Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro

Ling-yun Zhang; Min Ou; You-zhang Huang; Yuan-yuan Qiao; Da-jin Zhang

doi:10.1007/s11655-012-1150-7

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Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro

Updated：2021-08-27

- Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro
- Chinese Journal of Integrative Medicine Vol. 18, Issue 7, Pages: 507-513(2012)
- Affiliations：
  
  1. Institute of Very Important Person Pulmonary Medicine, Navy General Hospital of People’s Liberation Army,Beijing,China
  2. Institute of Hematology, Navy General Hospital of People’s Liberation Army,Beijing,China
  3. Institute of Central Laboratory, Navy General Hospital of People’s Liberation Army,Beijing,China
- Author bio：
- Funds：
  
  Supported by Military Medical and Health Foundation (No. 36040)
- DOI：10.1007/s11655-012-1150-7
  CLC：
- Published：2012，
  
  Published Online：27 March 2013，
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Zhang, Ly., Ou, M., Huang, Yz. et al. Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro, ., Chin. J. Integr. Med. 18, 507–513 (2012). https://doi.org/10.1007/s11655-012-1150-7

Ling-yun Zhang, Min Ou, You-zhang Huang, et al. Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro. [J]. Chinese Journal of Integrative Medicine 18(7):507-513(2012)
Zhang, Ly., Ou, M., Huang, Yz. et al. Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro, ., Chin. J. Integr. Med. 18, 507–513 (2012). https://doi.org/10.1007/s11655-012-1150-7 DOI：

Ling-yun Zhang, Min Ou, You-zhang Huang, et al. Effect of Yifei Huoxue Granule (益肺活血颗粒) on the proliferation of rat pulmonary artery smooth muscle cells upon exposure to chronic hypoxic conditions in vitro. [J]. Chinese Journal of Integrative Medicine 18(7):507-513(2012) DOI： 10.1007/s11655-012-1150-7.

摘要

To investigate the inhibitory effect of Yifei Huoxue Granule (益肺活血颗粒

YFHXG): on the hypoxia-induced proliferation of rat pulmonary artery smooth muscle cells (PASMCs) and its mechanism of decreasing pulmonary arterial pressure. Twenty male Sprague-Dawley (SD) rats were randomly: divided into four groups: saline

and 0.66

3.30 and 16.50 g/kg of YFHXG groups

the saline and different concentrations of YFHXG were given twice daily for 7 days

respectively. Serum-pharmacology method was used in the preparation of YFHXG serum. Tissue block anchorage was employed in the primary culture of rat PASMCs. The PASMCs were randomly divided into normoxia group

hypoxia group

and hypoxia+YFHXG group (0.66

3.30 and 16.50 g/kg doses of YFHXG-treated serum groups

exposed to hypoxic condition). PASMCs in normoxia and hypoxia group were cultured with saline serum

hypoxia+YFHXG groups were cultured with different concentrations of YFHXG serum. Cell viability was assessed with 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Cell cycle was analyzed using flow cytometry. In addition

hypoxia inducible factor-1-alpha (HIF-1α) protein expression was evaluated by immunocytochemistry analysis

the concentration of intracellular reactive oxygen species (ROS) and Ca Ca2+ were determined by laser scanning confocal microscopy (LSCM). MTT assay and flow cytometry showed that hypoxia could directly activate the proliferation of PASMCs

while YFHXG dose-dependently inhibited hypoxia-induced proliferation of rat PASMCs. Immunocytochemistry showed that hypoxia enhanced HIF-1α protein expression

and LSCM showed that hypoxia significantly increased intracellular ROS and Ca Ca2+

while YFHXG decreased the expression of HIF-

1α and attenuated the hypoxia-induced increase in intracellular concentration of ROS and Ca Ca2+. YFHXG could inhibit hypoxia-induced proliferation of rat PASMCs

which may decrease pulmonary arterial pressure and vascular remodeling. The anti-hypoxia effect of YFHXG may be explained by its regulation of HIF-1α expression and of the levels of intracellular ROS and Ca Ca2+.

Abstract

To investigate the inhibitory effect of Yifei Huoxue Granule (益肺活血颗粒

and 0.66

3.30 and 16.50 g/kg of YFHXG groups

the saline and different concentrations of YFHXG were given twice daily for 7 days

hypoxia group

and hypoxia+YFHXG group (0.66

3.30 and 16.50 g/kg doses of YFHXG-treated serum groups

exposed to hypoxic condition). PASMCs in normoxia and hypoxia group were cultured with saline serum

hypoxia+YFHXG groups were cultured with different concentrations of YFHXG serum. Cell viability was assessed with 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Cell cycle was analyzed using flow cytometry. In addition

hypoxia inducible factor-1-alpha (HIF-1α) protein expression was evaluated by immunocytochemistry analysis

while YFHXG dose-dependently inhibited hypoxia-induced proliferation of rat PASMCs. Immunocytochemistry showed that hypoxia enhanced HIF-1α protein expression

and LSCM showed that hypoxia significantly increased intracellular ROS and Ca Ca2+

while YFHXG decreased the expression of HIF-

1α and attenuated the hypoxia-induced increase in intracellular concentration of ROS and Ca Ca2+. YFHXG could inhibit hypoxia-induced proliferation of rat PASMCs

关键词

Yifei Huoxue Granulehypoxiapulmonary artery smooth muscle cellsproliferationhypoxia inducible factor-1αreactive oxygen species

Keywords

Yifei Huoxue Granulehypoxiapulmonary artery smooth muscle cellsproliferationhypoxia inducible factor-1αreactive oxygen species

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