Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury

Yi-min Zhang; Yu-qing Zhang; Shao-bing Cheng; Sheng-xin Chen; Ai-lian Chen; Chun-zhi Tang

doi:10.1007/s11655-013-1353-6

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Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury

Updated：2021-08-27

- Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury
- Chinese Journal of Integrative Medicine Vol. 19, Issue 2, Pages: 132-136(2013)
- Affiliations：
  
  1. Jinan University,Guangzhou,China
  2. Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by Project of Natural Science Foundation of Guangdong (No. 9151063201000028) and Project of Science and Technology of Guangdong (No. 2011B031800284)
- DOI：10.1007/s11655-013-1353-6
  CLC：
- Published：2013，
  
  Published Online：31 January 2013，
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Zhang, Ym., Zhang, Yq., Cheng, Sb. et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury., Chin. J. Integr. Med. 19, 132–136 (2013). https://doi.org/10.1007/s11655-013-1353-6

Yi-min Zhang, Yu-qing Zhang, Shao-bing Cheng, et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury. [J]. Chinese Journal of Integrative Medicine 19(2):132-136(2013)
Zhang, Ym., Zhang, Yq., Cheng, Sb. et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury., Chin. J. Integr. Med. 19, 132–136 (2013). https://doi.org/10.1007/s11655-013-1353-6 DOI：

Yi-min Zhang, Yu-qing Zhang, Shao-bing Cheng, et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury. [J]. Chinese Journal of Integrative Medicine 19(2):132-136(2013) DOI： 10.1007/s11655-013-1353-6.

摘要

To observe the effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injuny. Thirty SD rats were randomly and equally allocated to the sham-operated

the model and the acupuncture groups. The traumatic brain injury model was established by the free drop method. For the rats in the acupuncture group

acupuncture was applied once a day for 7 days. Brain histotomy was carried out when treatments were completed. Immunohistochemical techniques were adopted to detect the cells that express nestin

neurofilament proteins (NF)-200 and glial fibrillary acidic proteins (GFAP)

the markers of neural stem cells

neurons

astrocytes respectively. Compared to the sham-operated group

the number of nestin-positive cells and NF-200-positive cells in brain tissues was decreased significantly in the model group (P < 0.01)

whereas the number of GFAP-positive cells was significantly increased P<0.01). Compared to the model group

the positive cells of nestin

NF-200

GFAP in brain tissues in the acupuncture group were increased obviously (P<0.01). Acupuncture can significantly increase the number of nestin-positive cells

NF-200-positive cells and GFAP-positive cells

indicating the significant increase of neural stem cells

neurons and astrocytes in number. Acupuncture can improve neuranagenesis by promoting the proliferation and differentiation of neural stem cells in brain tissues. This might be one of the mechanisms for acupuncture to treat traumatic brain injury and to promote the repair of nervous function.

Abstract

the model and the acupuncture groups. The traumatic brain injury model was established by the free drop method. For the rats in the acupuncture group

acupuncture was applied once a day for 7 days. Brain histotomy was carried out when treatments were completed. Immunohistochemical techniques were adopted to detect the cells that express nestin

neurofilament proteins (NF)-200 and glial fibrillary acidic proteins (GFAP)

the markers of neural stem cells

neurons

astrocytes respectively. Compared to the sham-operated group

the number of nestin-positive cells and NF-200-positive cells in brain tissues was decreased significantly in the model group (P < 0.01)

whereas the number of GFAP-positive cells was significantly increased P<0.01). Compared to the model group

the positive cells of nestin

NF-200

GFAP in brain tissues in the acupuncture group were increased obviously (P<0.01). Acupuncture can significantly increase the number of nestin-positive cells

NF-200-positive cells and GFAP-positive cells

indicating the significant increase of neural stem cells

关键词

traumatic brain injuryacupunctureneural stem cellsproliferation and differentiationrats

Keywords

traumatic brain injuryacupunctureneural stem cellsproliferation and differentiationrats

references

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Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University

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