Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis

Zhen-feng Hong; Jiu-mao Lin; Xiao-yong Zhong; Ying Li; Jian-heng Zhou; Wei Xu; Jun Peng

doi:10.1007/s11655-012-1264-y

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Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis

Updated：2021-08-27

- Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis
- Chinese Journal of Integrative Medicine Vol. 18, Issue 11, Pages: 824-830(2012)
- Affiliations：
  
  1. Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  4. Department of Pharmacology, Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072927 and No. 81173433), the Natural Science Foundation of Fujian Province of China (No. 2010J01199 and No. 2009J01169)
- DOI：10.1007/s11655-012-1264-y
  CLC：
- Published：2012，
  
  Published Online：20 October 2012，
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Hong, Zf., Lin, Jm., Zhong, Xy. et al. Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis., Chin. J. Integr. Med. 18, 824–830 (2012). https://doi.org/10.1007/s11655-012-1264-y

Zhen-feng Hong, Jiu-mao Lin, Xiao-yong Zhong, et al. Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis. [J]. Chinese Journal of Integrative Medicine 18(11):824-830(2012)
Hong, Zf., Lin, Jm., Zhong, Xy. et al. Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis., Chin. J. Integr. Med. 18, 824–830 (2012). https://doi.org/10.1007/s11655-012-1264-y DOI：

Zhen-feng Hong, Jiu-mao Lin, Xiao-yong Zhong, et al. Qianliening capsule (前列宁胶囊) inhibits human prostate cell growth via induction of mitochondrion-dependent cell apoptosis. [J]. Chinese Journal of Integrative Medicine 18(11):824-830(2012) DOI： 10.1007/s11655-012-1264-y.

摘要

To investigate the molecular mechanisms by which Qianliening Capsule (前列宁胶囊

QC) treats benign prostatic hyperplasia (BPH). Human prostate stromal cell line WPMY-1 was treated with 0

3 and 5 mg/mL of QC for 24

48 and 72 h

respectively

in the presence of 10 ng/mL basic fibroblast growth factor (bFGF). The viability of WPMY-1 cells was determined by 3-(4

5-Dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Cell morphology was observed by phase-contrast microscopy. 4′

6-diamidino-2-phenylindole (DAPI) staining and fluorescence activated cell sorting (FACS) analysis with Annexin-V/propidium iodide (PI) staining were performed to determine cell apoptosis. The loss of mitochondrial membrane potential was examined by FACS analysis with 5

5′

6′-tetrachloro-1

1′

3′-tetraethylbenzimidazolyl-carbocyarine iodide (JC-1) staining. Activation of caspase-3 and -9 was evaluated by colorimetric assay. The mRNA and protein expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting

respectively. Upon bFGF stimulation

the viability of WPMY-1 cells was increased to 122%–118% compared with the control cells (P <0.05). However

treatment with 1–5 mg/mL of QC for 24

48 and 72 h decreased the viability of bFGF-stimulated cells to 80%–92%

59%–82%

36%–62% compared with the untreated cells (P <0.05). In addition

QC treatment reduced WPMY-1 cell density in a dose-dependent manner. Moreover

QC treatment dose-dependently induced the loss of plasma membrane asymmetry

the nuclear condensation and fragmentation

collapse of mitochondrial membrane potential

activation of caspase-9 and caspase-3

and increase of pro-apoptotic Bax/Bcl-2 ratio. Promoting mitochondrion-dependent apoptosis of prostate stromal cells might be one of the mechanisms by which QC treats BPH.

Abstract

To investigate the molecular mechanisms by which Qianliening Capsule (前列宁胶囊

QC) treats benign prostatic hyperplasia (BPH). Human prostate stromal cell line WPMY-1 was treated with 0

3 and 5 mg/mL of QC for 24

48 and 72 h

respectively

in the presence of 10 ng/mL basic fibroblast growth factor (bFGF). The viability of WPMY-1 cells was determined by 3-(4

5-Dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay. Cell morphology was observed by phase-contrast microscopy. 4′

5′

6′-tetrachloro-1

1′

respectively. Upon bFGF stimulation

the viability of WPMY-1 cells was increased to 122%–118% compared with the control cells (P <0.05). However

treatment with 1–5 mg/mL of QC for 24

48 and 72 h decreased the viability of bFGF-stimulated cells to 80%–92%

59%–82%

36%–62% compared with the untreated cells (P <0.05). In addition

QC treatment reduced WPMY-1 cell density in a dose-dependent manner. Moreover

QC treatment dose-dependently induced the loss of plasma membrane asymmetry

the nuclear condensation and fragmentation

collapse of mitochondrial membrane potential

activation of caspase-9 and caspase-3

and increase of pro-apoptotic Bax/Bcl-2 ratio. Promoting mitochondrion-dependent apoptosis of prostate stromal cells might be one of the mechanisms by which QC treats BPH.

关键词

Qianliening CapsuleChinese Medicinebenign prostatic hyperplasiaapoptosisWPMY-1 cells

Keywords

Qianliening CapsuleChinese Medicinebenign prostatic hyperplasiaapoptosisWPMY-1 cells

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Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

Shanghai Key Laboratory of Complex Prescription

Chinese Medicine Hospital of Shanxi Province

Department of Traditional Chinese Medicine, Zhongshan Hospital, Xiamen University

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