Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice

Wei Jiang; Song Li; Wei Mao; Guang Yang; Xin-mei Li; Guang-juan Zheng; Huan-lin Wu; Xin-min Ruan; Ke-ji Chen

doi:10.1007/s11655-012-1123-x

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Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice

Updated：2021-08-27

- Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice
- Chinese Journal of Integrative Medicine Vol. 18, Issue 6, Pages: 451-456(2012)
- Affiliations：
  
  1. The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
  2. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81102584) and Guangdong Provincial Administration of Traditional Chinese Medicine (No. 2009386)
- DOI：10.1007/s11655-012-1123-x
  CLC：
- Published：2012，
  
  Published Online：22 July 2012，
Scan for full text
Jiang, W., Li, S., Mao, W. et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice., Chin. J. Integr. Med. 18, 451–456 (2012). https://doi.org/10.1007/s11655-012-1123-x

Wei Jiang, Song Li, Wei Mao, et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice. [J]. Chinese Journal of Integrative Medicine 18(6):451-456(2012)
Jiang, W., Li, S., Mao, W. et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice., Chin. J. Integr. Med. 18, 451–456 (2012). https://doi.org/10.1007/s11655-012-1123-x DOI：

Wei Jiang, Song Li, Wei Mao, et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice. [J]. Chinese Journal of Integrative Medicine 18(6):451-456(2012) DOI： 10.1007/s11655-012-1123-x.

摘要

To observe the effect of Huxin Formula (护心方

HXF) on expressions of the chief reverse cholesterol transport (RCT) associated genes

caveolin-1 and scavenger receptor-BI (SR-BI) in ApoE-gene knockout [ApoE (−/−)] mice. Thirty ApoE (−/−) mice of 4–6 weeks old were randomly divided into three groups (A-C). After being fed with high-fat diet for 16 weeks

they were treated with HXF (1 mL/100 g)

pravachol (0.3 mg/100 g)

and saline in equal volume respectively for 16 weeks successively; in addition

a blank group was set up with 10 C57BL/6J mice of 6-week old received 16-week high-fat feeding and saline treatment. Animals were sacrificed at the termination of the experiment

their paraffin sections of aortic tissue were used to measure the size of plaque

expressions of cavolin-1 and SR-BI were detected by immunological histochemical method. As compared with the blank group

levels of caveolin-1 and SR-BI were increased in Groups A and B (P<0.01); but the increase in Group A was more significant than that in Group B (P<0.05). The plaque/aorta area ratio decreased significantly in Groups A and B

but showed insignificant difference between the two groups. HXF could obviously increase the expressions of RCT associated genes

caveolin-1 and SR-BI

promote the RCT process

so as to reduce the formation of aorta atherosclerotic plaque in ApoE (−/−) mice.

Abstract

To observe the effect of Huxin Formula (护心方

HXF) on expressions of the chief reverse cholesterol transport (RCT) associated genes

they were treated with HXF (1 mL/100 g)

pravachol (0.3 mg/100 g)

and saline in equal volume respectively for 16 weeks successively; in addition

a blank group was set up with 10 C57BL/6J mice of 6-week old received 16-week high-fat feeding and saline treatment. Animals were sacrificed at the termination of the experiment

their paraffin sections of aortic tissue were used to measure the size of plaque

expressions of cavolin-1 and SR-BI were detected by immunological histochemical method. As compared with the blank group

but showed insignificant difference between the two groups. HXF could obviously increase the expressions of RCT associated genes

caveolin-1 and SR-BI

promote the RCT process

so as to reduce the formation of aorta atherosclerotic plaque in ApoE (−/−) mice.

关键词

Huxin formulaApoE-gene knockout micereverse cholesterol transportcaveolin-1scavenger receptor-B I

Keywords

Huxin formulaApoE-gene knockout micereverse cholesterol transportcaveolin-1scavenger receptor-B I

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