Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke

Lu Liu; Ying Gao; Bin Ma

doi:10.1007/s11655-013-1194-8

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Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke

Updated：2021-08-27

- Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke
- Chinese Journal of Integrative Medicine Vol. 20, Issue 1, Pages: 11-18(2014)
- Affiliations：
  
  Department of Neurology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Major Foundation Research Development Plan (No. 2003CB517102);State Major Science and Technology Special Projects “Major Creation of New Drugs” (No. 2009ZX09502-028).
- DOI：10.1007/s11655-013-1194-8
  CLC：
- Published：2014，
  
  Published Online：25 March 2013，
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Liu, L., Gao, Y. & Ma, B. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke., Chin. J. Integr. Med. 20, 11–18 (2014). https://doi.org/10.1007/s11655-013-1194-8

Lu Liu, Ying Gao, Bin Ma. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke. [J]. Chinese Journal of Integrative Medicine 20(1):11-18(2014)
Liu, L., Gao, Y. & Ma, B. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke., Chin. J. Integr. Med. 20, 11–18 (2014). https://doi.org/10.1007/s11655-013-1194-8 DOI：

Lu Liu, Ying Gao, Bin Ma. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke. [J]. Chinese Journal of Integrative Medicine 20(1):11-18(2014) DOI： 10.1007/s11655-013-1194-8.

摘要

To investigate whether Chinese medicine (CM) syndrome is associated with particular molecular mechanism

we explored the correlation between CM syndrome and changes of intercellular adhesion molecule-1 (ICAM-1)

matrix metalloproteinase 9 (MMP-9) and heat shock protein 70 (HSP70) in patients with ischemic stroke

which were reported to play an important role in the inflammatory and apoptosis cascade. CM syndrome factors of 175 patients with ischemic stroke were assessed using Ischemic Stroke CM Syndrome Factor Diagnostic Scale (ISTSFDS). The patients were grouped according to the main syndrome factor combinations at different time points based on distribution probability of syndrome factor combinations. Blood levels of ICAM-1

MMP-9 and HSP70 were quantified by enzyme-linked immunosorbent assay. ICAM-1 expression was significantly higher in the internal-wind+phlegm-dampness+blood-stasis

phlegmdampness+ blood-stasis

internal-fire+phlegm-dampness+blood-stasis group than that in the blood-stasis+qideficiency group within 72 h from stroke onset (P <0.05); HSP70 expression was significantly lower in the phlegm-dampness+blood-stasis

internal-fire+phlegm-dampness+blood-stasis

blood-stasis group than that in the phlegm-dampness+blood-stasis+qi-deficiency group on the 7th day from stroke onset (P<0.05). Phlegm-dampness and blood-stasis exist through the whole process of ischemic stroke. An increased level of ICAM-1 and a reduced level of HSP70 reflect the pathological state of phlegm-stasis mutual binding. These results suggest that inflammation and apoptosis induced by cerebral vascular injury in the pathological processes of ischemic stroke are more prominent in the excess syndrome state like phlegm-dampness and blood-stasis.

Abstract

To investigate whether Chinese medicine (CM) syndrome is associated with particular molecular mechanism

we explored the correlation between CM syndrome and changes of intercellular adhesion molecule-1 (ICAM-1)

matrix metalloproteinase 9 (MMP-9) and heat shock protein 70 (HSP70) in patients with ischemic stroke

MMP-9 and HSP70 were quantified by enzyme-linked immunosorbent assay. ICAM-1 expression was significantly higher in the internal-wind+phlegm-dampness+blood-stasis

phlegmdampness+ blood-stasis

internal-fire+phlegm-dampness+blood-stasis

关键词

ischemic strokeChinese medicine syndromeintercellular adhesion molecule-1matrix metalloproteinase 9heat shock protein 70

Keywords

ischemic strokeChinese medicine syndromeintercellular adhesion molecule-1matrix metalloproteinase 9heat shock protein 70

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Division of Public Health Sciences, Fred Hutchinson Cancer Research Center

School of Public Health, Peking University Health Science Center, Peking University

Neurology Department of Peking University First Hospital, Peking University

Neurology Department of Peking University Third Hospital, Peking University

Department of Hospital Management, Peking University Health Science Center, Peking University

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