Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction

Chung-hsin Yeh; Yu-yen Yang; Ya-fang Huang; Kuan-chih Chow; Ming-feng Chen

doi:10.1007/s11655-012-1206-8

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Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction

Updated：2021-08-27

- Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction
- Chinese Journal of Integrative Medicine Vol. 18, Issue 9, Pages: 676-682(2012)
- Affiliations：
  
  1. Department of Neurology, Show Chwan Memorial Hospital, Changhua,Taiwan,China
  2. Graduate Institute of Life Sciences, National Chung Hsing University, Taichung,Taiwan,China
  3. Department of Medical Research, Show Chwan Memorial Hospital, Changhua,Taiwan,China
  4. Graduate Institute of Biomedical Sciences, National Chung Hsing University, Taichung,Taiwan,China
  5. Department of Gastroenterology and Heaptology, E-DA Hospital, Kaohsiung,Taiwan,China
  6. Graduate Institute of Integrated Medicine, China Medical University, Taichung,Taiwan,China
- Author bio：
- Funds：
  
  Supported by Show Chwan Memorial Hospital
- DOI：10.1007/s11655-012-1206-8
  CLC：
- Published：2012，
  
  Published Online：31 August 2012，
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Yeh, Ch., Yang, Yy., Huang, Yf. et al. Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction., Chin. J. Integr. Med. 18, 676–682 (2012). https://doi.org/10.1007/s11655-012-1206-8

Chung-hsin Yeh, Yu-yen Yang, Ya-fang Huang, et al. Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction. [J]. Chinese Journal of Integrative Medicine 18(9):676-682(2012)
Yeh, Ch., Yang, Yy., Huang, Yf. et al. Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction., Chin. J. Integr. Med. 18, 676–682 (2012). https://doi.org/10.1007/s11655-012-1206-8 DOI：

Chung-hsin Yeh, Yu-yen Yang, Ya-fang Huang, et al. Induction of apoptosis in human Hep3B hepatoma cells by norcantharidin through a p53 independent pathway via TRAIL/DR5 signal transduction. [J]. Chinese Journal of Integrative Medicine 18(9):676-682(2012) DOI： 10.1007/s11655-012-1206-8.

摘要

To investigate the inhibitory activities of norcantharidin (NCTD)

a demethylated analogue of cantharidin

on Hep3B cells (a human hepatoma cell line) with deficiency of p53. The survival rate of the Hep3B cells after treating with NCTD was measured by MTT assay. Cell cycle of treated cells was analyzed by flow cytometry

and DNA fragmentation was observed by electrophoresis. The influence of inhibitors for various caspases and anti-death receptors antibodies on the NCTD-induced apoptosis in the cells was determined. NCTD treatment resulted in growth inhibition of Hep3B cells in a dose- and time-dependent manner. Cell cycle analysis of the cells after treatment with NCTD for 48 h shows that NCTD induced G2M phase arrest occurs at low concentration (⩽ 25 μmol/L) but G0G1 phase arrest at high concentration (50 μmol/L). The addition of both caspase-3 and caspase-10 inhibitors completely inhibited DNA fragmentation. Addition of anti-TRAIL/DR5 antibody significantly inhibited DNA fragmentation. NCTD may inhibit the proliferation of Hep3B cells by arresting cell cycle at G2M or G0G1 phase

and induce cells apoptosis via TRAIL/DR5 signal transduction through activation of caspase-3 and caspase-10 by a p53-independent pathway.

Abstract

To investigate the inhibitory activities of norcantharidin (NCTD)

a demethylated analogue of cantharidin

and induce cells apoptosis via TRAIL/DR5 signal transduction through activation of caspase-3 and caspase-10 by a p53-independent pathway.

关键词

norcantharidincaspaseapoptosisdeath receptors

Keywords

norcantharidincaspaseapoptosisdeath receptors

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