In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation

Ming-yu Zhang; Hui Qiang; Hua-qing Yang; Xiao-qian Dang; Kun-zheng Wang

doi:10.1007/s11655-012-1056-4

Your Location：

Home >

Browse articles >

In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation

Updated：2021-08-27

- In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation
- Chinese Journal of Integrative Medicine Vol. 18, Issue 4, Pages: 276-282(2012)
- Affiliations：
  
  1. Department of Sport Injury, the Affiliated Red Cross Hospital of Medical College of Xi’an Jiaotong University,Xi’an,China
  2. Department of Orthopedics, Shaanxi Provincial People’s Hospital,Xi’an,China
  3. Department of Orthopedics, the Second Hospital of Tsinghua University,Beijing,China
  4. Department of Orthopedics, the Second Affiliated Hospital of Medical College of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the Doctoral Fund of Ministry of Education of China (No. 20070698083)
- DOI：10.1007/s11655-012-1056-4
  CLC：
- Published：2012，
  
  Published Online：30 March 2012，
Scan for full text
Zhang, My., Qiang, H., Yang, Hq. et al. , In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation., Chin. J. Integr. Med. 18, 276–282 (2012). https://doi.org/10.1007/s11655-012-1056-4

Ming-yu Zhang, Hui Qiang, Hua-qing Yang, et al. In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation. [J]. Chinese Journal of Integrative Medicine 18(4):276-282(2012)
Zhang, My., Qiang, H., Yang, Hq. et al. , In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation., Chin. J. Integr. Med. 18, 276–282 (2012). https://doi.org/10.1007/s11655-012-1056-4 DOI：

Ming-yu Zhang, Hui Qiang, Hua-qing Yang, et al. In vitro and in vivo effects of puerarin on promotion of osteoblast bone formation. [J]. Chinese Journal of Integrative Medicine 18(4):276-282(2012) DOI： 10.1007/s11655-012-1056-4.

摘要

To assess the effect of puerarin

a natural flavonoid found in Chinese Pueraria Lobata (Wild.) Ohwi

on promotion of new bone formation. Osteoblasts isolated from calvarial of newborn rats were cultured in vitro in the presence of puerarin at various concentrations. The viability of osteoblasts and alkaline phosphotase activity and mineral node formation were determined. In addition

osteoblasts seeded in the β-tricaclium phosphate scalfolds as bone substitute were implanted in rat dorsal muscles. Half -of the recipient rats received intramuscular injection of puerarin at 10 mg/(kg·d) for 7 days. Osteogenesis was analyzed by examining the histology after 4 weeks of implantation. The viability of osteoblasts treated with puerarin at either 40 or 80 μmol/L was significantly higher than that of the control (P<0.05 and P<0.01

respectively). Alkaline phosphatase and mineral modules were significantly increased in osteoblasts cultured with puerarin at 40 or 80 mol/L when compared with that of the untreated cells. The puerarin-treated rats had a higher rate of bone formation in the osteoblast implants than the control rats (6.35% vs. 1.32%

respectively

P<0.05). Puerarin was able to affect osteoblast proliferation and differentiation

and promote the new bone formation in osteoblast implants.

Abstract

To assess the effect of puerarin

a natural flavonoid found in Chinese Pueraria Lobata (Wild.) Ohwi

respectively

P<0.05). Puerarin was able to affect osteoblast proliferation and differentiation

and promote the new bone formation in osteoblast implants.

关键词

Puerarinosteoblastβ-tricalcium phosphatenew bone formation

Keywords

Puerarinosteoblastβ-tricalcium phosphatenew bone formation

references

Hirokazu K, Takaaki T, Masaaki C, Takahiro K. Repair of segmental bone defects in rabbit tibiae using a complex of b-tricalcium phosphate, type I collagen, and fibroblast growth factor-2. Biomaterials 2006;27:5118–5126.

Kneser U, Schaefer DJ, Polykandriotis E, Horch RE. Tissue engineering of bone: the reconstructive surgeon’s point of view. J Cell Mol Med 2006;10:7–19.

Smith LA, Liu XH, Ma PX. Nano-fibrous scaffolds for tissue engineering. Soft Matter 2008;4:2144–2149.

Liang G, Yang Y, Oh S, Ong JL, Zheng C, Ran J, et al. Ectopic osteoinduction and early degradation of recombinant human bone morphogenetic protein-2-loaded porous beta-tricalcium phosphate in mice. Biomaterials 2005;26:4265–4271.

Langer R, Vacanti JP. Tissue engineering. Science 1993;260:920–926.

Eppley BL, Pietrzak WS, Blanton MW. Allograft and alloplastic bone substitutes: a review of science and technology for the craniomaxillofacial surgeon. J Craniofac Surg 2005;16:981–989.

Kneser U, Schaefer DJ, Polykandriotis E, Horch RE. Tissue engineering of bone: the reconstructive surgeon’s point of view. J Cell Mol Med 2006;10:7–19.

Anderson JB, Garner SC. Phytoestrogens and bone. Baillieres Clin Endocrinol Metab 1998;12:543–557.

Wu HQ, Guo HN, Wang HQ, Chang MZ, Zhang GL, Zhao YX. Protective effect and mechanism of puerarin on learning-memory disorder after global cerebral ischemiareperfusion injury in rats. Chin J Integr Med 2009;15:54–59.

Wang XX, Wu J, Chiba H, Umegaket K, Yamada K, Ishimi Y. Puerariae radix prevents bone loss in ovariectomized mice. J Bone Miner Metab 2003;21:268–275.

Blarir, HC, Jordan SE, Peterson TG, Stephenal B. Variable effects of ty-rosine kinase inhibitor on avian osteoclastic activity and reduction of bone loss in ovariectiomized rats. J Cell Biochem 1996;61:629–637.

Heidi D, Natasj VV, Erna D, Maeyer Ronald V, Etienne S, Leo DR, et al. Isolation, proliferation and differentiation of osteoblastic cells to study cell/biomaterial interactions: comparison of different isolation techniques and source. Biomaterials 2004;25:757–768.

Yuan J, Cui L, Zhang WJ, Liu W, Cao Y. Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate. Biomaterials 2007;28:1005–1013.

Zhou Q, Fu T. Effect of puerarin on the healing osteoporotic fracture in ovariectomized rats. Chin J Clin Rehabilit 2006;10:45–47.

Habibovic P, Yuan H, van der Valk CM, Meijer G, van Blitterswijk CA, de Groot K. 3D microenvironment as essential element for osteoinduction by biomaterials. Biomaterials 2005;26:3565–3575.

Dang ZC, Audinot V, Papapoulos SE, Boutin JA, Löwik CWGM. Peroxisome proliferator-activated receptor g (PPARg) as a molecular target for the soy phytoestrogen genistein. J Biol Chem 2003;278:962–967.

Dang ZC, Löwik CW. The balance between concurrent activation of ERs and PPARs determines daidzeininduced osteogenesis and adipogenesis. J Bone Miner Res 2004;19:853–861.

Viereck V, Gründker C, Blaschke S, Siggelkow H, Emons G, Hofbauer LC. Phytoestrogen genistein stimulates the production of osteoprotegerin by human trabecular osteoblasts. J Cell Biochem 2002;84:725–735.

Notoya M, Tsukamoto Y, Nishimura H, Woo JT, Nagai K, Lee IS, et al. Quercetin, a flavonoid, inhibits the proliferation, differentiation, and mineralization of osteoblasts in vitro. Eur J Pharmacol 2004;485:89–96.

Garcia T, Roman S, Jackson A, Thellhaber J, Connolly T, Spinella-Jaegle S, et al. Behavior of osteoblast, adipocyte, and myoblast markers in genome-wide expression analysis of mouse calvaria primary osteoblasts in vitro. Bone 2002;31:205–211.

Whyte MP. Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocr Rev 1994;15:439–461.

Fedarko NS, Bianco P, Vetter U, Gehron Robey P. Human bone cell enzyme expression and cellular heterogeneity: correlation of alkaline phosphatase enzyme activity with cell cycle. J Cell Physiol 1990;144:115–121.

Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA. Mineralization and expression of matrix proteins during in vivo bone development. Calcif Tissue Int 1998;62:74–82.

Maenoa S, Nikia Y, Matsumotoa H, Morioka H, Yatabe T, Funayama A, et al. The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials 2005;26:4847–4855.

Xu SL, Li DC, Xie YZ, Lu JX, Dai KR. The growth of stem cells within β-TCP scaffolds in a fluid-dynamic environment. Mater Sci Engin 2008;C28:164–170.

FULL TEXT LINK

More>

Views

767

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways

Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells

Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils

Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path

Protective effects and mechanism of puerarin on learning-memory disorder after global cerebral ischemia-reperfusion injury in rats

Related Author

No data

Related Institution

Deparment of Laboratory Medicine, Wenzhou Medical College

Department of Clinical Laboratory, Chinese People’s Liberation Army General Hospital

Institute of Hematology, the First Hospital Affiliated to Zhejiang University of Chinese Medicine

Department of Clinical Laboratory Medicine, Affiliate Hospital of Logistical College of Chinese People’s Armed Police Forces

Department of Clinical Laboratory Medicine, Chinese People’s Liberation Army General Hospital and Postgraduate Medical School

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰