Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path

Ying Zhao; You Zhou

doi:10.1007/s11655-012-1058-2

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Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path

Updated：2021-08-27

- Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path
- Chinese Journal of Integrative Medicine Vol. 18, Issue 4, Pages: 293-298(2012)
- Affiliations：
  
  Pharmaceutical College of Harbin Commercial University,Harbin,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-012-1058-2
  CLC：
- Published：2012，
  
  Published Online：30 March 2012，
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Zhao, Y., Zhou, Y. Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path., Chin. J. Integr. Med. 18, 293–298 (2012). https://doi.org/10.1007/s11655-012-1058-2

Ying Zhao, You Zhou. Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path. [J]. Chinese Journal of Integrative Medicine 18(4):293-298(2012)
Zhao, Y., Zhou, Y. Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path., Chin. J. Integr. Med. 18, 293–298 (2012). https://doi.org/10.1007/s11655-012-1058-2 DOI：

Ying Zhao, You Zhou. Puerarin improve insulin resistance of adipocyte through activating Cb1 binding protein path. [J]. Chinese Journal of Integrative Medicine 18(4):293-298(2012) DOI： 10.1007/s11655-012-1058-2.

摘要

To explore the molecular mechanism of puerarin (Pue) in improving insulin resistance through observing its effect on the insulin resistance of 3T3-Li lipocyte induced by free fatty acid (FFA). 3T3-L1 preadipocyte was induced by a culture solution containing insulin

isobutyo-menthyl-xanthine

and dexamethasone to mature lipocyte

and it was divided into six groups: the control group (normal cells)

the model group (untreated model cells)

and the four drug treatment group exposed to dimethyl biguanide (Met group)

highdose puerarin (PueH group)

low-dose puerarin (PueL group)

and propylene glycol (PG group)

respectively. Mature lipocytes in various groups

except those in the normal group

were established into insulin resistance model by FFA induction and treated respectively with corresponding drugs. Peroxisome proliferator-activated receptor-γ (PPAR-γ) mRNA expressions at the fourth

sixth

and eighth day were observed using reverse transcription polymerase chain reaction (RT-PCR); glucose transportation in various groups were observed by 2-deoxy-[3H]-D-glucose intake method; mRNA expression of Cb1 binding protein (CAP) was determined by RT-PCR; and glucose transporter-4 (Glut-4) transposition was detected by immune-fluorescence method. PPAR-γmRNA expression increased gradually

and it showed lower levels at the fourth

sixth

and eighth day in all treatment groups than that in the model group. Glucose transportation determination showed that the transportation in the model group was 2.23±0.63

significantly lower than that in the normal group 5.05±0.66 (P<0.01); as compared with the model group

they were significantly higher in the PueH and the PueL groups. In addition

the CAP mRNA expression and membranous distribution of Glut-4 were higher in the two Pue treated groups than those in the model group

respectively. Pue could markedly improve the insulin resistance of 3T3-L1 lipocyte

which is realized possibly by way of inactivating CAP path

promoting Glut-4 transposition to cell membrane to increase the transportation of glucose.

Abstract

isobutyo-menthyl-xanthine

and dexamethasone to mature lipocyte

and it was divided into six groups: the control group (normal cells)

the model group (untreated model cells)

and the four drug treatment group exposed to dimethyl biguanide (Met group)

highdose puerarin (PueH group)

low-dose puerarin (PueL group)

and propylene glycol (PG group)

respectively. Mature lipocytes in various groups

except those in the normal group

sixth

and it showed lower levels at the fourth

sixth

and eighth day in all treatment groups than that in the model group. Glucose transportation determination showed that the transportation in the model group was 2.23±0.63

significantly lower than that in the normal group 5.05±0.66 (P<0.01); as compared with the model group

they were significantly higher in the PueH and the PueL groups. In addition

the CAP mRNA expression and membranous distribution of Glut-4 were higher in the two Pue treated groups than those in the model group

respectively. Pue could markedly improve the insulin resistance of 3T3-L1 lipocyte

which is realized possibly by way of inactivating CAP path

promoting Glut-4 transposition to cell membrane to increase the transportation of glucose.

关键词

Puerarininsulin resistanceperoxisome vegetation activating receptor-γCb1 binding proteinglucose transporter-4

Keywords

Puerarininsulin resistanceperoxisome vegetation activating receptor-γCb1 binding proteinglucose transporter-4

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Deparment of Laboratory Medicine, Wenzhou Medical College

Department of Clinical Laboratory, Chinese People’s Liberation Army General Hospital

Xiamen Diabetes Institute

Department of Internal Medicine, the First Affiliated Hospital of Xiamen University

Post-Graduate School, Fujian University of Traditional Chinese Medicine

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