A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells

Jun Song; Wen-yuan Chen; Li-ya Wu; Liang-pu Zheng; Wei Lin; Dong Gao; Ted J. Kaptchuk; Ke-ji Chen

doi:10.1007/s11655-012-1143-6

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A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells

Updated：2021-08-27

- A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells
- Chinese Journal of Integrative Medicine Vol. 18, Issue 7, Pages: 502-506(2012)
- Affiliations：
  
  1. Experimental Research Center, China Academy of Chinese Medical Sciences,Beijing,China
  2. Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Fujian Academy of Integrative Medicine,Fuzhou,China
  4. Harvard Medical School,Boston,USA
  5. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072933 and No. 81173431);Fujian Natural Science Foundation (No. 2007J0101);Fujian Academy of Integrative Medicine Foundation (No. CKJ2008001);National Institutes of Health—The National Center for Complementary and Alternative Medicine Foundation of US (NIH-NCCAM No. K24 AT004095)
- DOI：10.1007/s11655-012-1143-6
  CLC：
- Published：2012，
  
  Published Online：27 March 2013，
Scan for full text
Song, J., Chen, Wy., Wu, Ly. et al. A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells., Chin. J. Integr. Med. 18, 502–506 (2012). https://doi.org/10.1007/s11655-012-1143-6

Jun Song, Wen-yuan Chen, Li-ya Wu, et al. A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells. [J]. Chinese Journal of Integrative Medicine 18(7):502-506(2012)
Song, J., Chen, Wy., Wu, Ly. et al. A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells., Chin. J. Integr. Med. 18, 502–506 (2012). https://doi.org/10.1007/s11655-012-1143-6 DOI：

Jun Song, Wen-yuan Chen, Li-ya Wu, et al. A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells. [J]. Chinese Journal of Integrative Medicine 18(7):502-506(2012) DOI： 10.1007/s11655-012-1143-6.

摘要

To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction (血府逐瘀汤) on the endothelial cell line ECV304. ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoctioncontaining serum (XFZYD-CS) for 24 h

48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT)

fluorescence activating cell sorter (FACS)

migration

adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction (RT-PCR) superarray. At 48 h

XFZYD-CS induced ECV304 significantly improved cell viability

number in S phase

migration

adhesion and tube formation. At 24 h and 72 h

only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed. XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.

Abstract

48 h or 72 h. Thiazolyl blue tetrazolium bromide (MTT)

fluorescence activating cell sorter (FACS)

migration

XFZYD-CS induced ECV304 significantly improved cell viability

number in S phase

migration

adhesion and tube formation. At 24 h and 72 h

关键词

Xuefu Zhuyu DecoctionangiogenesismicroarrayChinese herbs

Keywords

Xuefu Zhuyu DecoctionangiogenesismicroarrayChinese herbs

references

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