Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration

Pu-yan Wang; Xiao-chun Liang; Hong Zhang; Li Zhao; Qing Sun; Wen-zhi Huang; Bo-wu Li

doi:10.1007/s11655-013-1506-7

Your Location：

Home >

Browse articles >

Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration

Updated：2021-08-27

- Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration
- Chinese Journal of Integrative Medicine Vol. 19, Issue 7, Pages: 517-523(2013)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences,Beijing,China
  2. Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by Beijing Natural Science Foundation (No. 7082077);The author is now working in the Department of Traditional Chinese Medicine, 306th Hospital of People’s Liberation Army, Beijing (100101), China
- DOI：10.1007/s11655-013-1506-7
  CLC：
- Published：2013，
  
  Published Online：2 July 2013，
Scan for full text
Wang, Py., Liang, Xc., Zhang, H. et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration., Chin. J. Integr. Med. 19, 517–523 (2013). https://doi.org/10.1007/s11655-013-1506-7

Pu-yan Wang, Xiao-chun Liang, Hong Zhang, et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration. [J]. Chinese Journal of Integrative Medicine 19(7):517-523(2013)
Wang, Py., Liang, Xc., Zhang, H. et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration., Chin. J. Integr. Med. 19, 517–523 (2013). https://doi.org/10.1007/s11655-013-1506-7 DOI：

Pu-yan Wang, Xiao-chun Liang, Hong Zhang, et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration. [J]. Chinese Journal of Integrative Medicine 19(7):517-523(2013) DOI： 10.1007/s11655-013-1506-7.

摘要

To evaluate the effect of serum containing Jinmaitong Capsule (筋脉通胶囊

JMT) on apoptosis of Schwann cells (SCs) that are cultured in high glucose at the cellular and molecular levels. SCs were cultured in Dulbecco’s modified Eagle’s medium (control group)

high glucose (50 mmol/L) medium supplemented with 20% rat serum (HG group)

and 50 mmol/L glucose medium supplemented with serum containing JMT (JMT group). SC apoptosis was detected using a terminal deoxynucleotidyl transferase dUTP nick end labeling kit. The expression of Bcl-2 and the caspase-3 p20 subunit in SCs were detected by realtime fluorogenic quantitative polymerase chain reaction and confocal laser scanning microscopy

respectively. No apoptosis was detected in SCs that were cultured in the control group. The percentage of apoptosis of SCs cultured in the HG group was much higher than that in the control group. The apoptosis of SCs in the JMT group was lower than that in the HG group. Fluorescence intensity of Bcl-2 and the expression of Bcl-2 mRNA in SCs that were cultured in the HG group were much lower than those in the control group and much higher than those in the JMT group (P<0.01). The fluorescence intensity of caspase-3 p20 and the expression of caspase-3 p20 mRNA in SCs that were cultured in the HG group were much higher than those in the control group (P<0.01)

and they were remarkably lower in the JMT group (P<0.01). JMT effectively prevents SC apoptosis that is induced by high glucose. This effect may be because of increased expression of Bcl-2 mRNA and protein and decreased expression of caspase-3 p20 mRNA and protein.

Abstract

To evaluate the effect of serum containing Jinmaitong Capsule (筋脉通胶囊

JMT) on apoptosis of Schwann cells (SCs) that are cultured in high glucose at the cellular and molecular levels. SCs were cultured in Dulbecco’s modified Eagle’s medium (control group)

high glucose (50 mmol/L) medium supplemented with 20% rat serum (HG group)

关键词

Jinmaitong Capsuleapoptosishigh glucoseSchwann cells

Keywords

Jinmaitong Capsuleapoptosishigh glucoseSchwann cells

references

Edwards JL, Vincent AM, Cheng HT, Feldman EL. Diabetic neuropathy: mechanisms to management. Pharmacol Ther 2008;120:1–34.

Investigation Group for Chronic Diabetic Complications. A nationwide retrospective analysis on chronic diabetic complications and related macrovascular diseases of inpatient with diabetes during 1991–2000. Acta Acad Med Sin 2002;24:447–451.

Liang XC, Cui LY, Guo SS, Zhang KJ, Hao WX, Jia L, et al. Clinical study on Jinmaitong Composita on diabetic peripheral neuropathy. Chin J Integr Med 2001;7:103–106.

Qu L, Liang XC, Zhang H, Wu QL, Sun LQ, Gu P. Effect of Jinmaitong with medicated serum on proliferation of rat SCs cultured in high glucose medium. Chin Integr Med 2008;14:293–297.

Mathew CE, Berdanier CD. Noninsulin-dependent diabetes mellitus as a mitochondrial genomic disease. Proc Soc Exper Biol Med 1998;219:97–108.

Schapira AH. Mitochondrial dysfunction in neurodegenerative disorders. Biochim Biophys Acta 1998;1366:225–233.

Brownlee M. Biochemistry and molecular cell of diabetic complications. Nature 2001;414:813–820.

Peng YQ, Ji YL, Jiang ZP, Yu XQ, Du Y. Effects of inhibition of NAD(P)H oxidase expression and activity in diabetic nephropathy. J Sun Yat-sen Univ Med Sci (Chin) 2004;25:245–248,263.

Han Z, Hendrickson EA, Bremner TA, Wyche JH. A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro. J Biological Chem 1997;272:13432–13436.

Coppey LJ, Gellett JS, Davidson EP, Yorek MA. Preventing superoxide formation in epineurial arterioles of the sciatic nerve from diabetic rats restores endothelium-dependent vasodilation. Free Radic Res 2003;37:33–40.

Brownlee M. The pathobiology of diabetic complications: a unifying methanism. Diabeties 2005;54:1615–1625.

Jakobsen J, Lundbak K. Neuropathy in experimental diabetes: an animal model. Br Med J 1976;31(6030):278–279.

Chokroverty S, Seiden D, Navidad P, Cody R. Distal axonopathy in streptozotocin diabetes in rats. Experientia 1988;44:444–446.

Forman LJ, Estilow S, Lewis M, Lewis M, Vasilenko P. Streptozotocin diabetes alters immunoreactive β-endorphin levels and pain perception after 8wk in female rats. Diabetes 1986;35:1309–1313.

FULL TEXT LINK

More>

Views

1195

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium

Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells

The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells

Related Author

No data

Related Institution

Cell Center, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences

Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran

Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran

Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan

Department of Nursing, Min-Hwei College of Health Care Management, Tainan

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰