Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release

Jin-yan Zhang; Yong-xiao Cao; Wei-liang Weng; Yi-kui Li; Le Zhao

doi:10.1007/s11655-013-1505-8

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Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release

Updated：2021-08-27

- Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release
- Chinese Journal of Integrative Medicine Vol. 19, Issue 7, Pages: 510-516(2013)
- Affiliations：
  
  1. Experiment and Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Department of Pharmacology, College of Medicine, Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30801531)
- DOI：10.1007/s11655-013-1505-8
  CLC：
- Published：2013，
  
  Published Online：2 July 2013，
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Zhang, Jy., Cao, Yx., Weng, Wl. et al. Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release., Chin. J. Integr. Med. 19, 510–516 (2013). https://doi.org/10.1007/s11655-013-1505-8

Jin-yan Zhang, Yong-xiao Cao, Wei-liang Weng, et al. Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release. [J]. Chinese Journal of Integrative Medicine 19(7):510-516(2013)
Zhang, Jy., Cao, Yx., Weng, Wl. et al. Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release., Chin. J. Integr. Med. 19, 510–516 (2013). https://doi.org/10.1007/s11655-013-1505-8 DOI：

Jin-yan Zhang, Yong-xiao Cao, Wei-liang Weng, et al. Paeonol induces vasodilatation in rat mesenteric artery via inhibiting extracellular Ca2+ influx and intracellular Ca2+ release. [J]. Chinese Journal of Integrative Medicine 19(7):510-516(2013) DOI： 10.1007/s11655-013-1505-8.

摘要

To investigate the vasodilative effect of paeonol in rat mesenteric artery and the mechanisms responsible for it. Rats were anaesthetized and sacrificed. The superior mesenteric artery was removed

dissected free of adherent tissue and cut into 2.0 mm long cylindrical segments. Isometric tension of artery rings was recorded by a myograph system in vitro. Concentration-relaxation curves of paeonol (17.8 μ mol/L to 3.16 mmol/L) were recorded on artery rings precontracted by potassium chloride (KCl) and concentration-contraction curves of KCl

5-hydroxytryptamine (5-HT)

noradrenaline (NA) or calcium chloride (CaCl2) were recorded in the presence of paeonol (10−4.5

10−3.8

10−3.5 mol/L) respectively. And also

concentrationrelaxation curves of paeonol were recorded in the presence of different potassium channel inhibitors and propranolol on rings precontracted with KCl respectively. To investigate the role of intracellular Ca2+ release from Ca2+ store

the contraction induced by NA (100 μ mol/L) and CaCl2 (2 mmol/L) in Ca2+ free medium was observed in the presence of paeonol respectively. Paeonol relaxed artery rings precontracted by KCl in a concentration-dependent manner and the vasodilatation effect was not affected by endothelium denudation. Paeonol significant decreased the maximum contractions (Emax) induced by KCl

CaCl2

NA and 5-HT

as well as Emax induced by NA and CaCl2 in Ca2+ -free medium

suggesting that paeonol dilated the artery via inhibiting the extracellular Ca2+ influx mediated by voltage-dependent calcium channel

and receptor-mediated Ca2+-influx and release. Moreover

none of glibenclamide

tetraethylammonium

barium chlorded and propranolol affected the paeonol-induced vasodilatation

indicating that the vasodilatation was not contributed to ATP sensitive potassium channel

calcium-activated potassium channel

inwardly rectifying potassium channel

and β-adrenoceptor. Paeonol induces non-endothelium dependent-vasodilatation in rat mesenteric artery via inhibiting voltage-dependent calcium channel-mediated extracellular Ca2+ influx and receptor-mediated Ca2+ influx and release.

Abstract

To investigate the vasodilative effect of paeonol in rat mesenteric artery and the mechanisms responsible for it. Rats were anaesthetized and sacrificed. The superior mesenteric artery was removed

5-hydroxytryptamine (5-HT)

noradrenaline (NA) or calcium chloride (CaCl2) were recorded in the presence of paeonol (10−4.5

10−3.8

10−3.5 mol/L) respectively. And also

CaCl2

NA and 5-HT

as well as Emax induced by NA and CaCl2 in Ca2+ -free medium

suggesting that paeonol dilated the artery via inhibiting the extracellular Ca2+ influx mediated by voltage-dependent calcium channel

and receptor-mediated Ca2+-influx and release. Moreover

none of glibenclamide

tetraethylammonium

barium chlorded and propranolol affected the paeonol-induced vasodilatation

indicating that the vasodilatation was not contributed to ATP sensitive potassium channel

calcium-activated potassium channel

inwardly rectifying potassium channel

关键词

paeonolvasodilatationcontractionrat mesenteric arterycalcium

Keywords

paeonolvasodilatationcontractionrat mesenteric arterycalcium

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