The absorption and transport of magnolol in Caco-2 cell model

An-guo Wu; Bao Zeng; Meng-qiu Huang; Sheng-mei Li; Jian-nan Chen; Xiao-ping Lai

doi:10.1007/s11655-012-1098-7

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The absorption and transport of magnolol in Caco-2 cell model

Updated：2021-08-27

- The absorption and transport of magnolol in Caco-2 cell model
- Chinese Journal of Integrative Medicine Vol. 19, Issue 3, Pages: 206-211(2013)
- Affiliations：
  
  1. Research Center of New Drug Development, Guangzhou University of Chinese Medicine,Guangzhou,China
  2. Research Institute of Mathematical Engineering of Guangzhou University of Chinese Medicine in Dongguan,Guangdong Province,Dongguan,China
- Author bio：
- Funds：
  
  Supported by the National Key Technology Program (No. 2006BAI11B08-04)
- DOI：10.1007/s11655-012-1098-7
  CLC：
- Published：2013，
  
  Published Online：18 August 2012，
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Wu, Ag., Zeng, B., Huang, Mq. et al. The absorption and transport of magnolol in Caco-2 cell model., Chin. J. Integr. Med. 19, 206–211 (2013). https://doi.org/10.1007/s11655-012-1098-7

An-guo Wu, Bao Zeng, Meng-qiu Huang, et al. The absorption and transport of magnolol in Caco-2 cell model. [J]. Chinese Journal of Integrative Medicine 19(3):206-211(2013)
Wu, Ag., Zeng, B., Huang, Mq. et al. The absorption and transport of magnolol in Caco-2 cell model., Chin. J. Integr. Med. 19, 206–211 (2013). https://doi.org/10.1007/s11655-012-1098-7 DOI：

An-guo Wu, Bao Zeng, Meng-qiu Huang, et al. The absorption and transport of magnolol in Caco-2 cell model. [J]. Chinese Journal of Integrative Medicine 19(3):206-211(2013) DOI： 10.1007/s11655-012-1098-7.

摘要

To investigate the absorption and transport mechanism of magnolol in Caco-2 cell model. A human intestinal epithelial cell model Caco-2 cell in vitro cultured was applied to study the absorption and transport of magnolol

the effects of time

donor concentration

P-gp inhibitor verapamil

pH and temperature on the absorption and transport of magnolol were investigated. The determination of magnolol was performed by high performance liquid chromatography

then the values of apparent permeability coefficient (P app ) and P ratio Basolateral-to-Apical (BL-to-AP)/Apical-to-Basolateral (AP-to-BL) were calculated. In Caco-2 cell model

comparing the amounts of transport of AP-to-BL and BL-to-AP

the latter was larger. At the same donor concentration

either the amounts of transport of AP-to-BL or BL-to-AP increased with increase in donor concentration and incubation time. Verapamil could significantly improve the amounts of transport of AP-to-BL. The transport of AP-to-BL and BL-to-AP depended on temperature

and there was no significant effect of pH on the transport of AP-to-BL. Magnolol could be transported through the intestinal mucosa via a passive diffusion mechanism primarily

coexisting with a carrier-mediated transport

at the same time

the efflux mechanism could be involved.

Abstract

the effects of time

donor concentration

P-gp inhibitor verapamil

pH and temperature on the absorption and transport of magnolol were investigated. The determination of magnolol was performed by high performance liquid chromatography

then the values of apparent permeability coefficient (P app ) and P ratio Basolateral-to-Apical (BL-to-AP)/Apical-to-Basolateral (AP-to-BL) were calculated. In Caco-2 cell model

comparing the amounts of transport of AP-to-BL and BL-to-AP

the latter was larger. At the same donor concentration

and there was no significant effect of pH on the transport of AP-to-BL. Magnolol could be transported through the intestinal mucosa via a passive diffusion mechanism primarily

coexisting with a carrier-mediated transport

at the same time

the efflux mechanism could be involved.

关键词

MagnololCaco-2inhibitorthe apparent permeability coefficientP-gppassive transport

Keywords

MagnololCaco-2inhibitorthe apparent permeability coefficientP-gppassive transport

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