Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms

Zhi-ming Gao; Li Yang; Feng Huang; Ai-hua Xiong; Ning Zhou; Lan Zhou; Kong-yan Li; Jie-li Deng; Kai-yu Li; Wei Liu; Yao Chen; Wan-ting Luo; Hong Nie

doi:10.1007/s11655-012-1190-z

Your Location：

Home >

Browse articles >

Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms

Updated：2021-08-27

- Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms
- Chinese Journal of Integrative Medicine Vol. 19, Issue 11, Pages: 844-852(2013)
- Affiliations：
  
  1. Department of Phamacy, Zhuhai Municipal People’s Hospital,Guangdong Province,Zhuhai,China
  2. College of Pharmacy, Jinan University,Guangzhou,China
  3. Guizhou Tongjitang Pharmaceutical Co., Ltd.,Guiyang,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30400596), the 211 Project of Jinan University, the Fundamental Research Funds for the Central Universities, Grants from Natural Science Foundation of Jinan University (No. 51204017) and Research Projects of Jinan University Challenge Cup Extracurricular Scientific Activities
- DOI：10.1007/s11655-012-1190-z
  CLC：
- Published：2013，
  
  Published Online：22 October 2012，
Scan for full text
Gao, Zm., Yang, L., Huang, F. et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms., Chin. J. Integr. Med. 19, 844–852 (2013). https://doi.org/10.1007/s11655-012-1190-z

Zhi-ming Gao, Li Yang, Feng Huang, et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms. [J]. Chinese Journal of Integrative Medicine 19(11):844-852(2013)
Gao, Zm., Yang, L., Huang, F. et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms., Chin. J. Integr. Med. 19, 844–852 (2013). https://doi.org/10.1007/s11655-012-1190-z DOI：

Zhi-ming Gao, Li Yang, Feng Huang, et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms. [J]. Chinese Journal of Integrative Medicine 19(11):844-852(2013) DOI： 10.1007/s11655-012-1190-z.

摘要

To investigate the preventive effects and possible underlying mechanism of different extracts of Kanggushu (抗骨疏) on osteoporosis in ovariectomized rats. One hundred and sixtyfive female SD rats were divided into 11 groups: control

sham

model

Xianling Gubao Capsule (仙灵骨葆胶囊)

nilestriol

Kanggushu aqueous extract high-

medium-

and low-dose and suet extract high-

medium-

and low-dose groups. The osteoporosis model was made by ovariectomizing the rats. The latter 8 groups were administered intragastricly with Xianling Gubao Capsule

nilestriol

Kanggushu aqueous extract and suet extract for 12 weeks

respectively

while the other 3 groups were administered orally saline. The whole body bone mineral density

bone mineral content

organ coefficient of uterus

serum estradiol and alkaline phosphatase contents

blood calcium

phosphorus

interleukin 6 and bone Gla-protein levels after treatment were monitored. Additionally

three-point bending test of femur

HE staining

and scanning electron microscope were performed to explore the pharmacodynamics and underlying mechanisms. In comparison with ovariectomized rats of model group

Kanggushu aqueous extract high-dose resulted in an increased bone mineral density

bone mineral content and organ coefficient of uterus

improved estradiol level

and improved maximum load and structural stiffness (P<0.05 or P<0.01). Two-dimensional and three-dimensional trabecular structure was also observed under HE staining and scanning electron microscopy

and the number and thickness of trabecular bone in Kanggushu aqueous extract high-dose group was significantly increased compared to the model group

while the lipid droplets in bone marrow cavity were significantly less. However

there were no significant differences in blood calcium

total serum alkaline phosphatase and bone Gla protein among different treatment groups. Overall

the osteoprotective effects of Kanggushu aqueous extract were comparable to those of nilestriol and were significantly more effective than those of Xianling Gubao Capsule. The preventive effects of Kanggushu aqueous extract might be partly due to the increased estradiol level

accelerated restoration of bone trabecular reticulate structure

and accordingly increased bone mineral density in osteoporosis rats.

Abstract

sham

model

Xianling Gubao Capsule (仙灵骨葆胶囊)

nilestriol

Kanggushu aqueous extract high-

medium-

and low-dose and suet extract high-

medium-

and low-dose groups. The osteoporosis model was made by ovariectomizing the rats. The latter 8 groups were administered intragastricly with Xianling Gubao Capsule

nilestriol

Kanggushu aqueous extract and suet extract for 12 weeks

respectively

while the other 3 groups were administered orally saline. The whole body bone mineral density

bone mineral content

organ coefficient of uterus

serum estradiol and alkaline phosphatase contents

blood calcium

phosphorus

interleukin 6 and bone Gla-protein levels after treatment were monitored. Additionally

three-point bending test of femur

HE staining

and scanning electron microscope were performed to explore the pharmacodynamics and underlying mechanisms. In comparison with ovariectomized rats of model group

Kanggushu aqueous extract high-dose resulted in an increased bone mineral density

bone mineral content and organ coefficient of uterus

improved estradiol level

and improved maximum load and structural stiffness (P<0.05 or P<0.01). Two-dimensional and three-dimensional trabecular structure was also observed under HE staining and scanning electron microscopy

and the number and thickness of trabecular bone in Kanggushu aqueous extract high-dose group was significantly increased compared to the model group

while the lipid droplets in bone marrow cavity were significantly less. However

there were no significant differences in blood calcium

total serum alkaline phosphatase and bone Gla protein among different treatment groups. Overall

accelerated restoration of bone trabecular reticulate structure

and accordingly increased bone mineral density in osteoporosis rats.

关键词

Kanggushu extractXianling Gubao CapsuleOsteoporosisestrogenmechanism

Keywords

Kanggushu extractXianling Gubao CapsuleOsteoporosisestrogenmechanism

references

Riggs BL, Melton LJ 3rd. Involutional osteoporosis. N Engl J Med 1986;314:1676–1686.

Manolagas SC. Birth and death of bone cells: basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev 2000;21:115–137.

Lindsay R. Sex steroids in the pathogenesis and prevention of osteoporosis. In: Riggs BL, ed. Osteoporosis: etiology, diagnosis and management. New York: Raven Press;1988:333–358.

Lewiecki EM. Management of osteoporosis. Clin Mol Allergy 2004;2:9.

Riggs BL, Melton LJ 3rd. The worldwide problem of osteoporosis: insights afforded by epidemiology. Bone 1995;17:505–511.

Cooper C, Cole ZA, Holroyd CR, Earl SC, Harvey NC, Dennison EM, et al. Secular trends in the incidence of hip and other osteoporotic fractures. Osteoporos Int 2011;22:1277–1288.

Cooper C, Campion G, Melton LJ 3rd. Hip fractures in the elderly: a world-wide projection. Osteoporos Int 1992;2:285–289.

Zhao WY, Dong BR, Ou XM, Zhang YF, Zhou Y. New development and evidence of the treatment of osteoporosis drugs. Chin J Osteoporosis (Chin) 2003;9:80–82.

Zhao YF, Shi YY, Shen PZ, Xu Y, Wang HB. Effects of Xianling Gubao on the bone histomorphometry in ovariectomized rats. J Tradit Chin Orthoped Traumatol (Chin) 2004;12:3–5.

Jiang YL, Huo HR, Li LF, Guo SY. Effects of 15 kinds of traditional Chinese and Western medicines on proliferation and maturation of osteoblasts in rats. Chin J Osteoporosis (Chin) 2008;8:342–345.

Ren GY, Lu L, Zhou N, Zhang GL, Wang JP. Effects of Xianling Gubao Capsule on the growth hormone concentration and transforming growth factor-β expression during fracture healing progress. Guizhou Med (Chin) 2003;27:595–597.

Cui SZ, Hu YL. Effects of Xianling Gubao Capsule on biochemical indicators of bone metabolism in ovariectomized rats. Chin J Clin Med Pract (Chin) 2007;16:260–262.

Zhang Y, Wu CF. Development of Chinese agents Xianling Gubao on the treatment of osteoporosis. Inf Tradit Chin Med (Chin) 2007;24:16–17.

Zhang XZ, Han JF, Qian GF, He M, Li Y, Gu L. Effects of Xianling Gubao on the bone mineral density, IL-6, TNF-a, and IGF-I in PMO. Chin J Osteoporosis (Chin) 2004;10:90–93.

Kalu DN. The ovariectomized rat model of postmenopausal bone loss. Bone Miner 1991;15:175–191.

Xiao JD, ed. Practical osteoporosis study. 1st ed. Beijing: Science Press;2004:152.

Cao WH, Yu LJ. Postmenopausal patients with estrogen. China Med Abstracts Geriatr (Chin) 2000;3:196–198.

Wang Y, Liu CL, Yu XW. Effects of estrogen and estrogen receptor in adipose tissue. Int Med J (Maternal and Child Health, Chin) 2003;14:94–95.

Zhang GA, Liu XM, Shi SL, Chen ZR, Shi YB. The relationship between blood IL-6 and E2 level and compact bone substance density in perimenopausal and postmenopausal women. Shanghai J Immunol (Chin)1999;19:35–37.

FULL TEXT LINK

More>

Views

575

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Compound Recipe Gengniankang on senile sexual hormone and expression of estrogen receptor in bone of climacteric female rats

Buyang Huanwu Decoction (补阳还五汤) reduces infarct volume and enhances estradiol and estradiol receptor concentration in ovariectomized rats after middle cerebral artery occlusion

Effect of oviductus ranae and oviductus ranae eggs on bone metabolism and osteoporosis

Study of osteoporosis treatment principles used historically by ancient physicians in Chinese Medicine

Antifibrotic effect of the Chinese herbs Modified Danggui Buxue Decoction (加味当归补血汤) on adriamycin-induced nephropathy in rats

Related Author

No data

Related Institution

Department of Obstetrics and Gynecology, The First Hospital, Shanxi Medical University

Key Laboratory of Internal Medicine, Hunan University of Traditional Chinese Medicine

Institute of Military Veterinary, Academy of Military Medical Sciences

Institute of Radiation Medicine, Fudan University

Beijing Tongrentang Biological Product Development Co., Ltd.

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰