Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress

He-gu Luo; Jia-xu Chen; Qi Zhang; Guang-xin Yue; Jie Ding; Han-ting Zhang; Xian-zhong Yan; Xin Zhao; Zhen-zhi Meng

doi:10.1007/s11655-012-1092-0

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Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress

Updated：2021-08-27

- Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress
- Chinese Journal of Integrative Medicine Vol. 19, Issue 8, Pages: 610-615(2013)
- Affiliations：
  
  1. School of Pre-clinical Medicine, Beijing University of Chinese Medicine,Beijing,China
  2. National Center of Biomedical Analysis,Beijing,China
  3. Department of Behavioral Medicine and Psychiatry, West Virginia University Health Sciences Center, Morgantown,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672578), China National Funds for Distinguished Young Scientists (No. 30825046), and Program for Innovative Research Team in Beijing University of Chinese Medicine (No. 2011CXTD-07)
- DOI：10.1007/s11655-012-1092-0
  CLC：
- Published：2013，
  
  Published Online：19 May 2012，
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Luo, Hg., Chen, Jx., Zhang, Q. et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress., Chin. J. Integr. Med. 19, 610–615 (2013). https://doi.org/10.1007/s11655-012-1092-0

He-gu Luo, Jia-xu Chen, Qi Zhang, et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress. [J]. Chinese Journal of Integrative Medicine 19(8):610-615(2013)
Luo, Hg., Chen, Jx., Zhang, Q. et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress., Chin. J. Integr. Med. 19, 610–615 (2013). https://doi.org/10.1007/s11655-012-1092-0 DOI：

He-gu Luo, Jia-xu Chen, Qi Zhang, et al. Comparative study on effects of Xiaoyao Powder (逍遥散) and its modified prescription on plasma metabolomics of rats with chronic immobilization stress. [J]. Chinese Journal of Integrative Medicine 19(8):610-615(2013) DOI： 10.1007/s11655-012-1092-0.

摘要

To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress (CIS) taking Xiaoyao Powder (逍遥散

XYP) and its modified prescription version

which lacks the volatile oils extracted from Herba Menthae. Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each: control

model

XYP-1 (containing volatile oils from Herba Menthae)

and XYP-2 (lacking volatile oils). All rats except control group rats were subjected to CIS 3 h per day for 21 consecutive days. Groups XYP-1 and XYP-2 were given the extracted XYS with or without volatile oils (3.854 g/kg; suspended in distilled water) via gavage 1 h before CIS each day for 21 days. Rats were anesthetized using intraperitoneal injection of pentobarbital sodium (40 mg/kg) on the 22nd day. Observations were made using a Varian INOVA 600 MHz NMR spectrometer at 27 °C. Carr-Purcell-Meiboom-Gill (CPMG) and longitudinal eddy-delay (LED) were applied

resulting in spectra showing only the signals from micro- and macro-metabolites. Compared to controls

rats subjected to CIS showed increased levels of plasma metabolites

such as acetic acid

choline

N-glycoprotein (NAC)

saturated fatty acid

and blood sugars. Levels of low-density lipoprotein (LDL)

very low-density lipoprotein (VLDL)

and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL

LDL

threonine

methionine

and glutamic acid in plasma. Some common and characteristic metabolites on the anti-CIS of XYP and its modified prescription were obtained. The metabolomics technology is a valuable tool and may be used to identify the specific metabolites and potential biomarkers of therapeutic effect of Chinese medicinal prescriptions.

Abstract

To determine the changes in the levels of endogenous metabolites in rats with chronic immobilization stress (CIS) taking Xiaoyao Powder (逍遥散

XYP) and its modified prescription version

which lacks the volatile oils extracted from Herba Menthae. Twenty-four experimental male SD rats were randomly divided into 4 groups of 6 rats each: control

model

XYP-1 (containing volatile oils from Herba Menthae)

resulting in spectra showing only the signals from micro- and macro-metabolites. Compared to controls

rats subjected to CIS showed increased levels of plasma metabolites

such as acetic acid

choline

N-glycoprotein (NAC)

saturated fatty acid

and blood sugars. Levels of low-density lipoprotein (LDL)

very low-density lipoprotein (VLDL)

and unsaturated fatty acids were decreased. The biochemical effects of XYS were characterized by elevated levels of VLDL

LDL

threonine

methionine

关键词

metabolomicschronic immobilization stressbiomarkerChinese MedicineXiaoyao Powder

Keywords

metabolomicschronic immobilization stressbiomarkerChinese MedicineXiaoyao Powder

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