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Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B
- Chinese Journal of Integrative Medicine Vol. 19, Issue 10, Pages: 741-748(2013)
- Affiliations：
1. Department of Dermatology, The Chinese People’s Liberation Army General Hospital,Beijing,China
2. Department of Dermatology, The Navy Headquarters Clinic,Beijing,China
3. Department of Dermatology, First Affiliated Hospital of Chinese People’s Liberation Army General Hospital,Beijing,China
- Author bio：
- Funds：
DOI：10.1007/s11655-012-1251-3
CLC：Published：2013，
Published Online：3 December 2012，
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Jiao, Zl., Li, L., Zhao, Zg. et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B., Chin. J. Integr. Med. 19, 741–748 (2013). https://doi.org/10.1007/s11655-012-1251-3
Ze-long Jiao, Lei Li, Zi-gang Zhao, et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B. [J]. Chinese Journal of Integrative Medicine 19(10):741-748(2013)
Jiao, Zl., Li, L., Zhao, Zg. et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B., Chin. J. Integr. Med. 19, 741–748 (2013). https://doi.org/10.1007/s11655-012-1251-3 DOI：
Ze-long Jiao, Lei Li, Zi-gang Zhao, et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B. [J]. Chinese Journal of Integrative Medicine 19(10):741-748(2013) DOI： 10.1007/s11655-012-1251-3.
摘要
To investigate the antiinflammatory activities of aqueous extract of Occimum gratissmium (OGE) with emphasis on expression of proinflammatory cytokines in Lipopolysaccharide (LPS)-stimulated epithelial cell BEAS-2B. Effects of OGE on cell viability were determined by MTT assay. mRNA expression were analyzed by and reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Activation of kinase cascades was investigated by immunoblot. Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry. OGE (<200 μg/mL) treatment or pretreatment and following LPS exposure slightly affected viability of BEAS-2B cells. Increase of interleukin (IL)-6 and IL-8 and the elevated level of intracellular ROS in LPS-stimulated BEAS-2B cells were diminished by OGE pretreatment in a dose-dependent manner. OGE suppressed inflammatory response-associated mitogen-activated protein kinases (MAPKs) and Akt activation. Additionally
OGE pretreatment increased level of cellular inhibitor of κBα (IκBα) and inhibited nuclear translocation of nuclear factor kappa B (NF-κB). These findings indicate that significant suppression of IL-6 and IL-8 expressions in LPS-stimulated BEAS-2B cells by OGE may be attributed to inhibiting activation of MAPKs and Akt and consequently suppressing nuclear translocation of NF-κB.
Abstract
To investigate the antiinflammatory activities of aqueous extract of Occimum gratissmium (OGE) with emphasis on expression of proinflammatory cytokines in Lipopolysaccharide (LPS)-stimulated epithelial cell BEAS-2B. Effects of OGE on cell viability were determined by MTT assay. mRNA expression were analyzed by and reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Activation of kinase cascades was investigated by immunoblot. Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry. OGE (<200 μg/mL) treatment or pretreatment and following LPS exposure slightly affected viability of BEAS-2B cells. Increase of interleukin (IL)-6 and IL-8 and the elevated level of intracellular ROS in LPS-stimulated BEAS-2B cells were diminished by OGE pretreatment in a dose-dependent manner. OGE suppressed inflammatory response-associated mitogen-activated protein kinases (MAPKs) and Akt activation. Additionally
OGE pretreatment increased level of cellular inhibitor of κBα (IκBα) and inhibited nuclear translocation of nuclear factor kappa B (NF-κB). These findings indicate that significant suppression of IL-6 and IL-8 expressions in LPS-stimulated BEAS-2B cells by OGE may be attributed to inhibiting activation of MAPKs and Akt and consequently suppressing nuclear translocation of NF-κB.
关键词
Ocimum grasstimumantiinflammationlipopolysaccharidesepithelial cell
Keywords
Ocimum grasstimumantiinflammationlipopolysaccharidesepithelial cell
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