Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis

Qing-bo Zhou; Cheng-zhu Duan; Qing Jia; Ping Liu; Lu-yang Li

doi:10.1007/s11655-013-1441-7

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Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis

Updated：2021-08-27

- Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis
- Chinese Journal of Integrative Medicine Vol. 20, Issue 2, Pages: 116-122(2014)
- Affiliations：
  
  1. Department of Neurology, The Second Hospital, Shandong University,Jinan,China
  2. Shandong University School of Medicine,Jinan,China
  3. Department of Experimental Pathology, Institute of Basic Medicine, Shandong Academy of Medical Sciences,Jinan,China
  4. China Institute of Pharmacology, College of Medicine, Shandong University,Jinan,China
  5. Department of Gerontology, Qilu Hospital, Shandong University,Jinan,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81072916), Shandong Science and Technique Foundation (No. 2005GG3202062), Shandong Traditional Chinese Medicine Administration Fund Program (No.2009-160) and Free Exploration Program of Shandong University (No.2009TS009)
- DOI：10.1007/s11655-013-1441-7
  CLC：
- Published：2014，
  
  Published Online：16 March 2013，
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Zhou, Qb., Duan, Cz., Jia, Q. et al. Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis., Chin. J. Integr. Med. 20, 116–122 (2014). https://doi.org/10.1007/s11655-013-1441-7

Qing-bo Zhou, Cheng-zhu Duan, Qing Jia, et al. Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis. [J]. Chinese Journal of Integrative Medicine 20(2):116-122(2014)
Zhou, Qb., Duan, Cz., Jia, Q. et al. Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis., Chin. J. Integr. Med. 20, 116–122 (2014). https://doi.org/10.1007/s11655-013-1441-7 DOI：

Qing-bo Zhou, Cheng-zhu Duan, Qing Jia, et al. Baicalin attenuates focal cerebral ischemic reperfusion injury by inhibition of protease-activated receptor-1 and apoptosis. [J]. Chinese Journal of Integrative Medicine 20(2):116-122(2014) DOI： 10.1007/s11655-013-1441-7.

摘要

To investigate the neuro-protective effects of baicalin in Wistar rats with focal cerebral ischemic reperfusion injury. Ninety adult male Wistar rats weighing 320–350 g were randomly divided into the following groups (n=5): (a) sham control group; (b) vehicle group

subjected to middle cerebral artery occlusion and received vehicle intraperitoneally; (c-e) baicalin groups

which were subjected to the middle cerebral artery occlusion and treated with baicalin 25

50 and 100 mg/kg

respectively. The neurological scores were determined at postoperative 1

3 and 7 d after the treatment. The expression of protease-activated receptor-1 (PAR-1)

PAR-1 mRNA and Caspase-3 were determined using Western blot

reverse transcription polymerase chain reaction (RTPCR) analysis and immunohistochemistry

respectively. Significant decrease was noted in the neurological score in the baicalin group compared with that of the vehicle group (P<0.01). Additionally

down-regulation of PAR-1 mRNA

PAR-1 and Caspase-3 was observed in the baicalin groups compared with those obtained from the vehicle group (P<0.01). Compared with the low-dose baicalin group (25 mg/kg)

remarkable decrease was noted in neurological score

and the expression of PAR-1 mRNA

PAR-1 as well as Caspase-3 in the high-dose group (P<0.05). Baicalin showed neuro-protective effects in focal cerebral ischemic reperfusion injury through inhibiting the expression of PAR-1 and apoptosis.

Abstract

subjected to middle cerebral artery occlusion and received vehicle intraperitoneally; (c-e) baicalin groups

which were subjected to the middle cerebral artery occlusion and treated with baicalin 25

50 and 100 mg/kg

respectively. The neurological scores were determined at postoperative 1

3 and 7 d after the treatment. The expression of protease-activated receptor-1 (PAR-1)

PAR-1 mRNA and Caspase-3 were determined using Western blot

reverse transcription polymerase chain reaction (RTPCR) analysis and immunohistochemistry

respectively. Significant decrease was noted in the neurological score in the baicalin group compared with that of the vehicle group (P<0.01). Additionally

down-regulation of PAR-1 mRNA

PAR-1 and Caspase-3 was observed in the baicalin groups compared with those obtained from the vehicle group (P<0.01). Compared with the low-dose baicalin group (25 mg/kg)

remarkable decrease was noted in neurological score

and the expression of PAR-1 mRNA

关键词

Baicalincerebral ischemia-reperfusionprotease-activated receptor-1caspase-3neuroprotection

Keywords

Baicalincerebral ischemia-reperfusionprotease-activated receptor-1caspase-3neuroprotection

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