Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells

Li-ming Yin; Xiao Wang; Xu-dai Qian; Xiao-jie Lin; Xiao-hong Chen; Rui-lan Gao

doi:10.1007/s11655-012-1179-7

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Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells

Updated：2021-08-27

- Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells
- Chinese Journal of Integrative Medicine Vol. 18, Issue 8, Pages: 616-620(2012)
- Affiliations：
  
  Institute of Hematology, the First Hospital Affiliated to Zhejiang University of Chinese Medicine,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the Grant from Zhejiang Provincial Natural Science Foundation of China (No. Y207728), and Traditional Chinese Medicine Adiministration Bureau of Zhejiang Province, China (No. 2008YA005)
- DOI：10.1007/s11655-012-1179-7
  CLC：
- Published：2012，
  
  Published Online：2 August 2012，
Scan for full text
Yin, Lm., Wang, X., Qian, Xd. et al. Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells., Chin. J. Integr. Med. 18, 616–620 (2012). https://doi.org/10.1007/s11655-012-1179-7

Li-ming Yin, Xiao Wang, Xu-dai Qian, et al. Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells. [J]. Chinese Journal of Integrative Medicine 18(8):616-620(2012)
Yin, Lm., Wang, X., Qian, Xd. et al. Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells., Chin. J. Integr. Med. 18, 616–620 (2012). https://doi.org/10.1007/s11655-012-1179-7 DOI：

Li-ming Yin, Xiao Wang, Xu-dai Qian, et al. Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells. [J]. Chinese Journal of Integrative Medicine 18(8):616-620(2012) DOI： 10.1007/s11655-012-1179-7.

摘要

To investigate the effects of Panax notoginseng saponins (PNS) on the proliferation and differentiation in NIH3T3 cells. NIH3T3 cells were treated by various concentrations of PNS 0

0.05

0.10

0.20

and 0.40 g/L. The vitality and proliferation potential of cells were detected by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay

the alkaline phosphatase (ALP) activity was measured by p-nitrophenyl phosphate (pNPP) assay

and the mineralization formation ability was tested for the cellular differentiation toward osteoblast

as well as the expression level of phosphorylated extracellular signal-regulated kinase1/2(P-ERK1/2)

extracellular signal-regulated kinase1/2 (ERK1/2) protein kinase was analyzed by Western blot with total cell lysate of NIH3T3 cells treated by PNS. Both MTT and pNPP assay showed that optical density (OD) values were increased in response to PNS treatment at a dose-dependent pattern. The mineralization formation ability was enhanced in PNS-treated NIH3T3 cells compared with untreated cells. Meanwhile

the expression level of P-ERK1/2 protein kinase was up-regulated in PNS-treated NIH3T3 cells

while

the expression level of ERK1/2 protein kinase revealed no obvious difference with or without PNS treated cells. PNS could pay a role to promote the proliferation and differentiation in NIH3T3 cells by means of up-regulation of P-ERK1/2 protein kinase.

Abstract

To investigate the effects of Panax notoginseng saponins (PNS) on the proliferation and differentiation in NIH3T3 cells. NIH3T3 cells were treated by various concentrations of PNS 0

0.05

0.10

0.20

and 0.40 g/L. The vitality and proliferation potential of cells were detected by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay

the alkaline phosphatase (ALP) activity was measured by p-nitrophenyl phosphate (pNPP) assay

and the mineralization formation ability was tested for the cellular differentiation toward osteoblast

as well as the expression level of phosphorylated extracellular signal-regulated kinase1/2(P-ERK1/2)

the expression level of P-ERK1/2 protein kinase was up-regulated in PNS-treated NIH3T3 cells

while

关键词

panax notoginseng saponinsNIH3T3 cellsosteoblastextracellular signal-regulated kinase protein kinase

Keywords

panax notoginseng saponinsNIH3T3 cellsosteoblastextracellular signal-regulated kinase protein kinase

references

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Institution of Hematology Research, The First Affiliated Hospital, Zhejiang Chinese Medical University

Department of Oncology, Zhejiang Provincial People’s Hospital

Department of Orthopedics, the Second Affiliated Hospital of Medical College of Xi’an Jiaotong University

Department of Orthopedics, the Second Hospital of Tsinghua University

Department of Orthopedics, Shaanxi Provincial People’s Hospital

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