.1007/s11655-012-1025-y
  CLC：
- Published：2013，
  
  Published Online：14 March 2012，
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Tang, Cl., Li, F., Sun, L. et al. Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen., Chin. J. Integr. Med. 19, 439–445 (2013). https://doi.org/10.1007/s11655-012-1025-y

Chuan-ling Tang, Fang Li, Lan Sun, et al. Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen. [J]. Chinese Journal of Integrative Medicine 19(6):439-445(2013)
Tang, Cl., Li, F., Sun, L. et al. Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen., Chin. J. Integr. Med. 19, 439–445 (2013). https://doi.org/10.1007/s11655-012-1025-y DOI：

Chuan-ling Tang, Fang Li, Lan Sun, et al. Therapeutic effect of Bushen Huoxue Recipe (补肾活血方) on autoimmune premature ovarian failure mice established by immunization with recombinant porcine zona pellucida 4 antigen. [J]. Chinese Journal of Integrative Medicine 19(6):439-445(2013) DOI： 10.1007/s11655-012-1025-y.

摘要

To investigate the efficacy and mechanism of Bushen Huoxue Recipe (补肾活血方

BHR) in the treatment of murine autoimmune premature ovarian failure (POF). The recombinant porcine zona pellucida 4 (pZP4) was expressed in E. coli BL21 (DE3) strain within prokaryotic plasmid pET28a (+)

purified by Ni-affinity chromatography and verified by Western blot. Murine autoimmune POF model was established by immunization with pZP4 of female BALB/c mice. Fifty POF mice were randomly divided into 5 groups

which were respectively given low (3.75 mg/kg)

moderate (7.5 mg/kg)

and high dose (15.0 mg/kg) of BHR by gastrogavage once daily for 20 days

with 17-β-estradiol (0.13 mg/kg) and normal saline as positive and negative control. Estrous cycles were analyzed through vaginal smears

serum estradiol (E) levels

and anti-pZP4 antibody titers were detected by ELISA. The proliferative responses in vitro of spleen lymphocytes to pZP4 antigen restimulation were measured by [3H]-thymidine incorporation

and the histomorphology changes of ovary were evaluated by optical microscope. The purified pZP4 was visible as a single lane with 14.4 kD in SDS-PAGE and Western blot. The murine POF model with lengthening estrous cycles

decreased levels of serum E2

high titers of serum anti-pZP4 antibody

and reduced ovarian follicles and corpus lutea were established by immunization with recombinant pZP4. Treatment with moderate and high dosage BHR significantly increased ovarian follicles and reduced the proliferation of spleen lymphocytes to the pZP4 antigen of POF mice (P <0.05). However

only the high dosage BHR administration significantly improved the estrous cycles

elevated the serum E levels (P <0.01)

and decreased the serum anti-pZP4 antibody titers of model mice P<0.05). The recombinant pZP4 could evoke the antigen-specific immune response in mice and induce the autoimmune ovarian injury. It has been demonstrated that BHR was able to increase the serum E levels and protect ovarian functions from the autoimmune injury in murine POF model.

Abstract

To investigate the efficacy and mechanism of Bushen Huoxue Recipe (补肾活血方

which were respectively given low (3.75 mg/kg)

moderate (7.5 mg/kg)

and high dose (15.0 mg/kg) of BHR by gastrogavage once daily for 20 days

with 17-β-estradiol (0.13 mg/kg) and normal saline as positive and negative control. Estrous cycles were analyzed through vaginal smears

serum estradiol (E) levels

and anti-pZP4 antibody titers were detected by ELISA. The proliferative responses in vitro of spleen lymphocytes to pZP4 antigen restimulation were measured by [3H]-thymidine incorporation

decreased levels of serum E2

high titers of serum anti-pZP4 antibody

only the high dosage BHR administration significantly improved the estrous cycles

elevated the serum E levels (P <0.01)

关键词

recombinant porcine zona pellucidaBushen Huoxue RecipePremature Ovarian Failureautoimmunity

Keywords

recombinant porcine zona pellucidaBushen Huoxue RecipePremature Ovarian Failureautoimmunity

references

Goswami D, Conway GS. Premature ovarian failure. Hum Reprod Update 2005;11:391–410.

Beck-Peccoz P, Persani L. Premature ovarian failure. Orphanet J Rare Dis 2006;1:9–13.

Tuohy VK, Altuntas CZ. Autoimmunity and premature ovarian failure. Curr Opin Obstet Gynecol 2007;19:366–369.

Hoek A, Schoemaker J, Drexhage HA. Premature ovarian failure and ovarian autoimmunity. Endocr Rev 1997;18:107–134.

Farquhar C, Marjoribanks J, Lethaby A, Suckling JA, Lamberts Q. Long term hormone therapy for perimenopausal and postmenopausal women. Cochrane Database Syst Rev (Online) 2009;15:CD004143.

Yu N. Traditional Chinese medicine treatment of premature ovarian failure in clinical research. Hunan J Tradit Chin Med (Chin) 2006;24:94–96.

Rhim SH, Millar SE, Robey F, Luo AM, Lou YH, Yule T, et al. Autoimmune disease of the ovary induced by a ZP3 peptide from the mouse zona pellucida. J Clin Invest 1992;89:28–35.

Cai LR, Li DJ, Sun XX, Wang MY. Experimental study on preventive and therapeutic effect of Bushen Huoxue Recipe on autoimmune premature ovarian failure model. Chin J Integr Tradit West Med (Chin) 2001;21:126–129.

Altuntas CZ, Johnson JM, Tuohy VK. Autoimmune targeted disruption of the pituitary-ovarian axis causes premature ovarian failure. J Immunol 2006;177:1988–1996.

Hasegawa A, Koyama K, Okazaki Y, Sugimoto M, Isojima S. Amino acid sequence of a porcine zona pellucida glycoprotein ZP4 determined by peptide mapping and cDNA cloning. J Reprod Fertil 1994;100:245–255.

Hasegawa A, Koyama K, Inoue M, Takemura T, Isojima S. Antifertility effect of active immunization with ZP4 glycoprotein family of porcine zona pellucida in hamsters. J Reprod Immunol 1992;22:197–210.

Zhang L, Qu XF. Progress in traditional Chinese medicine treatment of premature ovarian failure. Inf Tradit Chin Med (Chin) 2009;26:10–12.

Shen W, Yu CQ. Treating premature ovarian failure by reinforcing Kidney. J Chin Integr Med (Chin) 2006;4:653–656.

Zhu L, Luo SP, Xu LM, He YP, Wang L. Effects of Zuogui Pill on immune function in premature ovarian failure mice. China Arch Tradit Chin Med (Chin) 2008;26:1157–1160.

Yang L, Gao N, Xu L, Zhang SJ. Clinical treatment of 57 cases of premature ovarian failure with Yangxue Bushen Decoction. Hebei J Tradit Chin Med (Chin) 2009;31:347–348.

Shen BQ, Su XM. Clinical observation on treatment of 20 cases of premature ovarian failure with Bushen Jianpi Decoction. Sichuan J Tradit Chin Med (Chin) 2009;27:84–85.

Melner MH, Feltus FA. Autoimmune premature ovarian failure—endocrine aspects of a T cell disease. Endocrinology 1999;140:3401–3403.

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Laboratory of Reproductive Immunology, Institute of Obstetrics & Gynecology, Fudan University

Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, Institutes of Biomedical Sciences (IBS), Fudan University Shanghai Medical College

Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases

The Academy of Integrative Medicine of Fudan University

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