Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation

Zhen-gao Sun; Fang Lian; Qing Jia; Jin-long Sun; Ting-ting Li; Ying Guo; Jian-wei Zhang; Ning Zhang; Hui Liu; Li-hong Wang

doi:10.1007/s11655-012-1273-7

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Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation

Updated：2021-08-27

- Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation
- Chinese Journal of Integrative Medicine Vol. 18, Issue 11, Pages: 846-849(2012)
- Affiliations：
  
  1. Reproductive Center, the Affiliated Hospital of Shandong, University of Traditional Chinese Medicine,Jinan,China
  2. Basic Medicine Research Institution, Shandong Academy of Medical Sciences,Jinan,China
  3. Clinical College, Shandong University of Traditional Chinese Medicine,Jinan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30901920), Shandong Province Natural Funded Projects (No. Q2008C17) and Shandong Province Traditional Chinese Medical Science and Technology Development Programs (No. 2009-038)
- DOI：10.1007/s11655-012-1273-7
  CLC：
- Published：2012，
  
  Published Online：20 October 2012，
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Sun, Zg., Lian, F., Jia, Q. et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation., Chin. J. Integr. Med. 18, 846–849 (2012). https://doi.org/10.1007/s11655-012-1273-7

Zhen-gao Sun, Fang Lian, Qing Jia, et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation. [J]. Chinese Journal of Integrative Medicine 18(11):846-849(2012)
Sun, Zg., Lian, F., Jia, Q. et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation., Chin. J. Integr. Med. 18, 846–849 (2012). https://doi.org/10.1007/s11655-012-1273-7 DOI：

Zhen-gao Sun, Fang Lian, Qing Jia, et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation. [J]. Chinese Journal of Integrative Medicine 18(11):846-849(2012) DOI： 10.1007/s11655-012-1273-7.

摘要

To investigate the effects of Er’zhi Tiangui Granule (二至天癸颗粒

ETG) on sequential expressions of integrinβ3 and its ligand osteopontin in the mouse endometrium during controlled ovarian hyperstimulation (COH) and implantation period. Seventy-five Mature female Kunming mice were randomly divided into 3 groups

a normal control group

a model group

and a treatment group administrated with ETG for 10 days

25 in each group. After mated with male mice

every 5 mice were sacrified in each group at the 0

2nd

4th

6th

and 8th days to take their endometrium. In-situ hybridization was used to detect the expressions of integrinβ3 and osteopontin in the endometrium. mRNA expressions of integrinβ3 and osteopontin in the endometrium during implantation period showed similar time sequence rules in the treatment group to those in the normal control group; the peak values of them were a little lower in the treatment group than the normal control without significant differences. In the model group

integrinβ3 mRNA expression was higher at the 2nd day

obviously lower at the 4th and 6th days

and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th

6th

and 8th days

compared with the normal control and the treatment groups (P<0.05

P<0.01). COH might influence the sequential expressions of integrinβ3 and its ligand osteopontin

bring forward the integrinβ3 expression peak

impact on the cooperation of integrinβ3 and osteopontin

so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrinβ3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.

Abstract

To investigate the effects of Er’zhi Tiangui Granule (二至天癸颗粒

a normal control group

a model group

and a treatment group administrated with ETG for 10 days

25 in each group. After mated with male mice

every 5 mice were sacrified in each group at the 0

2nd

4th

6th

integrinβ3 mRNA expression was higher at the 2nd day

obviously lower at the 4th and 6th days

and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th

6th

and 8th days

compared with the normal control and the treatment groups (P<0.05

P<0.01). COH might influence the sequential expressions of integrinβ3 and its ligand osteopontin

bring forward the integrinβ3 expression peak

impact on the cooperation of integrinβ3 and osteopontin

so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrinβ3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.

关键词

Kidney-tonifyingEr’zhi Tiangui Granulecontrolled ovarian hyperstimulationendometrial receptivityintegrinβ3osteopontinmouse

Keywords

Kidney-tonifyingEr’zhi Tiangui Granulecontrolled ovarian hyperstimulationendometrial receptivityintegrinβ3osteopontinmouse

references

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Ledee-Bataille N, Lapree-Delage G, Taupin JL, Dubanchet S, Frydman R, Chaouat G. Concentration of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation. Hum Reprod 2002;17:213–218.

Zhao LY, Xia T, Song WJ. Progress of Chinese and Western medicine treatment on endometrial receptivity during IVF. Chin J Integr Tradit West Med (Chin) 2011;31:1581–1584.

Wang YN, Song DR. Progress of study on Chinese medicine in improving endometrial receptivity. Chin J Integr Tradit West Med (Chin) 2009;29:666–668.

Wang MM, Hao CF, Bao HC, Zhang N, Ge L. Clinical efficacy of Chinese herbal retention enema combined with intrauterine douching for patients with endometritis. Chin J Integr Tradit West Med (Chin) 2011;31:639–642.

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Reproductive Medicine Center, Department of Obstetrics and Gynecology, General Hospital of Guangzhou Military Command, Liuhuaqiao Hospital

Department of General Practice, the Second Affiliated Hospital of Zhejiang University Medical College

Department of Allergy, the Second Affiliated Hospital of Zhejiang University Medical College

Department of Laboratory, the Second Affiliated Hospital of Zhejiang University Medical College

Department of Respiratory Medicine, the First People’s Hospital of Hangzhou

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