Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head

Wei-heng Chen; Xiang-ying Kong; Rong Wan; Chun-sheng Xiao; Li Li; Zhi-yao Wang; Na Lin; He-ming Wang

doi:10.1007/s11655-012-1086-y

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Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head

Updated：2021-08-27

- Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head
- Chinese Journal of Integrative Medicine Vol. 18, Issue 5, Pages: 378-384(2012)
- Affiliations：
  
  1. Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing,China
  3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Foundation of China (No. 81173417 and 30472131)
- DOI：10.1007/s11655-012-1086-y
  CLC：
- Published：2012，
  
  Published Online：2 May 2012，
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Chen, Wh., Kong, Xy., Wan, R. et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head., Chin. J. Integr. Med. 18, 378–384 (2012). https://doi.org/10.1007/s11655-012-1086-y

Wei-heng Chen, Xiang-ying Kong, Rong Wan, et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head. [J]. Chinese Journal of Integrative Medicine 18(5):378-384(2012)
Chen, Wh., Kong, Xy., Wan, R. et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head., Chin. J. Integr. Med. 18, 378–384 (2012). https://doi.org/10.1007/s11655-012-1086-y DOI：

Wei-heng Chen, Xiang-ying Kong, Rong Wan, et al. Effects of Huogu I Formula (活骨I方) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head. [J]. Chinese Journal of Integrative Medicine 18(5):378-384(2012) DOI： 10.1007/s11655-012-1086-y.

摘要

To study the mechanism of Huogu I Formula (活骨I方) in treating osteonecrosis of femoral head. Forty-eight healthy female Leghorn chickens were randomly divided into control group

model group and Huogu I group

and each group consisted of 16 chickens. At the meantime of model establishment

chickens of the Huogu I group were administrated with decoction

while the model and control group with distilled water by gavage. At the 8th and 16th week after medication

blood samples were obtained for blood lipid detection while both sides of femoral head were harvested for the rest of examinations. Specifically

expressions of bone morphogenetic protein-2 (BMP2)

transforming growth factor beta1 (TGFβ1)

Smad4 and Smad7 were evaluated by immunohistochemistry

while expression of osteoprotegerin/receptor activator of nuclear factor kappaB ligand (OPG/RANKL) mRNA was detected by in situ hybridization. Compared with the control group

serum levels of total cholesterol (TC)

triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the model group rose significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the model group dropped prominently. Positive cell counting of Smad7 and RANKL increased dramatically. In contrast with the model group

levels of TC

TG and LDL-C in Huogu I group reduced significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the Huogu I group increased prominently. Indices of Smad7 and RANKL both decreased significantly. Especially at the 8th week

these variations were more significant. Huogu I Formula is effective in promoting repair of necrotic femoral head by regulating the expressions of BMP2

TGFβ1

Smads and OPG/RANKL of osteoclast in femoral head.

Abstract

To study the mechanism of Huogu I Formula (活骨I方) in treating osteonecrosis of femoral head. Forty-eight healthy female Leghorn chickens were randomly divided into control group

model group and Huogu I group

and each group consisted of 16 chickens. At the meantime of model establishment

chickens of the Huogu I group were administrated with decoction

while the model and control group with distilled water by gavage. At the 8th and 16th week after medication

blood samples were obtained for blood lipid detection while both sides of femoral head were harvested for the rest of examinations. Specifically

expressions of bone morphogenetic protein-2 (BMP2)

transforming growth factor beta1 (TGFβ1)

Smad4 and Smad7 were evaluated by immunohistochemistry

while expression of osteoprotegerin/receptor activator of nuclear factor kappaB ligand (OPG/RANKL) mRNA was detected by in situ hybridization. Compared with the control group

serum levels of total cholesterol (TC)

triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the model group rose significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the model group dropped prominently. Positive cell counting of Smad7 and RANKL increased dramatically. In contrast with the model group

levels of TC

TG and LDL-C in Huogu I group reduced significantly. Positive cell counting of BMP2

TGFβ1

Smad4 and OPG in femoral head of the Huogu I group increased prominently. Indices of Smad7 and RANKL both decreased significantly. Especially at the 8th week

these variations were more significant. Huogu I Formula is effective in promoting repair of necrotic femoral head by regulating the expressions of BMP2

TGFβ1

Smads and OPG/RANKL of osteoclast in femoral head.

关键词

osteonecrosis of femoral headbone morphogenetic protein-2transforming growth factor beta1osteoprotegerinreceptor activator of nuclear factor kappaBSmads

Keywords

osteonecrosis of femoral headbone morphogenetic protein-2transforming growth factor beta1osteoprotegerinreceptor activator of nuclear factor kappaBSmads

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College of Pharmaceutical Science and Technology, Tianjin University

Tianjin Chase Sun Pharmaceutical Co., Ltd.

Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College

Department of Nephrology, West China Hospital, Sichuan University

Department of Medicine Technology, West China School of Public Health, Sichuan University

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