Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats

Xiao-yong Zheng; Ri-bao Wei; Suo-zhu Shi; Zhong Yin; Xiang-mei Chen

doi:10.1007/s11655-012-1295-1

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Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats

Updated：2021-08-27

- Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats
- Chinese Journal of Integrative Medicine Vol. 18, Issue 12, Pages: 918-924(2012)
- Affiliations：
  
  Nephropathy of Chinese PLA General Hospital, State Discipline and Key Laboratory of Kidney Disease (Chinese PLA General Hospital 2011DAV),Beijing,China,00088
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072914 and 81273968);Major Project Foundation of National Science and Technology (No. 2010ZX9102-204);and Traditional Chinese Medicine Research Grant for Military Organization (No. 10ZYZ255)
- DOI：10.1007/s11655-012-1295-1
  CLC：
- Published：2012，
  
  Published Online：13 December 2012，
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Zheng, Xy., Wei, Rb., Shi, Sz. et al. Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats., Chin. J. Integr. Med. 18, 918–924 (2012). https://doi.org/10.1007/s11655-012-1295-1

Xiao-yong Zheng, Ri-bao Wei, Suo-zhu Shi, et al. Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats. [J]. Chinese Journal of Integrative Medicine 18(12):918-924(2012)
Zheng, Xy., Wei, Rb., Shi, Sz. et al. Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats., Chin. J. Integr. Med. 18, 918–924 (2012). https://doi.org/10.1007/s11655-012-1295-1 DOI：

Xiao-yong Zheng, Ri-bao Wei, Suo-zhu Shi, et al. Effects of Fufang Shenhua Tablet (复方肾华片) on the expression of toll-like receptors during acute kidney injury induced by ischemia-reperfusion in rats. [J]. Chinese Journal of Integrative Medicine 18(12):918-924(2012) DOI： 10.1007/s11655-012-1295-1.

摘要

To investigate the impact of a traditional Chinese medicinal compound known as Fufang Shenhua Tablet (复方肾华片

SHP) on the expression of Toll-like receptors (TLRs) during renal ischemia-reperfusion injury (IRI)-induced acute kidney injury (AKI) in rats. A total of 28 Wistar rats were randomly divided into five groups: (1) pseudo-operation control group

(2) ischemia-reperfusion model group

(3) Astragaloside group

(4) high-dose SHP group

and (5) low-dose SHP group. There were four rats in the pseudo-operation group and six rats in each of the other groups. The accepted ischemia-reperfusion model was established after a 7-day gavage intervention

and pathological changes and renal function were observed

using an enzyme-linked immunosorbent assay (ELISA) to detect interleukin 8 (IL-8) and interferon gamma (IFN-γ) levels

as well as immunohistochemical staining to detect altered levels of TLR2 and TLR4 expression in renal tissue. After 24 h

renal pathological damage and the expression levels of serum creatinine (Scr)

IL-8

IFN-γ

TLR2

and TLR4 were significantly higher in the model group as compared with the pseudo-operation group (P<0.05). In addition

at 24 h the above indicators decreased significantly in the Astragaloside group

high-dose SHP group and low-dose SHP group as compared with the ischemia-reperfusion model group (P< 0.05). TLR2 and TLR4 expression levels were significantly reduced in the SHP treatment and Astragaloside group as compared with the pseudo-operation group (P<0.05). Further

the high-dose SHP group showed significantly less renal damage score and decreased levels of TLR expression than those of low-dose SHP group and Astragaloside group (all P<0.05). SHP can alleviate the renal structural and functional damage caused by IRI-induced AKI in rats by reducing the damage of renal pathology

which may reduce inflammatory cytokine levels by downregulating the expression of TLRs in renal tissue in a dose-dependent manner.

Abstract

To investigate the impact of a traditional Chinese medicinal compound known as Fufang Shenhua Tablet (复方肾华片

(2) ischemia-reperfusion model group

(3) Astragaloside group

(4) high-dose SHP group

and pathological changes and renal function were observed

using an enzyme-linked immunosorbent assay (ELISA) to detect interleukin 8 (IL-8) and interferon gamma (IFN-γ) levels

as well as immunohistochemical staining to detect altered levels of TLR2 and TLR4 expression in renal tissue. After 24 h

renal pathological damage and the expression levels of serum creatinine (Scr)

IL-8

IFN-γ

TLR2

and TLR4 were significantly higher in the model group as compared with the pseudo-operation group (P<0.05). In addition

at 24 h the above indicators decreased significantly in the Astragaloside group

which may reduce inflammatory cytokine levels by downregulating the expression of TLRs in renal tissue in a dose-dependent manner.

关键词

acute renal injuryischemia-reperfusionratsFufang Shenhua TabletToll-like receptor

Keywords

acute renal injuryischemia-reperfusionratsFufang Shenhua TabletToll-like receptor

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