Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats

Yi-cheng Jian; Wei Li; Yi He; Ming Jiang; Yan-bing Liu; Wu-jun Xiong

doi:10.1007/s11655-012-1115-x

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Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats

Updated：2021-08-27

- Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats
- Chinese Journal of Integrative Medicine Vol. 18, Issue 6, Pages: 445-450(2012)
- Affiliations：
  
  Department of Gastroenterology, Shanghai East Hospital Affiliated to Tongji University,Shanghai,China
- Author bio：
- Funds：
  
  Supported by Research Grant for Health Science and Technology of Pudong Health Bureau of Shanghai (No. PKJ2009-Y16)
- DOI：10.1007/s11655-012-1115-x
  CLC：
- Published：2012，
  
  Published Online：22 July 2012，
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Jian, Yc., Li, W., He, Y. et al. Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats., Chin. J. Integr. Med. 18, 445–450 (2012). https://doi.org/10.1007/s11655-012-1115-x

Yi-cheng Jian, Wei Li, Yi He, et al. Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats. [J]. Chinese Journal of Integrative Medicine 18(6):445-450(2012)
Jian, Yc., Li, W., He, Y. et al. Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats., Chin. J. Integr. Med. 18, 445–450 (2012). https://doi.org/10.1007/s11655-012-1115-x DOI：

Yi-cheng Jian, Wei Li, Yi He, et al. Effect of oxymatrine on hepatic gene expression profile in experimental liver fibrosis of rats. [J]. Chinese Journal of Integrative Medicine 18(6):445-450(2012) DOI： 10.1007/s11655-012-1115-x.

摘要

To investigate the effects of oxymatrine on hepatic gene expression profile in a rat model of liver fibrosis. Forty healthy male SD rats were randomly divided into three groups

a normal group (n=8)

a model group (n=16)

and an oxymatrine treatment group (n=16). Experimental hepatic fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4). The rats in the treatment group received oxymatrine via celiac injection at a dosage of 40 mg/kg once a day at the same time. The rats in the model and normal groups received saline at the same dosage via celiac injection. Serum levels of aspartate aminotransferase (AST)

alanine transarninase (ALT)

alkaline phosphatase (AKP)

hyaluronic acid (HA)

and laminin (LN) were assayed. The deposition of collagen was observed with HE and Masson staining. Effect of oxymatrine on hepatic gene expression profile was detected by oligonucleotide microarray analysis with Affymetrix gene chip rat U230A. Quantitative real-time polymerase chain reaction (QRT-PCR) was carried out to confirm the expression changes of six genes. Oxymatrine significantly improved liver function

lowered serum levels of HA and LN

and decreased the degree of liver fibrosis

compared with the model group (P<0.05). A total of 754 differentially expressed genes were identified by gene chip between the model group and the normal group

among which 438 genes increased and 316 genes decreased over two folds. Compared with the model group

86 genes were downregulated markedly in the oxymatrine group (P<0.05)

including collagen I and other genes related to extracellular material (ECM)

integrin signal transduction genes

early growth response factor genes

and proinflammatory genes; 28 genes were upregulated significantly (P<0.05)

including cytochrome P450 (CYP450) superfamily genes

glycolipids metabolism and biological transformation related genes. Six genes were confirmed with QRT-PCR

consistent with the result from microarray. Oxymatrine could affect the expression of many functional genes and may be useful in the prevention and treatment of liver fibrosis.

Abstract

To investigate the effects of oxymatrine on hepatic gene expression profile in a rat model of liver fibrosis. Forty healthy male SD rats were randomly divided into three groups

a normal group (n=8)

a model group (n=16)

alanine transarninase (ALT)

alkaline phosphatase (AKP)

hyaluronic acid (HA)

lowered serum levels of HA and LN

and decreased the degree of liver fibrosis

compared with the model group (P<0.05). A total of 754 differentially expressed genes were identified by gene chip between the model group and the normal group

among which 438 genes increased and 316 genes decreased over two folds. Compared with the model group

86 genes were downregulated markedly in the oxymatrine group (P<0.05)

including collagen I and other genes related to extracellular material (ECM)

integrin signal transduction genes

early growth response factor genes

and proinflammatory genes; 28 genes were upregulated significantly (P<0.05)

including cytochrome P450 (CYP450) superfamily genes

glycolipids metabolism and biological transformation related genes. Six genes were confirmed with QRT-PCR

consistent with the result from microarray. Oxymatrine could affect the expression of many functional genes and may be useful in the prevention and treatment of liver fibrosis.

关键词

Oxymatrinegene chipLiver Fibrosis

Keywords

Oxymatrinegene chipLiver Fibrosis

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Related Institution

Key Laboratory of Liver and Kidney Diseases, Shanghai University of Traditional Chinese Medicine

Institute of Traditional Chinese Internal Medicine, Shanghai Municipal Education Commission, Shanghai

Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Institute of Liver Diseases, Shanghai University of Chinese medicine

Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Tecnology

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