Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure

Si-dao Zheng; Hong-jin Wu; Shao-ping Yu; Jian-xun Ren; Wei-wei Duo; Zeng-chun Ma; Yue Gao; Sheng-qi Wang; Yu-na Liu

doi:10.1007/s11655-013-1440-8

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Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure

Updated：2021-08-27

- Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure
- Chinese Journal of Integrative Medicine Vol. 21, Issue 1, Pages: 22-28(2015)
- Affiliations：
  
  1. Beijing Hospital of Integrated Traditional Chinese and Western Medicine,Beijing,China
  2. Beijing Institute of Radiation Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the Science and Technology Fund of the Beijing Bureau of Traditional Chinese Medicine (No. JJ2001-37) and the National Nature Science Foundation of China (No. 81173367)
- DOI：10.1007/s11655-013-1440-8
  CLC：
- Published：2015，
  
  Published Online：15 March 2013，
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Zheng, Sd., Wu, Hj., Yu, Sp. et al. Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure., Chin. J. Integr. Med. 21, 22–28 (2015). https://doi.org/10.1007/s11655-013-1440-8

Si-dao Zheng, Hong-jin Wu, Shao-ping Yu, et al. Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure. [J]. Chinese Journal of Integrative Medicine 21(1):22-28(2015)
Zheng, Sd., Wu, Hj., Yu, Sp. et al. Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure., Chin. J. Integr. Med. 21, 22–28 (2015). https://doi.org/10.1007/s11655-013-1440-8 DOI：

Si-dao Zheng, Hong-jin Wu, Shao-ping Yu, et al. Shenfu Injection (参附注射液) suppresses inflammation by targeting haptoglobin and pentraxin 3 in rats with chronic ischemic heart failure. [J]. Chinese Journal of Integrative Medicine 21(1):22-28(2015) DOI： 10.1007/s11655-013-1440-8.

摘要

To investigate the regulatory effects of Shenfu Injection (SFI

参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF). Forty-five healthy Wistar rats were randomized into three groups: sham

heart failure (model) and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation

animals in the sham group and the model group received saline (6.2 mL/kg/d)

while animals in the SFI group received SFI (6.2 mL/kg d) intraperitoneally. Four weeks later

cardiac hemodynamic parameters were measured via the carotid route. The expression of serum proteins was analyzed by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS). Recording of hemodynamic parameters showed that left ventricular systolic pressure (LVSP)

maximum rate of left ventricular pressure (+dp/dtmax) rise

and maximum rate of left ventricular pressure (−dp/dtmax) decrease

while the left ventricular end diastolic pressure (LVEDP) rose in the model group compared to those in the sham group (P <0.05). The results of the MALDI-TOF MS indicated that haptoglobin (HP)

pentraxin 3 (PTX3) and alpha-1-antitrypsin were up-regulated

while serum albumin and 40S ribosomal protein were down-regulated in the model group (P <0.05). Compared with the model group

LVSP

+dp/dtmax and −dp/dtmax were higher

while LVEDP was lower in the SFI group (P<0.05). Expression levels of HP and PTX3 were lower than in the model group (P<0.05). SFI could improve hemodynamic function and decrease inflammatory reactions in the pathophysiology of CHF. The serum proteins HP and PTX3 could be potential biomarkers for chronic ischemic heart failure

and they could also be the serum protein targets of SFI.

Abstract

To investigate the regulatory effects of Shenfu Injection (SFI

参附注射液) on hemodynamic parameters and serum proteins in rats with post-infarction chronic heart failure (CHF). Forty-five healthy Wistar rats were randomized into three groups: sham

heart failure (model) and SFI group. The CHF was induced by left coronary artery ligation. Seven days after the surgical operation

animals in the sham group and the model group received saline (6.2 mL/kg/d)

while animals in the SFI group received SFI (6.2 mL/kg d) intraperitoneally. Four weeks later

maximum rate of left ventricular pressure (+dp/dtmax) rise

and maximum rate of left ventricular pressure (−dp/dtmax) decrease

while the left ventricular end diastolic pressure (LVEDP) rose in the model group compared to those in the sham group (P <0.05). The results of the MALDI-TOF MS indicated that haptoglobin (HP)

pentraxin 3 (PTX3) and alpha-1-antitrypsin were up-regulated

while serum albumin and 40S ribosomal protein were down-regulated in the model group (P <0.05). Compared with the model group

LVSP

+dp/dtmax and −dp/dtmax were higher

and they could also be the serum protein targets of SFI.

关键词

Shenfu Injectionchronic heart failureproteomicshaptoglobinpentraxin 3

Keywords

Shenfu Injectionchronic heart failureproteomicshaptoglobinpentraxin 3

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