Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice

Chang-gui Chen; Hui-ying Wang; Yu Dai; Jiao-li Wang; Wei-hua Xu

doi:10.1007/s11655-013-1410-1

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Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice

Updated：2021-08-27

- Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice
- Chinese Journal of Integrative Medicine Vol. 19, Issue 4, Pages: 282-288(2013)
- Affiliations：
  
  1. Department of General Practice, the Second Affiliated Hospital of Zhejiang University Medical College,Hangzhou,China
  2. Department of Allergy, the Second Affiliated Hospital of Zhejiang University Medical College,Hangzhou,China
  3. Department of Laboratory, the Second Affiliated Hospital of Zhejiang University Medical College,Hangzhou,China
  4. Department of Respiratory Medicine, the First People’s Hospital of Hangzhou,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30600266)
- DOI：10.1007/s11655-013-1410-1
  CLC：
- Published：2013，
  
  Published Online：15 January 2013，
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Chen, Cg., Wang, Hy., Dai, Y. et al. Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice., Chin. J. Integr. Med. 19, 282–288 (2013). https://doi.org/10.1007/s11655-013-1410-1

Chang-gui Chen, Hui-ying Wang, Yu Dai, et al. Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice. [J]. Chinese Journal of Integrative Medicine 19(4):282-288(2013)
Chen, Cg., Wang, Hy., Dai, Y. et al. Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice., Chin. J. Integr. Med. 19, 282–288 (2013). https://doi.org/10.1007/s11655-013-1410-1 DOI：

Chang-gui Chen, Hui-ying Wang, Yu Dai, et al. Tripterygium polyglycosid attenuates the established airway inflammation in asthmatic mice. [J]. Chinese Journal of Integrative Medicine 19(4):282-288(2013) DOI： 10.1007/s11655-013-1410-1.

摘要

To investigate the effect of Tripterygium polyglycosid on establishing airway eosinophil infiltration and related airway hyperresponsiveness of asthmatic mice. A mature murine asthmatic model was made with ovabulmin sensitized and challenged C57BL/6 mice. Forty mice were divided into four groups with 10 mice in each group: mice sensitized and challenged with saline (WS group)

mice sensitized and challenged with ovalbumin (WO group)

mice sensitized and challenged with ovalbumin and treated with Tripterygium polyglycosid (TP group) and Dexamethasone (DXM group). The mice were intraperitoneally injected with 20 μg chicken ovabulmin emulsified in injected alum on days 0 and 14

then were challenged with an aerosol generated from 1% ovabulmin on days 24

25 and 26. Tripterygium polyglycosid was injected intraperitoneally at 50 mg/kg on days 25

26 and 27 after ovabulmin challenge. Dexamethasone was administrated to mice at 2 mg/kg on day 21

23 before ovabulmin challenge. The airway hyperresponsiveness

mucus production

eosinophils in parabronchial area and bronchoalveolar lavage fluid and the level of interleukin-5

granulo-macrophage clone stimulating factor in bronchoalveolar lavage fluid were measured as indexes of inflammation. Tripterygium polyglycosid treatment after ovabulmin challenge completely inhibited eosinophil infiltration in bronchoalveolar lavage fluid [(0.63±0.34)×104 vs. (75.0±14.8)×104

P<0.05] and the peribrochial area (12.60±3.48 mm2 vs. 379.0±119.3 mm2

P<0.05)

mucus overproduction in airway (2.8±1.7 vs. 7.1±5.6

P<0.05)

and increased interleukin-5 levels in bronchoalveolar lavage fluid (28.8±2.8 pg/mL vs. 7.5±3.5 pg/mL

P<0.05). Meanwhile

Tripterygium polyglycosid treatment after ovabulmin challenge also partially inhibited airway hyperresponsiveness. The level of granulo-macrophage clone stimulating factor in bronchoalveolar lavage fluid didn’t change with drugs intervention. The administration of Tripterygium polyglycosid could inhibit the established airway inflammation and reduce the airway hyperresponsiveness of allergic asthmatic mice. It provides a possible alternative therapeutic for asthma.

Abstract

mice sensitized and challenged with ovalbumin (WO group)

then were challenged with an aerosol generated from 1% ovabulmin on days 24

25 and 26. Tripterygium polyglycosid was injected intraperitoneally at 50 mg/kg on days 25

26 and 27 after ovabulmin challenge. Dexamethasone was administrated to mice at 2 mg/kg on day 21

23 before ovabulmin challenge. The airway hyperresponsiveness

mucus production

eosinophils in parabronchial area and bronchoalveolar lavage fluid and the level of interleukin-5

P<0.05] and the peribrochial area (12.60±3.48 mm2 vs. 379.0±119.3 mm2

P<0.05)

mucus overproduction in airway (2.8±1.7 vs. 7.1±5.6

P<0.05)

and increased interleukin-5 levels in bronchoalveolar lavage fluid (28.8±2.8 pg/mL vs. 7.5±3.5 pg/mL

P<0.05). Meanwhile

关键词

Tripterygium polyglycosidAsthmamouseairway hyperresponsivenesseosinophils

Keywords

Tripterygium polyglycosidAsthmamouseairway hyperresponsivenesseosinophils

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Basic Medicine Research Institution, Shandong Academy of Medical Sciences

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Center for Chinese Medicine for Allergy and Asthma, Jaffe Food Allergy Institute, Pediatric Department, Division of Allergy and Immunology, The Mount Sinai School of Medicine

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