Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice

Li-ming Yin; Hui-fang Jiang; Xiao Wang; Xu-dai Qian; Rui-lan Gao; Xiao-jie Lin; Xiao-hong Chen; Ling-cong Wang

doi:10.1007/s11655-012-1210-z

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Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice

Updated：2021-08-27

- Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice
- Chinese Journal of Integrative Medicine Vol. 19, Issue 5, Pages: 360-366(2013)
- Affiliations：
  
  1. Research Institute of Hematopathy, the First Affiliated Hospital, Zhejiang Chinese Medical University,Hangzhou,China
  2. Tongde Hospital of Zhejiang Province,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by Zhejiang Provincial Natural Science Foundation of China (No. Y207728 and No. Y2080036) and Traditional Chinese Medicine Administration Bureau of Zhejiang Province, China (No. 2008YA005)
- DOI：10.1007/s11655-012-1210-z
  CLC：
- Published：2013，
  
  Published Online：21 September 2012，
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Yin, Lm., Jiang, Hf., Wang, X. et al. Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice., Chin. J. Integr. Med. 19, 360–366 (2013). https://doi.org/10.1007/s11655-012-1210-z

Li-ming Yin, Hui-fang Jiang, Xiao Wang, et al. Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice. [J]. Chinese Journal of Integrative Medicine 19(5):360-366(2013)
Yin, Lm., Jiang, Hf., Wang, X. et al. Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice., Chin. J. Integr. Med. 19, 360–366 (2013). https://doi.org/10.1007/s11655-012-1210-z DOI：

Li-ming Yin, Hui-fang Jiang, Xiao Wang, et al. Effects of sodium copper chlorophyllin on mesenchymal stem cell function in aplastic anemia mice. [J]. Chinese Journal of Integrative Medicine 19(5):360-366(2013) DOI： 10.1007/s11655-012-1210-z.

摘要

To investigate the effects of sodium copper chlorophyllin (SCC) on the proliferation

differentiation and immunomodulatory function of mesenchymal stem cells (MSCs) from mice with aplastic anemia. A mouse model of aplastic anemia was established by exposure of BALB/c mice to sublethal doses of 5.0 Gy Co60 γ radiation

followed by transplantation of 2×106 lymph node cells from DBA/2 donor mice within 4 h after radiation. Aplastic anemic BALB/c mice were randomly divided into six groups: the treated groups

which received 25

or 100 mg/kg/day SCC

respectively; a positive control group treated with cyclosporine A (CsA); and an untreated model control group (model group); while

the non-irradiated mice as the normal control group. SCC or CsA were administered by gastrogavage for 20 days

starting on day 4 after irradiation. Peripheral blood cells were counted and colony-forming fibroblasts (CFU-F) in the bone marrow were assayed. The ability of MSCs to form calcium nodes after culture in osteoinductive medium was also observed. The immunosuppressive effect of MSCs on T lymphocytes was analyzed by enzyme-linked immunosorbent assay and flow cytometry

to evaluate the efficacy of SCC in mice with aplastic anemia. Peripheral blood white cell and platelet counts were increased by medium and high SCC doses

compared with the untreated control. CFU-Fs were also increased compared with the untreated control

and the numbers of calcium nodes in MSCs in osteoinductive medium were elevated in response to SCC treatment. The percentage of Forkhead box protein 3 (FOXP3+) T cells was increased in T cell-MSC cocultures

and the cytokine transforming growth factor β1 was up-regulated in SCC-treated groups. The results of this study suggest that SCC not only promotes the proliferation and differentiation of MSCs

but also improves their immunoregulatory capacity in mice with aplastic anemia.

Abstract

To investigate the effects of sodium copper chlorophyllin (SCC) on the proliferation

followed by transplantation of 2×106 lymph node cells from DBA/2 donor mice within 4 h after radiation. Aplastic anemic BALB/c mice were randomly divided into six groups: the treated groups

which received 25

or 100 mg/kg/day SCC

respectively; a positive control group treated with cyclosporine A (CsA); and an untreated model control group (model group); while

the non-irradiated mice as the normal control group. SCC or CsA were administered by gastrogavage for 20 days

to evaluate the efficacy of SCC in mice with aplastic anemia. Peripheral blood white cell and platelet counts were increased by medium and high SCC doses

compared with the untreated control. CFU-Fs were also increased compared with the untreated control

and the cytokine transforming growth factor β1 was up-regulated in SCC-treated groups. The results of this study suggest that SCC not only promotes the proliferation and differentiation of MSCs

but also improves their immunoregulatory capacity in mice with aplastic anemia.

关键词

sodium copper chlorophyllinmesenchymal stem cellproliferationdifferentiationimmune modulation

Keywords

sodium copper chlorophyllinmesenchymal stem cellproliferationdifferentiationimmune modulation

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The Third People’s Hospital of Hangzhou

Department of Oncology, Zhejiang Provincial People’s Hospital

Institution of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University

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Shanghai Key Laboratory of Complex Prescription

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