Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats

Jie-kun Luo; Hua-jun Zhou; Jing Wu; Tao Tang; Qing-hua Liang

doi:10.1007/s11655-013-1458-y

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Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats

Updated：2021-08-27

- Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats
- Chinese Journal of Integrative Medicine Vol. 19, Issue 5, Pages: 367-373(2013)
- Affiliations：
  
  1. Institute of Integrative Medicine, Xiangya Hospital, Central South University,Hunan,Changsha,China
  2. Institute of Neurology, Xiangya Hospital, Central South University,Hunan,Changsha,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30873221), Project for New Century Excellent Talents (No. NCET-11-0522), Hunan Provincial Natural Science Foundation (No. 08JJ3077 and No. 10JJ2023), and the program of the Key State Administration of Traditional Chinese Medicine (SATCM) Lab of Chinese Gan (SATCM 2012-46)
- DOI：10.1007/s11655-013-1458-y
  CLC：
- Published：2013，
  
  Published Online：15 May 2013，
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Luo, Jk., Zhou, Hj., Wu, J. et al. Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats., Chin. J. Integr. Med. 19, 367–373 (2013). https://doi.org/10.1007/s11655-013-1458-y

Jie-kun Luo, Hua-jun Zhou, Jing Wu, et al. Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats. [J]. Chinese Journal of Integrative Medicine 19(5):367-373(2013)
Luo, Jk., Zhou, Hj., Wu, J. et al. Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats., Chin. J. Integr. Med. 19, 367–373 (2013). https://doi.org/10.1007/s11655-013-1458-y DOI：

Jie-kun Luo, Hua-jun Zhou, Jing Wu, et al. Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats. [J]. Chinese Journal of Integrative Medicine 19(5):367-373(2013) DOI： 10.1007/s11655-013-1458-y.

摘要

To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats. Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these

one group was not further treated

the second group underwent Zusanli (ST36)-acupuncture

and the third group underwent non-acupoint acupuncture. The fourth group underwent sham operations. Acupuncture was performed by stimulation with electrical needles at frequencies of 2–20 Hz for 30 min per day. Angiogenesis on days 3

7 and 14 was assessed by double immunolabeling

and expression of HIF-1α was evaluated by immunohistochemistry

quantitative real time reverse transcription-polymerase chain reaction and Western blotting. 5-Bromo-2-deoxyuridine (BrdU)-labeled nuclei in cerebral endothelial cells (ECs) resided around the hematoma and the labeling peaked from 7 to 14 days (P<0.01). HIF-1α positive microvessels with a dilated outline were detected in perihematomal tissues after ICH

with the vessels extending into the clot from the surrounding area beginning on day 7. Following ICH

HIF-1α protein levels increased (P<0.05)

but HIF-1α mRNA levels did not change. Electro-acupuncture at the Zusanli (ST36) acupoint increased BrdU-labeled nuclei in cerebral ECs (P<0.05) and up-regulated the expression of HIF-1α protein (P<0.05)

but had little effect on the spatial distribution of HIF-1α or on HIF-1α mRNA levels. Conclusions: Electro-acupuncture treatment at the Zusanli (ST36) acupoint may accelerate ICH-induced angiogenesis by up-regulating HIF-1α protein

and may enhance recovery following hemorrhagic cerebral injury.

Abstract

one group was not further treated

the second group underwent Zusanli (ST36)-acupuncture

7 and 14 was assessed by double immunolabeling

and expression of HIF-1α was evaluated by immunohistochemistry

with the vessels extending into the clot from the surrounding area beginning on day 7. Following ICH

HIF-1α protein levels increased (P<0.05)

and may enhance recovery following hemorrhagic cerebral injury.

关键词

electro-acupunctureZusanli acupointintracerebral hemorrhageangiogenesishypoxiainducible factor-1α

Keywords

electro-acupunctureZusanli acupointintracerebral hemorrhageangiogenesishypoxiainducible factor-1α

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