Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract

Bi-ying Xiao; Shu-jie Mao; Xian-duan Li

doi:10.1007/s11655-012-1178-8

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Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract

Updated：2021-08-27

- Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract
- Chinese Journal of Integrative Medicine Vol. 18, Issue 10, Pages: 782-787(2012)
- Affiliations：
  
  1. Jiangxi University of Traditional Chinese Medicine,Nanchang,China
  2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Innovation Group Project of China Academy of Chinese Medical Sciences (No. ZZ2006096)
- DOI：10.1007/s11655-012-1178-8
  CLC：
- Published：2012，
  
  Published Online：11 September 2012，
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Xiao, By., Mao, Sj. & Li, Xd. Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract., Chin. J. Integr. Med. 18, 782–787 (2012). https://doi.org/10.1007/s11655-012-1178-8

Bi-ying Xiao, Shu-jie Mao, Xian-duan Li. Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract. [J]. Chinese Journal of Integrative Medicine 18(10):782-787(2012)
Xiao, By., Mao, Sj. & Li, Xd. Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract., Chin. J. Integr. Med. 18, 782–787 (2012). https://doi.org/10.1007/s11655-012-1178-8 DOI：

Bi-ying Xiao, Shu-jie Mao, Xian-duan Li. Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract. [J]. Chinese Journal of Integrative Medicine 18(10):782-787(2012) DOI： 10.1007/s11655-012-1178-8.

摘要

To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine

including evodiamine and glycyrrhizic acid

during processing of Fructus Evodiae with Radix Glycyrrhizae extract by using high performance liquid chromatography (HPLC) and to provide a scientific basis for different clinical uses of processed and unprocessed Fructus Evodiae. The concentrations of the Fructus Evodiae components in processed or unprocessed Fructus Evodiae were evaluated by HPLC using a YMC J’sphere ODS-H80 column (4.6 mm×250 mm

5 μm) with acetonitrile-water-tetrahydrofuran-acetic acid (41:59:1:0.2

v/v/v/v) as the mobile phase. The detection wavelength was 225 nm

the column temperature was 35°C

the flow rate was 1.0 mL/min

and the injection volume was 10 μL. The concentrations of the Radix Glycyrrhizae components were determined by HPLC with a Kromasil-C18 column (4.6 mm×250 mm

4 μm) and a gradient elution of acetonitrile (A) and 0.05% aqueous phosphoric acid (B) as the mobile phase. The detection wavelength was 237 nm

the column temperature was 35 °C

the flow rate was 1.0 mL/min

and the injection volume was 10 μL. The calibration curves of evodia lactone

evodiamine

rutaecarpine

liquiritin

and glycyrrhizin showed good linear relationships (r>0.99). The recoveries of evodia lactone

evodiamine

rutaecarpine

liquiritin

and glycyrrhizin were 96.59%

104.18%

101.91%

97.75%

and 97.95%

respectively. The concentrations of the components in processed Fructus Evodiae were obviously different to those in unprocessed Fructus Evodiae. The developed method is rapid and accurate. The results provide a reference for processed Fructus Evodiae and the changes that could be expected in its effects compared to unprocessed Fructus Evodiae

Abstract

To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine

including evodiamine and glycyrrhizic acid

5 μm) with acetonitrile-water-tetrahydrofuran-acetic acid (41:59:1:0.2

v/v/v/v) as the mobile phase. The detection wavelength was 225 nm

the column temperature was 35°C

the flow rate was 1.0 mL/min

and the injection volume was 10 μL. The concentrations of the Radix Glycyrrhizae components were determined by HPLC with a Kromasil-C18 column (4.6 mm×250 mm

4 μm) and a gradient elution of acetonitrile (A) and 0.05% aqueous phosphoric acid (B) as the mobile phase. The detection wavelength was 237 nm

the column temperature was 35 °C

the flow rate was 1.0 mL/min

and the injection volume was 10 μL. The calibration curves of evodia lactone

evodiamine

rutaecarpine

liquiritin

and glycyrrhizin showed good linear relationships (r>0.99). The recoveries of evodia lactone

evodiamine

rutaecarpine

liquiritin

and glycyrrhizin were 96.59%

104.18%

101.91%

97.75%

and 97.95%

关键词

Radix GlycyrrhizaeFructus EvodiaeHigh Performance Liquid Chromatography

Keywords

Radix GlycyrrhizaeFructus EvodiaeHigh Performance Liquid Chromatography

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