FOLLOWUS
Effects of Fengbaisan (丰白散) on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with chronic obstructive pulmonary disease
- Chinese Journal of Integrative Medicine Vol. 20, Issue 3, Pages: 224-231(2014)
- Affiliations：
1. Department of Traditional Chinese Medicine, the 2nd Xiangya Hospital, Central South University,Hunan,Changsha,China
2. Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University,Hunan,Changsha,China
- Author bio：
- Funds：Supported by the Scientific Research Foundation of Traditional Chinese Medicine of Hunan Provincial Health Bureau (No. 201194);the General Science and Technology Project of Hunan Provincial Science and Technology Bureau (No. 2011SK3232)
DOI：10.1007/s11655-013-1619-z
CLC：Published：2014，
Published Online：16 November 2013，
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Wang, Y., Su, Nx., Chen, Zq. et al. Effects of Fengbaisan (丰白散) on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with chronic obstructive pulmonary disease., Chin. J. Integr. Med. 20, 224–231 (2014). https://doi.org/10.1007/s11655-013-1619-z
Yu Wang, Nan-xiang Su, Ze-qi Chen, et al. Effects of Fengbaisan (丰白散) on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with chronic obstructive pulmonary disease. [J]. Chinese Journal of Integrative Medicine 20(3):224-231(2014)
Wang, Y., Su, Nx., Chen, Zq. et al. Effects of Fengbaisan (丰白散) on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with chronic obstructive pulmonary disease., Chin. J. Integr. Med. 20, 224–231 (2014). https://doi.org/10.1007/s11655-013-1619-z DOI：
Yu Wang, Nan-xiang Su, Ze-qi Chen, et al. Effects of Fengbaisan (丰白散) on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with chronic obstructive pulmonary disease. [J]. Chinese Journal of Integrative Medicine 20(3):224-231(2014) DOI： 10.1007/s11655-013-1619-z.
摘要
To observe effects of Fengbaisan (丰白散
FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of FBS. The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The typical histopathological changes of COPD were displayed in the model group
Ambroxol Hydrochloride group and FBS group
and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P<0.05). After treatment for successive 28 days
the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P<0.05). FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats
and this may be a possible therapeutic mechanism of FBS on COPD.
Abstract
To observe effects of Fengbaisan (丰白散
FBS) on the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in lung tissue of rats with chronic obstructive pulmonary disease (COPD) and to investigate the preventive and therapeutic mechanisms of FBS. The COPD rat model was established by cigarette smoke exposure and lipopolysaccharide (LPS) intra-tracheal dripping. The histopathological changes of lung tissue was observed via hematoxylin/eosin staining. The expression of MMP-9 and TIMP-1 in lung tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The typical histopathological changes of COPD were displayed in the model group
Ambroxol Hydrochloride group and FBS group
and the pathological lesions in the FBS group were less than those in the model group. The expression of MMP-9 and TIMP-1 in the model group increased significantly compared with those in the normal group (P<0.05). After treatment for successive 28 days
the expression of MMP-9 and TIMP-1 in the FBS group decreased remarkably as compared with the model group (P<0.05). FBS can regulate MMP-9/TIMP-1 imbalance to prevent airway and lung parenchyma remodeling process via reducing the expression of MMP-9 and TIMP-1 in the lung tissue of COPD rats
and this may be a possible therapeutic mechanism of FBS on COPD.
关键词
Chronic Obstructive Pulmonary DiseaseFengbaisanmatrix metalloproteinase-9tissue inhibitor of metalloproteinase-1airway remodelingChinese Medicine
Keywords
Chronic Obstructive Pulmonary DiseaseFengbaisanmatrix metalloproteinase-9tissue inhibitor of metalloproteinase-1airway remodelingChinese Medicine
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