Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway

Jian-hua Huang; Wai-jiao Cai; Xin-min Zhang; Zi-yin Shen

doi:10.1007/s11655-013-1583-7

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Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway

Updated：2021-08-27

- Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway
- Chinese Journal of Integrative Medicine Vol. 20, Issue 2, Pages: 107-115(2014)
- Affiliations：
  
  Institute of Integrated Traditional Chinese Medicine and Western Medicine, Huashan Hospital, Fudan University,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program in China (No. 2007CB507400) and partially by the Project of National Natural Science Foundation of China (No. 30873319 and 30901888);These authors contributed equally to this work
- DOI：10.1007/s11655-013-1583-7
  CLC：
- Published：2014，
  
  Published Online：6 March 2014，
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Huang, Jh., Cai, Wj., Zhang, Xm. et al. Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway., Chin. J. Integr. Med. 20, 107–115 (2014). https://doi.org/10.1007/s11655-013-1583-7

Jian-hua Huang, Wai-jiao Cai, Xin-min Zhang, et al. Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway. [J]. Chinese Journal of Integrative Medicine 20(2):107-115(2014)
Huang, Jh., Cai, Wj., Zhang, Xm. et al. Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway., Chin. J. Integr. Med. 20, 107–115 (2014). https://doi.org/10.1007/s11655-013-1583-7 DOI：

Jian-hua Huang, Wai-jiao Cai, Xin-min Zhang, et al. Icariin promotes self-renewal of neural stem cells: An involvement of extracellular regulated kinase signaling pathway. [J]. Chinese Journal of Integrative Medicine 20(2):107-115(2014) DOI： 10.1007/s11655-013-1583-7.

摘要

To investigate the effects and underlying molecular mechanisms of icariin (ICA) on self-renewal and differentiation of neural stem cells (NSCs). NSCs were derived from forebrains of mice embryos by mechanical dissociation into single cell suspension. The self-renewal of NSCs was measured by neurosphere formation assay. The proliferation of NSCs was detected by water-soluble tetrazolium (WST) and 5-ethynyl-2′-deoxyuridine (EdU) incorporation assay. Protein expression of neuron-specific marker tubulin-βIII(TuJ1) and astrocyte-specific marker glial fibrillary acidic protein (GFAP) were measured by immunofluorescence and Western blotting. Using microarray

the differentially expressed genes (DEGs) were screened between NSCs with or without ICA treatment. The signaling pathways enriched by these DEGs and their role in mediating effects of ICA were analyzed. ICA significantly promoted neurosphere formation of NSCs cultured in growth protocol in a dose-dependent manner and achieved the maximum effects at 100 nmol/L. ICA also increased optical absorbance value and EdU incorporation into nuclei of NSCs. ICA had no significant effects on the percentage of TuJ1 or GFAP-positive cells

and TuJ1 or GFAP protein expression in NSCs cultured in differentiation protocol. A total of 478 genes were found to be differentially regulated. Among signaling pathways significantly enriched by DEGs

mitogen activated protein kinase (MAPK) pathway was of interest. Blockade of extracellular signal-regulated kinase (ERK)/MAPK

other than p38/MAPK subfamily pathway partially abolished effects of ICA on neurosphere formation and EdU incorporation of NSCs. ICA can promote the selfrenewal of NSCs at least partially through ERK/MAPK signaling pathway.

Abstract

mitogen activated protein kinase (MAPK) pathway was of interest. Blockade of extracellular signal-regulated kinase (ERK)/MAPK

关键词

icariinneural stem cellsself-renewaldifferentiationextracellular signaling-regulated kinase

Keywords

icariinneural stem cellsself-renewaldifferentiationextracellular signaling-regulated kinase

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