Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro

A.-ling Shen; Fei Hong; Li-ya Liu; Jiu-mao Lin; Qun-chuan Zhuang; Zhen-feng Hong; Jun Peng

doi:10.1007/s11655-012-1121-z

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Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro

Updated：2021-08-27

- Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro
- Chinese Journal of Integrative Medicine Vol. 18, Issue 6, Pages: 431-436(2012)
- Affiliations：
  
  1. Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Postdoctor Workstation, Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd.,Fujian,China
- Author bio：
- Funds：
  
  Surpported by the National Natural Science Foundation of China (No. 81073097), the Developmental Fund of Chen Ke-ji Integrative Medicine (No. CKJ 2011001), and the Natural Science Foundation of Fujian Province of China (No. 2010J01195)
- DOI：10.1007/s11655-012-1121-z
  CLC：
- Published：2012，
  
  Published Online：22 July 2012，
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Shen, Al., Hong, F., Liu, Ly. et al. Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro, ., Chin. J. Integr. Med. 18, 431–436 (2012). https://doi.org/10.1007/s11655-012-1121-z

A.-ling Shen, Fei Hong, Li-ya Liu, et al. Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro. [J]. Chinese Journal of Integrative Medicine 18(6):431-436(2012)
Shen, Al., Hong, F., Liu, Ly. et al. Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro, ., Chin. J. Integr. Med. 18, 431–436 (2012). https://doi.org/10.1007/s11655-012-1121-z DOI：

A.-ling Shen, Fei Hong, Li-ya Liu, et al. Effects of Pien Tze Huang (片仔癀) on angiogenesis in vivo and in vitro. [J]. Chinese Journal of Integrative Medicine 18(6):431-436(2012) DOI： 10.1007/s11655-012-1121-z.

摘要

To investigate the anti-angiogenic effects of Pien Tze Huang (片仔癀

PZH) in vivo and in vitro. Human umbilical vein endothelial cells (HUVECs) were treated with 0 mg/mL

0.25 mg/mL

0.5 mg/mL

and 1 mg/mL of PZH for 24 h

48 h and 72 h

respectively. Chicken embryo chorioallantoic membrane (CAM) model was used to evaluate in vivo angiogenesis. An ECMatrix gel system was used to evaluate in vitro angiogenesis by examining the tube formation of HUVECs. 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide assay was performed to determine HUVEC viability. Cell density of HUVECs was observed by phasecontrast microscopy. HUVEC migration was determined by wound healing method. The mRNA and protein expression of vascular endothelial growth factor A (VEGF-A) and basic fibroblast growth factor (bFGF) in both HUVEC and human colon adenocarcinoma cells (HT-29) was examined by reverse transcription polymerase chain reaction (RT-PCR) and enzyme linked immune sorbent assay (ELISA)

respectively. PZH treatment significantly reduced the total number of blood vessels compared with the untreated control in the chicken embryos and resulted in a significant decrease in capillary tube formation and cell density of HUVECs (P<0.05). In addition

treatment with 0.25–1 mg/mL of PZH for 24 h

48 h

and 72 h respectively reduced cell viability by 9%–52%

24%–87% or 25%–87%

compared with the untreated control cells (P<0.05). Moreover

PZH treatment decreased the migration of HUVECs. Furthermore

PZH dose-dependently suppressed the expression of VEGF-A and bFGF on both mRNA and protein levels (P<0.05). PZH could inhibit angiogenesis in vivo in CAM model and in vitro on HUVECs

suggesting that inhibiting tumor angiogenesis might be one of the mechanisms by which PZH treats cancer.

Abstract

To investigate the anti-angiogenic effects of Pien Tze Huang (片仔癀

PZH) in vivo and in vitro. Human umbilical vein endothelial cells (HUVECs) were treated with 0 mg/mL

0.25 mg/mL

0.5 mg/mL

and 1 mg/mL of PZH for 24 h

48 h and 72 h

5-dimethylthiazol-2-yl)-2

treatment with 0.25–1 mg/mL of PZH for 24 h

48 h

and 72 h respectively reduced cell viability by 9%–52%

24%–87% or 25%–87%

compared with the untreated control cells (P<0.05). Moreover

PZH treatment decreased the migration of HUVECs. Furthermore

PZH dose-dependently suppressed the expression of VEGF-A and bFGF on both mRNA and protein levels (P<0.05). PZH could inhibit angiogenesis in vivo in CAM model and in vitro on HUVECs

suggesting that inhibiting tumor angiogenesis might be one of the mechanisms by which PZH treats cancer.

关键词

Pien Tze Huangtumor angiogenesisChinese Medicinemulti-target agents

Keywords

Pien Tze Huangtumor angiogenesisChinese Medicinemulti-target agents

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Related Institution

Fujian Academy of Integrative Medicine

Department of Pediatrics, The University of Oklahoma Heatth Sciences Center, Oklahoma, City

Department of Biochemistry and Molecular Biology, The University of Oklahoma Health Sciences Center

Georg-August-Universität Göttingen, Göttingen

The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics’ Ministry of Public Health

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