Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells

Chun-yan Feng; Xiu-rong Huang; Ming-xin Qi; Song-wen Tang; Yan-hong Hu; Sheng Chen; Fa-jie Ke

doi:10.1007/s11655-012-1144-5

Your Location：

Home >

Browse articles >

Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells

Updated：2021-08-27

- Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells
- Chinese Journal of Integrative Medicine Vol. 18, Issue 7, Pages: 529-533(2012)
- Affiliations：
  
  1. Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Foundation of Fujian Province Health Department Fund (No.2009-1-30) and Fujian Provincie Department of Education Issues (No. JA10176)
- DOI：10.1007/s11655-012-1144-5
  CLC：
- Published：2012，
  
  Published Online：27 March 2013，
Scan for full text
Feng, Cy., Huang, Xr., Qi, Mx. et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells., Chin. J. Integr. Med. 18, 529–533 (2012). https://doi.org/10.1007/s11655-012-1144-5

Chun-yan Feng, Xiu-rong Huang, Ming-xin Qi, et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells. [J]. Chinese Journal of Integrative Medicine 18(7):529-533(2012)
Feng, Cy., Huang, Xr., Qi, Mx. et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells., Chin. J. Integr. Med. 18, 529–533 (2012). https://doi.org/10.1007/s11655-012-1144-5 DOI：

Chun-yan Feng, Xiu-rong Huang, Ming-xin Qi, et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells. [J]. Chinese Journal of Integrative Medicine 18(7):529-533(2012) DOI： 10.1007/s11655-012-1144-5.

摘要

To investigate the protective effects of the natural medicinal monomer isopsoralen (ISR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide (H2O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. HLE-B3 cells were treated with H2O2 (300 μ mol/L)

β-estradiol (E2: 10−8 mol/L) and H2O2

ISR (10−5 mol/L) and H2O2

or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surfaceenhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (m/z) ratios of each peak were tested by the Kruskal-Wallis rank sum test

and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. H2O2 up-regulated the expressions of two protein spots (with m/z of 6532 and 6809). E2 mitigated the oxidative damage

and the expression of one protein spot (m/z 6532) was down-regulated. In contrast

ISR down-regulated both of protein spots (m/z 6532 and 6809). ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.

Abstract

β-estradiol (E2: 10−8 mol/L) and H2O2

ISR (10−5 mol/L) and H2O2

and the expression of one protein spot (m/z 6532) was down-regulated. In contrast

关键词

isopsoralenmitochondrial proteomicslens epithelial cellsenile cataract

Keywords

isopsoralenmitochondrial proteomicslens epithelial cellsenile cataract

references

Klein BE, Klein R, Linton KL. Prevalence of age related lens opacities in a population. The Beaver Dam Eye Study. Ophthalmol 1992;99:546–552.

Cuming RG, Mitchell P. Hormone replacement therapy, reproductive factors, and cataract. The Bluc Mountains Eye Study. Am J Epidemiol 1997;145:242–249.

Dolatowska E. The evaluation of estradiol and FSH serum levels in menopausal women with primary cataract. Klin Oczna 2002;104:357–361.

Wang X, Simpkins JW, Dykens JA, Cammarata PR. Oxidative damage to human lens epithelial cells in culture: estrogen protection of mitochondrial potential, ATP, and cell viability. Invest Ophthalmol Vis Sci 2003;44:2067–2075.

Moor AN, Flynn JM, Gottipati S, Giblin FJ, Cammarata PR. 17beta-estradiol stimulates MAPK signaling pathway in human lens epithelial cell cultures preventing collapse of mitochondrial membrane potential during acute oxidative stress. Mitochondrion 2005;5:235–247.

Moor AN, Gottipati S, Mallet RT, Sun J, Giblin FJ, Roque R, Cammarata PR. A putative mitochondrial mechanism for antioxidative cytoprotection by 17 betaestradiol. Exp Eye Res 2004;78:933–944.

Flynn JM, Cammarata PR. Estradiol attenuates mitochondrial depolarization in polyol-stressed lens epithelial cells. Mol Vis 2006;12:271–282.

Gottipati S, Cammarata PR. Mitochondrial super-oxide dismutase activation with 17 β-estradiol-treated human lens epithelial cells. Mol Vis 2008;14:898–905.

Zhao PW, Wang DW, Wang LQ. Expression study in screening estrogen-like ability of ten traditional Chinese drugs using uterus increased weight. J Beijing Univ Tradit Chin Med (Chin) 2006;29:686–689.

Zhao PW, Niu JZ, Wang JF. Influence of Isopsoralen and ecdysterone in proliferation of human breast carcinoma cell. J Beijing Univ Tradit Chin Med (Chin) 2007;30:242–245.

Qi MX, Huang XR, Zhang KL, Guo N. Isopsoralen protecting against apoptosis of human lens epithelial cell. Chin J Clin Pharmacol Ther (Chin) 2009;14:1371–1374.

Huang X, Qi M, Zhang K, Guo N. Upregulatory effect of isopsoralen on expression of estrogen receptor in human lens epithelial cell. Chin Pathophysioloy (Chin) 2010;26:1844–1848.

Feng CY, Huang XR, Qi MX, Tang SW, Guo N, Hu YH. The protective effects and mechanisms of Isopsoralen in oxidative damage of human lens epithelial cell. China J Ophthalmol (Chin) 2011;47:353–355.

Hu YH, Huang XR, Qi MX, Hou BY. Study on proteomics of inhibitory effects of elemene on proliferation of human lens epithelial cell. China J Ophthalmol (Chin) 2010;46:427–431.

Nordgaard CL, Karunadharma PP, Feng X, Olsen TW, Ferrington DA. Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration. Invest Ophthalmol Vis Sci 2008;49:2848–2855.

Saraswathy S, Rao NA. Mitochondrial proteomics in experimental autoimmune uveitis oxidative stress. Invest Ophthalmol Vis Sci 2009;50:5559–5566.

Zhang Q, Liu Y, Ren YF. Detection of distinct protein in renal cell carcinoma cell line and normal kidney cell line by SELDI-TOFMS technology. Applied J Gen Pract (Chin) 2008;6:333–334.

Ge Z, Zhu YL, Zhong X. The influence of Helicobacter pylori associated protein A in human gastric adenocarcinoma epithelium cell proteome. Cell Biology 2006;28:603–610.

FULL TEXT LINK

More>

Views

837

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Casticin Attenuates Stemness in Cervical Cancer Stem-Like Cells by Regulating Activity and Expression of DNMT1

Radix Scrophulariae Extracts Exert Effect on Hyperthyroidism via MST1/Hippo Signaling Pathway

Related Author

No data

Related Institution

Medical College, Hunan University of Medicine, Huaihua, Hunan Province, China;Clinical Department of Guangdong Metabolic Disease Research Centre of Integrated Chinese and Western Medicine, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China;Institute of Chemical Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China;The Key Laboratory of Model Animals and Stem Cell Biology in Hunan Province, Medical College, Hunan Normal University, Changsha, China;The Key Laboratory of Study and Discover of Small Targeted Molecules of Hunan Province, Medical College, Hunan Normal University, Changsha, China

Department of Pharmacy, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, China;Department of Chinese Medicine Rehabilitation, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, China;College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, China;College of Life and Health, Dalian University, Dalian, Liaoning Province, China

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰