Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury

Wei-qian Tian; Yong G. Peng; Su-yang Cui; Feng-zhen Yao; Bao-gui Li

doi:10.1007/s11655-013-1512-9

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Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury

Updated：2021-08-27

- Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury
- Chinese Journal of Integrative Medicine Vol. 21, Issue 8, Pages: 618-623(2015)
- Affiliations：
  
  1. Department of Anesthesiology, Affiliated Hospital of Nanjing Medical University of Traditional Chinese Medicine,Nanjing,China
  2. Department of Anesthesiology, Shands Hospital at the University of Florida, Gainesville,New York,USA
- Author bio：
- Funds：
- DOI：10.1007/s11655-013-1512-9
  CLC：
- Published：2015，
  
  Published Online：3 September 2013，
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Tian, Wq., Peng, Y.G., Cui, Sy. et al. Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury., Chin. J. Integr. Med. 21, 618–623 (2015). https://doi.org/10.1007/s11655-013-1512-9

Wei-qian Tian, Yong G. Peng, Su-yang Cui, et al. Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury. [J]. Chinese Journal of Integrative Medicine 21(8):618-623(2015)
Tian, Wq., Peng, Y.G., Cui, Sy. et al. Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury., Chin. J. Integr. Med. 21, 618–623 (2015). https://doi.org/10.1007/s11655-013-1512-9 DOI：

Wei-qian Tian, Yong G. Peng, Su-yang Cui, et al. Effects of electroacupuncture of different intensities on energy metabolism of mitochondria of brain cells in rats with cerebral ischemia-reperfusion injury. [J]. Chinese Journal of Integrative Medicine 21(8):618-623(2015) DOI： 10.1007/s11655-013-1512-9.

摘要

To observe the effects of electroacupuncture (EA) of different intensities on lactate dehydrogernase (LDH)

succinate dehydrogenase (SDH) and ATPase in brain tissue of rats with cerebral ischemia-reperfusion injury (CI/R). Forty male SD rats were uniformly randomized into sham operation group (group A)

CI/R group (group B)

CI/R+5 mA EA (group C)

CI/R+3 mA EA (group D) and CI/R+1 mA EA (group E) groups with eight rats in each group. Transient general brain ischemia was induced by four-vessel occlusion and reperfusion. The rats in group C

group D and group E were punctured and stimulated at Baihui (GV20)

Mingmen (GV4) and Zusanli (ST36) with the same intermittent and rarefaction-dense wave (30 to 50 Hz) and different electric current intensities: 5 mA

3 mA and 1 mA for 20 min after CI/R. Then the activities of Na+-K+-ATPase

SDH and LDH in mitochondria of brain tissue were measured by spectrophotometry. The ischemic cerebral cortex tissue was taken for observing the ultrastructure changes of impaired nerve cells. Compared with group A

the activities of LDH

SDH and Na+-K+-ATPase were lowerer in the group B (P<0.05 or P<0.01). However

the activities of LDH

SDH and Na+-K+-ATPase were higher in the group D than those in the group B (P<0.05 orP<0.01). In group A

the anatomical structure of the cerebral cortex cells was basically normal; in group B

the neuronal cellular structures were severely damaged

the neuronal mitochondria got swelling

the mitochondrial cristae were broken

the medullated nerve fifibers were not integrated. In group C

group D and group E

the ultrastructure of impaired neuron were improved. Group D was the best among three groups above. EA of 3 mA intensity could strengthen aerobic metabolism by elevating the activities of SDH and LDH

meanwhile maintaining the ionic equilibrium in the exterior and interior brain cell and relieving the cellular edema by reinforcing the activities of Na+-K+-ATPase.

Abstract

To observe the effects of electroacupuncture (EA) of different intensities on lactate dehydrogernase (LDH)

succinate dehydrogenase (SDH) and ATPase in brain tissue of rats with cerebral ischemia-reperfusion injury (CI/R). Forty male SD rats were uniformly randomized into sham operation group (group A)

CI/R group (group B)

CI/R+5 mA EA (group C)

CI/R+3 mA EA (group D) and CI/R+1 mA EA (group E) groups with eight rats in each group. Transient general brain ischemia was induced by four-vessel occlusion and reperfusion. The rats in group C

group D and group E were punctured and stimulated at Baihui (GV20)

Mingmen (GV4) and Zusanli (ST36) with the same intermittent and rarefaction-dense wave (30 to 50 Hz) and different electric current intensities: 5 mA

3 mA and 1 mA for 20 min after CI/R. Then the activities of Na+-K+-ATPase

the activities of LDH

SDH and Na+-K+-ATPase were lowerer in the group B (P<0.05 or P<0.01). However

the activities of LDH

SDH and Na+-K+-ATPase were higher in the group D than those in the group B (P<0.05 orP<0.01). In group A

the anatomical structure of the cerebral cortex cells was basically normal; in group B

the neuronal cellular structures were severely damaged

the neuronal mitochondria got swelling

the mitochondrial cristae were broken

the medullated nerve fifibers were not integrated. In group C

group D and group E

the ultrastructure of impaired neuron were improved. Group D was the best among three groups above. EA of 3 mA intensity could strengthen aerobic metabolism by elevating the activities of SDH and LDH

meanwhile maintaining the ionic equilibrium in the exterior and interior brain cell and relieving the cellular edema by reinforcing the activities of Na+-K+-ATPase.

关键词

cerebral ischemia/reperfusionelectroacupuncture intensityenergy metabolism

Keywords

cerebral ischemia/reperfusionelectroacupuncture intensityenergy metabolism

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