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Tumor interstitial fluid and gastric cancer metastasis: An experimental study to verify the hypothesis of “tumor-phlegm microenvironment”
- Chinese Journal of Integrative Medicine Vol. 18, Issue 5, Pages: 350-358(2012)
- Affiliations：
1. Institute of Traditional Chinese Medicine Research, Chinese People’s Liberation Army General Hospital,Beijing,China
2. Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University,Shanghai,China
3. Department of Pathology, Changzheng Hospital, Second Military Medical University,Shanghai,China
- Author bio：
- Funds：Supported by the Major Research Program Grant of the National Science Foundation (No. 90709044), and China Postdoctoral Science Foundation Project (No. 20100480096)
DOI：10.1007/s11655-012-1085-z
CLC：Published：2012，
Published Online：2 May 2012，
Scan for full text
Sun, Dz., Jiao, Jp., Ju, Dw. et al. Tumor interstitial fluid and gastric cancer metastasis: An experimental study to verify the hypothesis of “tumor-phlegm microenvironment”., Chin. J. Integr. Med. 18, 350–358 (2012). https://doi.org/10.1007/s11655-012-1085-z
Da-zhi Sun, Jian-peng Jiao, Da-wei Ju, et al. Tumor interstitial fluid and gastric cancer metastasis: An experimental study to verify the hypothesis of “tumor-phlegm microenvironment”. [J]. Chinese Journal of Integrative Medicine 18(5):350-358(2012)
Sun, Dz., Jiao, Jp., Ju, Dw. et al. Tumor interstitial fluid and gastric cancer metastasis: An experimental study to verify the hypothesis of “tumor-phlegm microenvironment”., Chin. J. Integr. Med. 18, 350–358 (2012). https://doi.org/10.1007/s11655-012-1085-z DOI：
Da-zhi Sun, Jian-peng Jiao, Da-wei Ju, et al. Tumor interstitial fluid and gastric cancer metastasis: An experimental study to verify the hypothesis of “tumor-phlegm microenvironment”. [J]. Chinese Journal of Integrative Medicine 18(5):350-358(2012) DOI： 10.1007/s11655-012-1085-z.
摘要
To extract tumor interstitial fluid (TIF) from MKN-45 gastric cancer which is similar to “muddy phlegm” in Chinese medicine and observe influences of MKN-45 tumor interstitial fluid (MKN-45 TIF) intervention on metastasis of gastric cancer and on the expressions of vascular endothelial growth factor (VEGF)
kinase insert domain containing receptor (KDR)
epithelial-cadherin (E-cad)
cyclooxygenase-2 (COX-2)
intercellular adhesion molecule-1 (ICAM-1) and telomerase genes and proteins in primary tumor tissue. An MKN-45 tumor-bearing model was established in 50 nude mice. The modeled animals were equally randomized to 5 groups: the simple tumor-bearing group (model group)
the normal saline (NS) via tail vein injection (i.v.) group (NS i.v. group)
MKN-45 TIF i.v. group (TIF i.v. group)
NS intraperitoneal injection (i.p.) group (NS i.p. group)
and MKN-45 TIF i.p. group (TIF i.p. group). The TIF and NS intervention groups received injection (i.p. or i.v.) of MKN-45 TIF or NS twice a week
0.2 mL at a time. After 8 weeks
the primary tumors were removed
weighed and HE stained to observe tumor metastasis. The primary tumor tissues were analyzed by immunohistochemistry and real-time quantitative PCR to detect expressions of VEGF
KDR
E-cad
COX-2
ICAM-1
and telomerase genes and proteins in different groups. There were significant differences in tumor weight between TIF intervention groups and the model and NS intervention groups. Tumor metastasis was observed in all 5 groups
but the tumor metastasis rate in TIF intervention groups was significantly higher than those in the model and NS intervention groups. The gene and protein expressions of gastric cancer-related factors VEGF
KDR
COX-2
ICAM-1 and telomerase were unregulated while the gene and protein expressions of E-cad were downregulated in TIF intervention groups. TIF promotes tumor growth
invasion and metastasis of gastric cancer. These findings provide preliminary experimental clues for verifying the hypothesis of “tumor-phlegm microenvironment”.
Abstract
To extract tumor interstitial fluid (TIF) from MKN-45 gastric cancer which is similar to “muddy phlegm” in Chinese medicine and observe influences of MKN-45 tumor interstitial fluid (MKN-45 TIF) intervention on metastasis of gastric cancer and on the expressions of vascular endothelial growth factor (VEGF)
kinase insert domain containing receptor (KDR)
epithelial-cadherin (E-cad)
cyclooxygenase-2 (COX-2)
intercellular adhesion molecule-1 (ICAM-1) and telomerase genes and proteins in primary tumor tissue. An MKN-45 tumor-bearing model was established in 50 nude mice. The modeled animals were equally randomized to 5 groups: the simple tumor-bearing group (model group)
the normal saline (NS) via tail vein injection (i.v.) group (NS i.v. group)
MKN-45 TIF i.v. group (TIF i.v. group)
NS intraperitoneal injection (i.p.) group (NS i.p. group)
and MKN-45 TIF i.p. group (TIF i.p. group). The TIF and NS intervention groups received injection (i.p. or i.v.) of MKN-45 TIF or NS twice a week
0.2 mL at a time. After 8 weeks
the primary tumors were removed
weighed and HE stained to observe tumor metastasis. The primary tumor tissues were analyzed by immunohistochemistry and real-time quantitative PCR to detect expressions of VEGF
KDR
E-cad
COX-2
ICAM-1
and telomerase genes and proteins in different groups. There were significant differences in tumor weight between TIF intervention groups and the model and NS intervention groups. Tumor metastasis was observed in all 5 groups
but the tumor metastasis rate in TIF intervention groups was significantly higher than those in the model and NS intervention groups. The gene and protein expressions of gastric cancer-related factors VEGF
KDR
COX-2
ICAM-1 and telomerase were unregulated while the gene and protein expressions of E-cad were downregulated in TIF intervention groups. TIF promotes tumor growth
invasion and metastasis of gastric cancer. These findings provide preliminary experimental clues for verifying the hypothesis of “tumor-phlegm microenvironment”.
关键词
tumor interstitial fluidGastric Cancertumor metastasistumor metastasis-related genes and proteins
Keywords
tumor interstitial fluidGastric Cancertumor metastasistumor metastasis-related genes and proteins
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