Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats

Min Ge; Ying-yan Fang; Guo-ping Liu; Su-dong Guan

doi:10.1007/s11655-012-1096-9

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Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats

Updated：2021-08-27

- Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats
- Chinese Journal of Integrative Medicine Vol. 20, Issue 1, Pages: 43-48(2014)
- Affiliations：
  
  Department of Physiology, Bengbu Medical College,Anhui Province,Bengbu,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-012-1096-9
  CLC：
- Published：2014，
  
  Published Online：18 August 2012，
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Ge, M., Fang, Yy., Liu, Gp. et al. Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats., Chin. J. Integr. Med. 20, 43–48 (2014). https://doi.org/10.1007/s11655-012-1096-9

Min Ge, Ying-yan Fang, Guo-ping Liu, et al. Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats. [J]. Chinese Journal of Integrative Medicine 20(1):43-48(2014)
Ge, M., Fang, Yy., Liu, Gp. et al. Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats., Chin. J. Integr. Med. 20, 43–48 (2014). https://doi.org/10.1007/s11655-012-1096-9 DOI：

Min Ge, Ying-yan Fang, Guo-ping Liu, et al. Effect of Shengmai injection (生脉注射液) on diaphragmatic contractility in doxorubicin-treated rats. [J]. Chinese Journal of Integrative Medicine 20(1):43-48(2014) DOI： 10.1007/s11655-012-1096-9.

摘要

To explore the diaphragmatic toxicity in doxorubicin (DOX)-treated rats and the related mechanisms

as well as the effects of Shengmai Injection (SMI

生脉注射液) on the diaphragmatic dysfunction. Thirty Sprague-Dawley male rats were randomly divided into three groups: control

DOX-treated and DOX+SMI treated groups. DOX was given to rats in DOX and DOX+SMI groups in 6 equal doses [2.5 mg/kg

intraperitoneal injection (i.p.)]

on alternate days

over a period of 2 weeks for a cumulative dose of 15 mg/kg. SMI was given to DOX+SMI rats in 12 doses (3 mL/kg

i.p.) for a period of 2 weeks before the administration of DOX and 2 weeks during the administration of DOX. The rats in the control group received equal volume of normal saline. Subsequently

the twitch and tetanic characteristics and force-frequency relationships

and the malondialdehyde (MDA) levels and the superoxide dismutase (SOD) activities

as well as the mRNA content and proteins of inducible nitric oxide synthase (iNOS) were determined. The DOX-treated rats had decreased the peak twitch tension (Pt)

maximal tetanic tension (P0) and force-frequency relationship as compared with the control rats (P<0.01)

while the diaphragm contractility in rats treated with SMI were significantly higher than that in DOX-treated rats (P<0.01). The DOX-treated rats had increased MAD levels and decreased SOD activities (P<0.05)

and SMI decreased the MDA levels and increased the SOD activities in DOX-treated rats (P<0.05). Ultrastructure of diaphragm in the DOX-treated rats revealed typical alterations including fracture of diaphragm fibers

and edema and degeneration of mitochondria; these changes were relieved by SMI treatment. The mRNA content and protein of iNOS in DOX-treated rats were remarkably higher than those in control rats (P<0.01)

while SMI decreased the mRNA expression level of iNOS in DOX-treated rats (P<0.05). Lipid peroxidation is responsible for DOX-induced diaphragm toxicity. SMI protects diaphragm muscles and their function from DOX impairment

and these beneficial effects may be somehow correlated with the decrease in expression of iNOS and lipid peroxidation.

Abstract

To explore the diaphragmatic toxicity in doxorubicin (DOX)-treated rats and the related mechanisms

as well as the effects of Shengmai Injection (SMI

生脉注射液) on the diaphragmatic dysfunction. Thirty Sprague-Dawley male rats were randomly divided into three groups: control

DOX-treated and DOX+SMI treated groups. DOX was given to rats in DOX and DOX+SMI groups in 6 equal doses [2.5 mg/kg

intraperitoneal injection (i.p.)]

on alternate days

over a period of 2 weeks for a cumulative dose of 15 mg/kg. SMI was given to DOX+SMI rats in 12 doses (3 mL/kg

i.p.) for a period of 2 weeks before the administration of DOX and 2 weeks during the administration of DOX. The rats in the control group received equal volume of normal saline. Subsequently

the twitch and tetanic characteristics and force-frequency relationships

and the malondialdehyde (MDA) levels and the superoxide dismutase (SOD) activities

as well as the mRNA content and proteins of inducible nitric oxide synthase (iNOS) were determined. The DOX-treated rats had decreased the peak twitch tension (Pt)

maximal tetanic tension (P0) and force-frequency relationship as compared with the control rats (P<0.01)

and these beneficial effects may be somehow correlated with the decrease in expression of iNOS and lipid peroxidation.

关键词

Shengmai Injectiondoxorubicindiaphragmlipid peroxidationNitric Oxide

Keywords

Shengmai Injectiondoxorubicindiaphragmlipid peroxidationNitric Oxide

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Department of Hematology, the Affiliated Hospital of Guiyang Medical College

Key Laboratory of Basic Pharmacology of Guizhou and Department of Pharmacology, Zunyi Medical College

Tianjin University of Traditional Chinese Medicine

The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine

Institute of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic

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