Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure

Fang-ning Wei; Zi-lin Chen; Hai-feng Yang; Ling Han; Hai-ming Ding; Shi-gui Deng; Run-mei Ou; Ai-hua Ou; Yan-fen Liang; Zi-heng Hu; Jian Wang; Xiao-hong Yang; Ni-zhi Yang

doi:10.1007/s11655-012-1233-5

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Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure

Updated：2021-08-27

- Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure
- Chinese Journal of Integrative Medicine Vol. 19, Issue 8, Pages: 589-595(2013)
- Affiliations：
  
  1. Department of Urology, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China
  2. Xinhui Hospital of Traditional Chinese Medicine, Jiangmen, Guandong Province,China
  3. Department of Pathology, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China
  4. Guangdong Provincial Scientific Institute of Traditional Chinese Medicine,Guangzhou,China
  5. Clinical Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China
  6. Animal Laboratory Center, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China
  7. Epidemiological Teaching and Research Section, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China
  8. Pharmaceutical Manufacturing Section, Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,China
  9. Guangzhou University of Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the Plan Program of Guangdong Science and Technology Department (No. 2006B35601010)
- DOI：10.1007/s11655-012-1233-5
  CLC：
- Published：2013，
  
  Published Online：3 December 2012，
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Wei, Fn., Chen, Zl., Yang, Hf. et al. Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure., Chin. J. Integr. Med. 19, 589–595 (2013). https://doi.org/10.1007/s11655-012-1233-5

Fang-ning Wei, Zi-lin Chen, Hai-feng Yang, et al. Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure. [J]. Chinese Journal of Integrative Medicine 19(8):589-595(2013)
Wei, Fn., Chen, Zl., Yang, Hf. et al. Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure., Chin. J. Integr. Med. 19, 589–595 (2013). https://doi.org/10.1007/s11655-012-1233-5 DOI：

Fang-ning Wei, Zi-lin Chen, Hai-feng Yang, et al. Effect of Sanqi Oral Liquid (三芪口服液) on the expressions of CD4+, CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure. [J]. Chinese Journal of Integrative Medicine 19(8):589-595(2013) DOI： 10.1007/s11655-012-1233-5.

摘要

To explore the mechanisms of Chinese herbal medicine Sanqi Oral Liquid (三芪口服液)

composed of Astragalus membranaceus and Panpax notoginseng

in alleviating renal injury by observing its effect on the expressions of CD4+

CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure. A total of 102 SD rats were randomly divided into six groups: three treatment groups were administrated with high

medium and low dosage of Sanqi Oral Liquid respectively by gavage; a normal group

a 5/6 nephrectomized model group

and a group treated with coated aldehyde oxygenstarch were used as controls. Following oral administration of Sanqi Oral Liquid for 12 weeks

the general condition and renal pathological changes were observed

and the renal function

platelet count (PLT) and the expressions of CD4+

CD8+ and CD68+ cells were determined for each group. There were proliferation of mesangial matrix

renaltubularnecrosis and obvious tubulointerstitial fibrosis in the model group

and they were much milder in the treatment groups. Compared with the model group

the amounts of blood urea nitrogen (BUN)

serum creatinine (Scr) and PLT in the treatment groups decreased (P<0.05 for all); and in the group administrated of medium dosage of Sanqi Oral Liquid

the expression of CD4+ cells was up-regulated and those of CD8+ and CD68+ cells were down-regulated (P<0.05 for all)

leading to an increased ratio of CD4+/CD8+(P<0.01). Sanqi Oral Liquid has a significant effect on regulating lymphocyte subsets

reducing the infiltration of macrophages in renal tissues and alleviating tubulointerstitial fibrosis

and this may be one of mechanisms of Sanqi Oral Liquid in delaying the progression of chronic kidney diseases.

Abstract

To explore the mechanisms of Chinese herbal medicine Sanqi Oral Liquid (三芪口服液)

composed of Astragalus membranaceus and Panpax notoginseng

in alleviating renal injury by observing its effect on the expressions of CD4+

CD8+ and CD68+ cells in 5/6 nephrectomized rats with chronic renal failure. A total of 102 SD rats were randomly divided into six groups: three treatment groups were administrated with high

medium and low dosage of Sanqi Oral Liquid respectively by gavage; a normal group

a 5/6 nephrectomized model group

and a group treated with coated aldehyde oxygenstarch were used as controls. Following oral administration of Sanqi Oral Liquid for 12 weeks

the general condition and renal pathological changes were observed

and the renal function

platelet count (PLT) and the expressions of CD4+

CD8+ and CD68+ cells were determined for each group. There were proliferation of mesangial matrix

renaltubularnecrosis and obvious tubulointerstitial fibrosis in the model group

and they were much milder in the treatment groups. Compared with the model group

the amounts of blood urea nitrogen (BUN)

serum creatinine (Scr) and PLT in the treatment groups decreased (P<0.05 for all); and in the group administrated of medium dosage of Sanqi Oral Liquid

the expression of CD4+ cells was up-regulated and those of CD8+ and CD68+ cells were down-regulated (P<0.05 for all)

leading to an increased ratio of CD4+/CD8+(P<0.01). Sanqi Oral Liquid has a significant effect on regulating lymphocyte subsets

reducing the infiltration of macrophages in renal tissues and alleviating tubulointerstitial fibrosis

and this may be one of mechanisms of Sanqi Oral Liquid in delaying the progression of chronic kidney diseases.

关键词

Sanqi Oral Liquidchronic kidney diseasetubulointerstitial fibrosisAstragalus membranaceusPanpax notoginseng

Keywords

Sanqi Oral Liquidchronic kidney diseasetubulointerstitial fibrosisAstragalus membranaceusPanpax notoginseng

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Department of Nephrology, Guangdong Provincial Hospital of Chinese Medicine

Department of Nephrology, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine

Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV)00088, Chinese PLA General Hospital

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Traditional Chinese and Western Medicine, Peking University School of Oncology, Beijing Cancer Hospital and Institute

Department of Laboratory Medicine, Shenzhen Hospital Affiliated to Peking University

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