Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs

Jian-hua Fu; Yong-qiu Zheng; Peng Li; Xin-zhi Li; Xiao-hong Shang; Jian-xun Liu

doi:10.1007/s11655-012-1250-4

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Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs

Updated：2021-08-27

- Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs
- Chinese Journal of Integrative Medicine Vol. 19, Issue 8, Pages: 582-588(2013)
- Affiliations：
  
  Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by Project of National Natural Science Foundation of Beijing (No. 7092089) and the National Key New Drug Project (2009ZX09301-005)
- DOI：10.1007/s11655-012-1250-4
  CLC：
- Published：2013，
  
  Published Online：3 December 2012，
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Fu, Jh., Zheng, Yq., Li, P. et al. Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs., Chin. J. Integr. Med. 19, 582–588 (2013). https://doi.org/10.1007/s11655-012-1250-4

Jian-hua Fu, Yong-qiu Zheng, Peng Li, et al. Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs. [J]. Chinese Journal of Integrative Medicine 19(8):582-588(2013)
Fu, Jh., Zheng, Yq., Li, P. et al. Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs., Chin. J. Integr. Med. 19, 582–588 (2013). https://doi.org/10.1007/s11655-012-1250-4 DOI：

Jian-hua Fu, Yong-qiu Zheng, Peng Li, et al. Hawthorn leaves flavonoids decreases inflammation related to acute myocardial ischemia/reperfusion in anesthetized dogs. [J]. Chinese Journal of Integrative Medicine 19(8):582-588(2013) DOI： 10.1007/s11655-012-1250-4.

摘要

To investigate the effects and mechanisms of hawthorn leaves flavonoids (HLF) on acute myocardial ischemia/reperfusion in anesthetized dogs. The acute ischemia models were prepared by ligating left anterior descending (LAD) artery for 60 min. Qualified 15 male dogs were randomly divided into 3 groups with 5 in each group: blank control (treated with normal saline 3 mL/kg) group

HLF low dosage (5 mg/kg) group and high dosage (10 mg/kg) group

with an once injection through a femoral vein 5 min before reperfusion. Epicardial electrocardiogram was adopted to measure the scope and degree of myocardial ischemia. Simultaneously

neutrophil infiltration in infarct (Inf) and remote site (RS) of myocardial tissue was measured by myeloperoxidase (MPO) activity assay. The serum interleukin-1 (IL-1) and tumor necrosis factorα (TNF-α) content were quantified by radioimmuno-assay. Furthermore

expression of G protein-coupled receptor kinase 2 (GRK2) and nuclear factor κB (NF-κB) in Inf and RS tissue were detected by Western blotting technique. Ischemia and reperfusion increased the MPO activity and IL-1 and TNF-α content. HLF (10 and 5 mg/kg) could significantly decrease the degree and scope of myocardial ischemia; markedly inhibit the increase of MPO activity

and IL-1 and TNF-α content induced by myocardial ischemia/infarction. Furthermore

HLF increased GRK2 expression and inhibited NF-κB expression in Inf tissue. HLF could improve the situation of acute myocardial ischemia and inhibit the inflammation in anesthetized dogs

which might be due to its increasing effect on the GRK2 and NF-κB expressions.

Abstract

HLF low dosage (5 mg/kg) group and high dosage (10 mg/kg) group

with an once injection through a femoral vein 5 min before reperfusion. Epicardial electrocardiogram was adopted to measure the scope and degree of myocardial ischemia. Simultaneously

and IL-1 and TNF-α content induced by myocardial ischemia/infarction. Furthermore

HLF increased GRK2 expression and inhibited NF-κB expression in Inf tissue. HLF could improve the situation of acute myocardial ischemia and inhibit the inflammation in anesthetized dogs

which might be due to its increasing effect on the GRK2 and NF-κB expressions.

关键词

hawthorn leaves flavonoidsacute myocardial ischemiareperfusioninflammationG protein-coupled receptor kinase 2

Keywords

hawthorn leaves flavonoidsacute myocardial ischemiareperfusioninflammationG protein-coupled receptor kinase 2

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Department of Integrative Cardiology, China-Japan Friendship Hospital

Department of Cardiology, China-Japan Friendship Hospital

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Ministry of Education), Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College

Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University

Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine

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