Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics

Yong Wang; Wen-jing Chuo; Chun Li; Shu-zhen Guo; Jian-xin Chen; Jun-da Yu; Wei Wang

doi:10.1007/s11655-012-1230-8

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Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics

Updated：2021-08-27

- Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics
- Chinese Journal of Integrative Medicine Vol. 19, Issue 8, Pages: 616-620(2013)
- Affiliations：
  
  Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Creation for Significant New Drugs (2012ZX09103-201-011), National Science and Technology Pillar Program (No. 2012BAI29B07), and Project of Beijing University of Chinese Medicine (No. 2011-JYBZZ-JS055)
- DOI：10.1007/s11655-012-1230-8
  CLC：
- Published：2013，
  
  Published Online：3 December 2012，
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Wang, Y., Chuo, Wj., Li, C. et al. Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics., Chin. J. Integr. Med. 19, 616–620 (2013). https://doi.org/10.1007/s11655-012-1230-8

Yong Wang, Wen-jing Chuo, Chun Li, et al. Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics. [J]. Chinese Journal of Integrative Medicine 19(8):616-620(2013)
Wang, Y., Chuo, Wj., Li, C. et al. Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics., Chin. J. Integr. Med. 19, 616–620 (2013). https://doi.org/10.1007/s11655-012-1230-8 DOI：

Yong Wang, Wen-jing Chuo, Chun Li, et al. Energy metabolism disorder and myocardial injury in chronic myocardial ischemia with Qi deficiency and blood stasis syndrome based on 2-DE proteomics. [J]. Chinese Journal of Integrative Medicine 19(8):616-620(2013) DOI： 10.1007/s11655-012-1230-8.

摘要

To inquire the characteristic proteins in chronic myocardial ischemia by testing twodimensional electrophoresis (2-DE) map to explore the possible inherent pathological mechanism and the therapeutic intervention of qi deficiency and blood stasis syndrome. Ameroid constrictor ring was placed on the first interval of left anterior descending coronary artery to prepare chronic myocardial ischemia model on Chinese miniature swine. Animals were randomly divided into sham group and model group with 10 animals in each group

respectively. The dynamic symptoms observation of the four diagnostic information was collected from 0 to 12 weeks. Echocardiography was employed to evaluate cardiac function and the degree of myocardial ischemia

2-DE and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) were used to carry out proteomics research on animals. Enzyme-linked immunosorbent assay was applied to identify the relevant differential proteins on chronic myocardial ischemia with qi deficiency and blood stasis syndrome. The preliminary study found that at the 12th week

chronic myocardial ischemia with qi deficiency and blood stasis syndrome model was established stably. Compared with the sham group

there were 8 different proteins down-regulated

22 proteins up-regulated significantly. After validated by MALDITOF-MS/MS

11 protein spots were identified. Distinct proteins were mainly associated with energy metabolism and myocardial structural injury

including isocitrate dehydrogenase 3 (NAD+) alpha

NADH dehydrogenase (NAD) Fe-S protein 1

chain A (crystal structure of aldose reductase by binding domain reveals a new Nadph)

heat shock protein 27 (HSP27)

oxidoreductase (NAD-binding protein)

antioxidant protein isoform

cardiac troponin T (cTnT)

myosin (myosin light polypeptide)

cardiac alpha tropomyosin

apolipoprotein A-I and albumin. Down-regulated energy metabolism disorder mediated by NADH respiratory chain and myocardial injury may be the pathogenesis of myocardial ischemia with qi deficiency and blood stasis syndrome. These proteins may be the potential diagnostic marker(s) for qi deficiency and blood stasis syndrome

finally provided new clues for new therapeutic drug target of Chinese medicine

Abstract

chronic myocardial ischemia with qi deficiency and blood stasis syndrome model was established stably. Compared with the sham group

there were 8 different proteins down-regulated

22 proteins up-regulated significantly. After validated by MALDITOF-MS/MS

11 protein spots were identified. Distinct proteins were mainly associated with energy metabolism and myocardial structural injury

including isocitrate dehydrogenase 3 (NAD+) alpha

NADH dehydrogenase (NAD) Fe-S protein 1

chain A (crystal structure of aldose reductase by binding domain reveals a new Nadph)

heat shock protein 27 (HSP27)

oxidoreductase (NAD-binding protein)

antioxidant protein isoform

cardiac troponin T (cTnT)

myosin (myosin light polypeptide)

cardiac alpha tropomyosin

finally provided new clues for new therapeutic drug target of Chinese medicine

关键词

chronic myocardial ischemiaChinese Medicineqi deficiency and blood stasis syndromeproteomics

Keywords

chronic myocardial ischemiaChinese Medicineqi deficiency and blood stasis syndromeproteomics

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