Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer

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Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer

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- Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer
- Chinese Journal of Integrative Medicine Vol. 18, Issue 8, Pages: 605-609(2012)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine Oncology, Provincial Hospital of Anhui Medical University,Hefei,China
  2. Department of Oncology, Affiliated Hospital of Bengbu Medical College,Anhui Province,Bengbu,China
- Author bio：
- Funds：
  
  Supported by the State Administration of Traditional Chinese Medicine of China (2009-No.30)
- DOI：10.1007/s11655-012-1184-x
  CLC：
- Published：2012，
  
  Published Online：2 August 2012，
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Li, P., Zhang, M., Ma, Wj. et al. Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer., Chin. J. Integr. Med. 18, 605–609 (2012). https://doi.org/10.1007/s11655-012-1184-x

Ping Li, Mei Zhang, Wen-jing Ma, et al. Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer. [J]. Chinese Journal of Integrative Medicine 18(8):605-609(2012)
Li, P., Zhang, M., Ma, Wj. et al. Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer., Chin. J. Integr. Med. 18, 605–609 (2012). https://doi.org/10.1007/s11655-012-1184-x DOI：

Ping Li, Mei Zhang, Wen-jing Ma, et al. Effects of brucine on vascular endothelial growth factor expression and microvessel density in a nude mouse model of bone metastasis due to breast cancer. [J]. Chinese Journal of Integrative Medicine 18(8):605-609(2012) DOI： 10.1007/s11655-012-1184-x.

摘要

To study the effects of brucine on vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in a nude mouse model of bone metastasis due to breast cancer

and to assess the possible antitumor mechanism of brucine. A syringe needle was used to directly inject 0.2 mL monoplast suspension (with 2×105 human breast cancer cells contained) into the bony femoral cortex of the right hind leg for modeling. Twenty-five nude mice were randomized into five groups and administered with an intraperitoneal injection of saline or drug for 8 consecutive days: model group (0.2 mL normal saline)

low-dose brucine group (1.73 mg·kg−1)

medium-dose brucine group (3.45 mg·kg−1)

high-dose brucine group (6.90 mg·kg−1)

and thalidomide group (200 mg·kg−1). Diet and activity were recorded

and the tumors were harvested 5 weeks later. The percentage of VEGF-positive cells was determined with hematoxylin and eosin staining and immunohistochemical staining

and MVD expression was determined by optical microscopy. The VEGF expressions in brucine- or thalidomide-treated mice were significantly reduced as compared with mice in the model group (P <0.01). There were no significant difference between the high-dose brucine group and the thalidomide group (P >0.05). Significant difference was between the high- and low-dose brucine group P<0.05). Further

VEGF expression was significantly increased in the low- and medium-dose brucine groups compared with the thalidomide group (P <0.05). The MVD values in the three brucine and thalidomide groups were significantly lower than that in the model group (P <0.01). The MVD values in the medium- and high-dose brucine groups were not significantly different from those in the thalidomide group (P >0.05)

while the MVD value showed a significant increase in the low-dose group compared with the thalidomide group (P <0.05). Brucine could inhibit the growth of breast cancer to bone metastases

possibly by inhibiting tumor angiogenesis.

Abstract

To study the effects of brucine on vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) in a nude mouse model of bone metastasis due to breast cancer

and to assess the possible antitumor mechanism of brucine. A syringe needle was used to directly inject 0.2 mL monoplast suspension (with 2×105 human breast cancer cells contained) into the bony femoral cortex of the right hind leg for modeling. Twenty-five nude mice were randomized into five groups and administered with an intraperitoneal injection of saline or drug for 8 consecutive days: model group (0.2 mL normal saline)

low-dose brucine group (1.73 mg·kg−1)

medium-dose brucine group (3.45 mg·kg−1)

high-dose brucine group (6.90 mg·kg−1)

and thalidomide group (200 mg·kg−1). Diet and activity were recorded

and the tumors were harvested 5 weeks later. The percentage of VEGF-positive cells was determined with hematoxylin and eosin staining and immunohistochemical staining

and MVD expression was determined by optical microscopy. The VEGF expressions in brucine- or thalidomide-treated mice were significantly reduced as compared with mice in the model group (P <0.01). There were no significant difference between the high-dose brucine group and the thalidomide group (P >0.05). Significant difference was between the high- and low-dose brucine group P<0.05). Further

VEGF expression was significantly increased in the low- and medium-dose brucine groups compared with the thalidomide group (P <0.05). The MVD values in the three brucine and thalidomide groups were significantly lower than that in the model group (P <0.01). The MVD values in the medium- and high-dose brucine groups were not significantly different from those in the thalidomide group (P >0.05)

while the MVD value showed a significant increase in the low-dose group compared with the thalidomide group (P <0.05). Brucine could inhibit the growth of breast cancer to bone metastases

possibly by inhibiting tumor angiogenesis.

关键词

BrucineBreast CancerBone Metastasisvascular endothelial growth factor expressionmicrovessel density

Keywords

BrucineBreast CancerBone Metastasisvascular endothelial growth factor expressionmicrovessel density

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Department of Scientific Research and Education, Beijing University of Chinese Medicine Third Affiliated Hospital

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Beijing University of Chinese Medicine

Department of Chinese Medicine Tumor, the Affiliated Provincial Hospital of Anhui Medical University

Department of Geriatrics, the First Affiliated Hospital of Bengbu Medical College

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