Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells

Xiao-yan Dang; Lei Dong; Hai-tao Shi; Bai-cang Zou

doi:10.1007/s11655-012-1208-6

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Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells

Updated：2021-08-27

- Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells
- Chinese Journal of Integrative Medicine Vol. 19, Issue 2, Pages: 119-126(2013)
- Affiliations：
  
  1. Department of Gastroenterology, the Second Affiliated Hospital, Xi’an Jiaotong University,Xi’an,China
  2. Department of Emergency, the Second Affiliated Hospital, Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by “13115” Program of Shaanxi Province (No. 2007ZDKG-73)
- DOI：10.1007/s11655-012-1208-6
  CLC：
- Published：2013，
  
  Published Online：21 September 2012，
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Dang, Xy., Dong, L., Shi, Ht. et al. Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells., Chin. J. Integr. Med. 19, 119–126 (2013). https://doi.org/10.1007/s11655-012-1208-6

Xiao-yan Dang, Lei Dong, Hai-tao Shi, et al. Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells. [J]. Chinese Journal of Integrative Medicine 19(2):119-126(2013)
Dang, Xy., Dong, L., Shi, Ht. et al. Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells., Chin. J. Integr. Med. 19, 119–126 (2013). https://doi.org/10.1007/s11655-012-1208-6 DOI：

Xiao-yan Dang, Lei Dong, Hai-tao Shi, et al. Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells. [J]. Chinese Journal of Integrative Medicine 19(2):119-126(2013) DOI： 10.1007/s11655-012-1208-6.

摘要

To investigate the effects of serum containing Chinese medicine (CM) Sanpi Pingwei (散癖平胃

SPPW) formula on the proliferation and apoptosis of human SGC-7901 cells and the possible mechanism. Serum containing CM SPPW formula (SPPW serum) was prepared by a serum pharmacology method. Human SGC-7901 cells were incubated with SPPW serum at three different concentrations and with the anticancer drug 5-fluorouracil (5-FU)

respectively. Cell proliferation was assessed by MTT assay

and cell apoptosis was detected by flow cytometry assay. Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot assay were employed to confirm the expressions of Bcl-2

Bax and p53 in SGC-7901 cells at mRNA and protein levels

respectively. SPPW serum suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. The colony forming rate of negative control was 48.2%

while those in the three SPPW serum groups and the 5-FU group decreased significantly (P<0.01). The number of colony forming units in the SPPW high dosage group was significantly smaller than that in the 5-FU group (P<0.01). MTT assay showed that SPPW serum restrained the proliferation of SGC-7901 cells

and the inhibition rate increased significantly in a dose-dependent manner. Annexin V/PI Assay suggested that SPPW serum induced the apoptosis of SGC-7901 cells significantly. RT-PCR and western blot assay indicated that SPPW serum upregulated the protein and mRNA expression levels of Bax and p53 in SGC-7901 cells

but downregulated the protein and mRNA expressions of Bcl-2. SPPW formula inhibits the proliferation of SGC-7901 cells in vitro and induces the cell apoptosis. It plays an anticancer role by regulating the expressions of Bax

p53 and Bcl-2 in SGC-7901 cells.

Abstract

To investigate the effects of serum containing Chinese medicine (CM) Sanpi Pingwei (散癖平胃

respectively. Cell proliferation was assessed by MTT assay

and cell apoptosis was detected by flow cytometry assay. Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot assay were employed to confirm the expressions of Bcl-2

Bax and p53 in SGC-7901 cells at mRNA and protein levels

respectively. SPPW serum suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. The colony forming rate of negative control was 48.2%

p53 and Bcl-2 in SGC-7901 cells.

关键词

stomach neoplasmsChinese Medicineapoptosis

Keywords

stomach neoplasmsChinese Medicineapoptosis

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Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

Shanghai Key Laboratory of Complex Prescription

Chinese Medicine Hospital of Shanxi Province

Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine

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