Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils

Wei Pang; Xiao-mei Lan; Cheng-bin Wang

doi:10.1007/s11655-012-1054-6

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Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils

Updated：2021-08-27

- Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils
- Chinese Journal of Integrative Medicine Vol. 18, Issue 4, Pages: 283-287(2012)
- Affiliations：
  
  1. Department of Clinical Laboratory Medicine, Chinese People’s Liberation Army General Hospital and Postgraduate Medical School,Beijing,China
  2. Department of Clinical Laboratory Medicine, Affiliate Hospital of Logistical College of Chinese People’s Armed Police Forces,Tianjin,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30873419)
- DOI：10.1007/s11655-012-1054-6
  CLC：
- Published：2012，
  
  Published Online：30 March 2012，
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Pang, W., Lan, Xm. & Wang, Cb. Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils., Chin. J. Integr. Med. 18, 283–287 (2012). https://doi.org/10.1007/s11655-012-1054-6

Wei Pang, Xiao-mei Lan, Cheng-bin Wang. Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils. [J]. Chinese Journal of Integrative Medicine 18(4):283-287(2012)
Pang, W., Lan, Xm. & Wang, Cb. Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils., Chin. J. Integr. Med. 18, 283–287 (2012). https://doi.org/10.1007/s11655-012-1054-6 DOI：

Wei Pang, Xiao-mei Lan, Cheng-bin Wang. Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils. [J]. Chinese Journal of Integrative Medicine 18(4):283-287(2012) DOI： 10.1007/s11655-012-1054-6.

摘要

To investigate the effect of puerarin on interleukin (IL)-8 mRNA expression and the protein release in the co-culture of human bronchial epithelial (BEAS-2B) cells and human neutrophils. BEAS-2B cells and neutrophills were cultured separately and co-cultured with puerarin (50

100

and 200 μg/mL) for a predetermined time. Cytokines in culture supernatant were evaluated by protein array and IL-8 quantified by enzyme-linked immunosorbent assay (ELISA). IL-8 mRNA expression was evaluated by real-time quantitative polymerase chain reaction (real-time qPCR). The co-culture of BEAS-2B cells and neutrophils exhibited synergistic effects on IL-8 mRNA expression in BEAS-2B cells

but not in neutrophils after 12 h incubation (P<0.01)

as compared with that in BEAS-2B cells or neutrophils alone. IL-8 protein release in the culture supernatant was obviously elevated when BEAS-2B cells were co-cultured with human neutrophils as compared with that in the supernatant of BEAS-2B cells or neutrophils alone after incubated for 2

and 18 h (P<0.01). Treatment with puerarin could significantly down-regulate the expression of IL-8 mRNA in BEAS-2B cells and IL-8 release in the supernatant of the co-culture of BEAS-2B cells and neutrophils (P<0.01). Puerarin could exhibit anti-inflammatory activity by suppressing IL-8 production from the co-culture of human bronchial epithelial cells and neutrophils.

Abstract

100

but not in neutrophils after 12 h incubation (P<0.01)

关键词

bronchial epithelial cellsinterleukin-8neutrophilsPuerarin

Keywords

bronchial epithelial cellsinterleukin-8neutrophilsPuerarin

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Deparment of Laboratory Medicine, Wenzhou Medical College

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