Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats

Shuai Wang; Tao Zhou; Jun-peng Zhai; Li-hua Wang; Jing Chen

doi:10.1007/s11655-013-1538-z

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Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats

Updated：2021-08-27

- Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats
- Chinese Journal of Integrative Medicine Vol. 20, Issue 11, Pages: 865-869(2014)
- Affiliations：
  
  1. Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University,Beijing,China
  2. Beijing Institute of Traditional Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by Beijing Excellent Talents Cultivation Project (No. 2010D003034000044);These authors contributed equally to this work
- DOI：10.1007/s11655-013-1538-z
  CLC：
- Published：2014，
  
  Published Online：14 October 2013，
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Wang, S., Zhou, T., Zhai, Jp. et al. Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats., Chin. J. Integr. Med. 20, 865–869 (2014). https://doi.org/10.1007/s11655-013-1538-z

Shuai Wang, Tao Zhou, Jun-peng Zhai, et al. Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats. [J]. Chinese Journal of Integrative Medicine 20(11):865-869(2014)
Wang, S., Zhou, T., Zhai, Jp. et al. Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats., Chin. J. Integr. Med. 20, 865–869 (2014). https://doi.org/10.1007/s11655-013-1538-z DOI：

Shuai Wang, Tao Zhou, Jun-peng Zhai, et al. Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats. [J]. Chinese Journal of Integrative Medicine 20(11):865-869(2014) DOI： 10.1007/s11655-013-1538-z.

摘要

To investigate the effects of Modified Sanhuang Decoction (加味三黄汤

MSD) enema on the serum tumor necrosis factor alpha (TNF-α) and colonic mucosa interleukin-1β (IL-1β)

interleukin-6 (IL-6) levels in experimental ulcerative colitis (UC) rats. Forty-five male Wistar rats were randomly divided into 4 groups: normal group (n=12)

model group (n=11)

salazosulfapyridine (SASP) group (n=11) and MSD group (n=11). The UC model was induced by 2

6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Rats in the normal group and model group were clystered with 0.9% normal saline

while in the SASP group and MSD group were clystered with SASP and MSD enema

respectively. After drug administration (10 mL/kg body weight

for 7 days)

colonic gross changes and colonic mucosa histology were observed

serum TNF-α and colonic mucosa IL-1β

IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay

respectively. As compared with the normal group

the experimental UC rats

the colonic mucosal damage index scores (CMDIs)

histopathological scores (HS) and the serum TNF-α and colonic mucosa IL-1β

IL-6 levels significantly increased (P<0.05 or P<0.01). In the MSD and SASP groups

the ulcer area significantly reduced

and edema disappeared. The CMDIs

the serum TNF-α and colonic mucosa IL-1β

IL-6 levels in the MSD and SASP groups significantly decreased (P<0.05 or P<0.01) compared with the model group. The CMDIs in the MSD group were lower than that in the SASP group (P<0.05)

but there were no significant differences in HS

serum TNF-α or colonic mucosa IL-1β

IL-6 levels between the MSD and SASP groups. MSD enema can improve colonic mucosa impairment and decrease serum TNF-α and colonic mucosa IL-1β

IL-6 levels in experimental UC.

Abstract

To investigate the effects of Modified Sanhuang Decoction (加味三黄汤

MSD) enema on the serum tumor necrosis factor alpha (TNF-α) and colonic mucosa interleukin-1β (IL-1β)

interleukin-6 (IL-6) levels in experimental ulcerative colitis (UC) rats. Forty-five male Wistar rats were randomly divided into 4 groups: normal group (n=12)

model group (n=11)

salazosulfapyridine (SASP) group (n=11) and MSD group (n=11). The UC model was induced by 2

6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Rats in the normal group and model group were clystered with 0.9% normal saline

while in the SASP group and MSD group were clystered with SASP and MSD enema

respectively. After drug administration (10 mL/kg body weight

for 7 days)

colonic gross changes and colonic mucosa histology were observed

serum TNF-α and colonic mucosa IL-1β

IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay

respectively. As compared with the normal group

the experimental UC rats

the colonic mucosal damage index scores (CMDIs)

histopathological scores (HS) and the serum TNF-α and colonic mucosa IL-1β

IL-6 levels significantly increased (P<0.05 or P<0.01). In the MSD and SASP groups

the ulcer area significantly reduced

and edema disappeared. The CMDIs

the serum TNF-α and colonic mucosa IL-1β

IL-6 levels in the MSD and SASP groups significantly decreased (P<0.05 or P<0.01) compared with the model group. The CMDIs in the MSD group were lower than that in the SASP group (P<0.05)

but there were no significant differences in HS

serum TNF-α or colonic mucosa IL-1β

IL-6 levels between the MSD and SASP groups. MSD enema can improve colonic mucosa impairment and decrease serum TNF-α and colonic mucosa IL-1β

IL-6 levels in experimental UC.

关键词

Ulcerative ColitisChinese herbal therapytumor necrosis factor-αinterleukin-1βinterleukin-6

Keywords

Ulcerative ColitisChinese herbal therapytumor necrosis factor-αinterleukin-1βinterleukin-6

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Department of Traditional Chinese Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University

Department of Hepatology, Zhejiang Hospital of Traditional Chinese Medicine

Department of the Integrated Chinese and Western Medicine, Shaanxi Province Traditional Chinese Medical Key Department of Nephrology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University

Department of Hematology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University

Department Emergency of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology

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