Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Shokoofe Noori; Zuhair. M. Hassan; Vida Farsam

doi:10.1007/s11655-013-1454-2

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Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Updated：2021-08-27

- Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line
- Chinese Journal of Integrative Medicine Vol. 20, Issue 8, Pages: 618-623(2014)
- Affiliations：
  
  1. Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran
  2. Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran,Iran
- Author bio：
- Funds：
- DOI：10.1007/s11655-013-1454-2
  CLC：
- Published：2014，
  
  Published Online：15 June 2013，
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Noori, S., Hassan, Z.M. & Farsam, V. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line., Chin. J. Integr. Med. 20, 618–623 (2014). https://doi.org/10.1007/s11655-013-1454-2

Shokoofe Noori, Zuhair. M. Hassan, Vida Farsam. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line. [J]. Chinese Journal of Integrative Medicine 20(8):618-623(2014)
Noori, S., Hassan, Z.M. & Farsam, V. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line., Chin. J. Integr. Med. 20, 618–623 (2014). https://doi.org/10.1007/s11655-013-1454-2 DOI：

Shokoofe Noori, Zuhair. M. Hassan, Vida Farsam. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line. [J]. Chinese Journal of Integrative Medicine 20(8):618-623(2014) DOI： 10.1007/s11655-013-1454-2.

摘要

To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. Column chromatography

thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS). The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover

Artemisinin

in a dose-dependent manner

could significantly increase the level of ROS. Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

Abstract

To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. Column chromatography

Artemisinin

in a dose-dependent manner

could significantly increase the level of ROS. Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

关键词

Artemisininendoperoxide bridgeapoptosisreactive oxygen species

Keywords

Artemisininendoperoxide bridgeapoptosisreactive oxygen species

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