Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方)

Wei Qu; Zhao-shun Jiang; Chun-yang Zhang; Jun-jie Zou; Liang-liang Sun; Yong-quan Shi; Zhi-min Liu

doi:10.1007/s11655-013-1507-6

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Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方)

Updated：2021-08-27

- Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方)
- Chinese Journal of Integrative Medicine Vol. 19, Issue 7, Pages: 524-531(2013)
- Affiliations：
  
  1. Department of Endocrinology, General Military Hospital of Jinan Military Region,Jinan,China
  2. Department of Endocrinology, Tongji Hospital,Shanghai,China
  3. Department of Endocrinology, Changzheng Hospital, Second Military Medical University,Shanghai,China
- Author bio：
- Funds：
  
  Supported by National Program on Key Basic Research Projects (973 Program, No. 2005CB523304);These authors contributed equally to this work
- DOI：10.1007/s11655-013-1507-6
  CLC：
- Published：2013，
  
  Published Online：2 July 2013，
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Qu, W., Jiang, Zs., Zhang, Cy. et al. Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方)., Chin. J. Integr. Med. 19, 524–531 (2013). https://doi.org/10.1007/s11655-013-1507-6

Wei Qu, Zhao-shun Jiang, Chun-yang Zhang, et al. Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方). [J]. Chinese Journal of Integrative Medicine 19(7):524-531(2013)
Qu, W., Jiang, Zs., Zhang, Cy. et al. Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方)., Chin. J. Integr. Med. 19, 524–531 (2013). https://doi.org/10.1007/s11655-013-1507-6 DOI：

Wei Qu, Zhao-shun Jiang, Chun-yang Zhang, et al. Regulation of C-type natriuretic peptides and natriuretic peptide receptor-B expression in diabetic rats renal treated by Tongluo Recipe (通络方). [J]. Chinese Journal of Integrative Medicine 19(7):524-531(2013) DOI： 10.1007/s11655-013-1507-6.

摘要

To investigate the expression of C-type natriuretic peptides (CNP) and natriuretic peptide receptor-B (NPR-B) receptor in diabetic rats renal cortex

and the regulation by Tongluo Recipe (通络方

TLR). Sixty male SD rats were divided into 3 groups: the normal control group

diabetic model group and diabetic TLR group. Each group was further divided into two subgroups of ten in each

according to 4-week or 12-week observation period. Streptozotocin (STZ)-induced diabetic rats were treated with TLR (1.0 g·kg−1·d−1) for 4 and 12 weeks

respectively. (1) The essential information was collected for comparing renal mass

serum creatinine and 24 h urine albumen on each group was calculated. (2) CNP mRNA and NPR-B mRNA were detected by realtime-polymerase chain reaction (PCR) on rats renal cortex. (3) Concentration of CNP on renal cortex or serum were analyzed by enzyme-linked immunosorbent assay (ELISA). (4) Pathological evaluation and NPR-B immunostaining for renal tissue were also performed. (1) CNP and NPR-B mRNA levels were detected in each treated or untreated group

with slight elevated in untreated diabetes rats administrated with STZ after 4-week and CNP mRNA level remarkable elevated at 39.21 times higher than normal control group after 12 weeks

but NPR-B mRNA level showed a remarkably down-regulation at 98.07% after 12 weeks. CNP mRNA of TLR-treated group was also elevated after 12-week treatment

but less than untreated group. (2) Concentrations of CNP in renal cortex were obviously increased in treated or untreated diabetes rats

within these groups the treatment of TLR was found more significantly on prompting CNP concentration. Comparing to normal group

serum concentrations of CNP were also increased in treated or untreated diabetic groups

but there was no difference between these diabetic groups. (3) Renal lesions like glomerular volume increased are observed mostly in the relative early stage after 4 weeks. Although TLR treated group had no significant difference in their glomerular volume

the degrees of injury of glomerulus were ameliorated

as well as the NPR-B immunostaining enhanced in glomerulus. Weakly positive immunostaining of NPR-B are observed in glomerulus of normal control

and negative in glomerulus of untreated diabetes rats administrated with STZ after 12 weeks

whereas TLR-treatment groups showed a little enhancement. CNP and NPR-B showed different characteristic on renal cortex at different pathological period in diabetes rats

and TLR regulated their expression.

Abstract