Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells

Guo-yin Zheng; Hai-liang Xin; Bai Li; Yan-feng Xu; Ting-jiao Yi; Chang-quan Ling

doi:10.1007/s11655-012-1055-5

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Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells

Updated：2021-08-27

- Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells
- Chinese Journal of Integrative Medicine Vol. 18, Issue 3, Pages: 197-202(2012)
- Affiliations：
  
  Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. C0305020), the Traditional Chinese Medicine Modernization Project of Science and Technology Commission of Shanghai Municipality (No. 04DZ19808), and the Open Project Foundation of Key Laboratory of Liver and Kidney Diseases (Shanghai University of Traditional Chinese Medicine), Ministry of Education
- DOI：10.1007/s11655-012-1055-5
  CLC：
- Published：2012，
  
  Published Online：2 April 2012，
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Zheng, Gy., Xin, Hl., Li, B. et al. Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells., Chin. J. Integr. Med. 18, 197–202 (2012). https://doi.org/10.1007/s11655-012-1055-5

Guo-yin Zheng, Hai-liang Xin, Bai Li, et al. Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells. [J]. Chinese Journal of Integrative Medicine 18(3):197-202(2012)
Zheng, Gy., Xin, Hl., Li, B. et al. Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells., Chin. J. Integr. Med. 18, 197–202 (2012). https://doi.org/10.1007/s11655-012-1055-5 DOI：

Guo-yin Zheng, Hai-liang Xin, Bai Li, et al. Total saponin from root of Actinidia Valvata Dunn prevents the metastasis of human hepatocellular carcinoma cells. [J]. Chinese Journal of Integrative Medicine 18(3):197-202(2012) DOI： 10.1007/s11655-012-1055-5.

摘要

To extract the active component from the root of Actinidia valvata Dunn and to investigate the effects on hepatocellular carcinoma (HCC) cells in vitro. Total saponin was extracted from the root of A. valvata (TSAVD). HCC cells

such as BEL-7402

HepG2

PLC

SMMC-7721

MHCC-97-H

and MHCC-97-L

were treated with TSAVD in 3-(4

5-Dimethylthiazol-2-yl)-2

5-diphenytetrazolium bromide (MTT) assay. BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays

and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed. Meanwhile

the effects of TSAVD on invasion and migration of BEL-7402 and MHCC-97-H cells were also investigated by transwell chamber in invasion and migration assays. TSAVD at 1.5 mg/mL inhibited BEL-7402 cell proliferation with inhibition ratios (IRs) of 61.08%

74.12%

84.55% at 24

and 72 h

respectively. Meanwhile

TSAVD inhibited MHCC-97-H proliferation in a concentration-dependent manner from 1.5 to 0.5 mg/mL

with the IR of 36% at 1.5 mg/mL at 24 h. For SMMC-7721

PLC

and HepG2

the IR was lower than 30% at 1.5 mg/mL at 24 h. In the wound healing assay

mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group. After pretreatment for 24 h with TSAVD

adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells

with IRs of 48.50%±4.86% and 49.85%±5.25% at 200 μg/mL. The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91% and 79.37%±0.09% at 200 μg/mL. In the invasion assay

IRs were 69.78%±4.88% and 82.48%±0.25% at 200 μg/mL. Of all HCC cells

the highest inhibition by TSAVD was seen for BEL-7402 proliferation. TSAVD could restrain adhesion

invasion

mobility

and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.

Abstract

such as BEL-7402

HepG2

PLC

SMMC-7721

MHCC-97-H

and MHCC-97-L

were treated with TSAVD in 3-(4

5-Dimethylthiazol-2-yl)-2

5-diphenytetrazolium bromide (MTT) assay. BEL-7402 and MHCC-97-H cells were also treated respectively with TSAVD at different concentrations for 24 h in wound healing and adhesion assays

and the effects of TSAVD on BEL-7402 and MHCC-97-H cells mobility and adhesion abilities were observed. Meanwhile

74.12%

84.55% at 24

and 72 h

respectively. Meanwhile

TSAVD inhibited MHCC-97-H proliferation in a concentration-dependent manner from 1.5 to 0.5 mg/mL

with the IR of 36% at 1.5 mg/mL at 24 h. For SMMC-7721

PLC

and HepG2

the IR was lower than 30% at 1.5 mg/mL at 24 h. In the wound healing assay

mobility abilities of BEL-7402 and MHCC-97-H cells in TSAVD treated groups were significantly weaker than those of the control group. After pretreatment for 24 h with TSAVD

adhesion abilities were reduced in both MHCC-97-H and BEL-7402 cells

with IRs of 48.50%±4.86% and 49.85%±5.25% at 200 μg/mL. The IRs of MHCC-97-H and BEL-7402 cells in the migration assay were 49.13%±2.91% and 79.37%±0.09% at 200 μg/mL. In the invasion assay

IRs were 69.78%±4.88% and 82.48%±0.25% at 200 μg/mL. Of all HCC cells

the highest inhibition by TSAVD was seen for BEL-7402 proliferation. TSAVD could restrain adhesion

invasion

mobility

and migration abilities of BEL-7402 and MHCC-97-H cells in vitro.

关键词

Actinidia valvata Dunnhepatocellular carcinomametastasismobilityadhesioninvasionmigration

Keywords

Actinidia valvata Dunnhepatocellular carcinomametastasismobilityadhesioninvasionmigration

references

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Ling CQ. Problems in cancer treatment and major research of integrative medicine. J Chin Integr Med (Chin) 2003;1:168–170.

Zhang YN, Lu L, Ling CQ. Inhibition effect of active fraction from Actinidia valvata on growth of transplanted mouse tumor cells and preliminary study of its mechanism. China J Chin Mater Med (Chin) 2006;31:918–920.

Lu Y, Chen SY, Wu P, Chan G, Fu CX. Studies on the effective components of Actinidia macrosperma (Actinidiaceae) (I)-Analysis on the inorganic elements. J Zhejiang Univ (Agric Life Sci, Chin) 2004;30:185–188.

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Liu S, Yu M, He Y, Xiao L, Wang F, Song C, et al. Melittin prevents liver cancer cell metastasis through inhibition of Rac1-dependent pathway. Hepatology 2008;47:1964–1973.

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